Team:SUSTC-Shenzhen-B/lab results

From 2012.igem.org

(Difference between revisions)
 
(6 intermediate revisions not shown)
Line 395: Line 395:
                         <ul id="topnav" class="sf-menu">
                         <ul id="topnav" class="sf-menu">
                             <li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B">Home</a></li>
                             <li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B">Home</a></li>
-
                             <li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/lab results">Software</a>
+
                             <li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/project.overview">Project Overview</a></li>
 +
                            <li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/introduction">Software</a>
                             <ul>
                             <ul>
-
                                     <li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/lab results">Overview</a></li>
+
                                     <li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/introduction">Overview</a></li>
                                     <li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/algorithm">Algorithm</a></li>
                                     <li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/algorithm">Algorithm</a></li>
-
                                     <li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/achievements">Achievements</a></li>
+
                                     <li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/achievements">Results</a></li>
                                     <li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/Download">Download</a></li>
                                     <li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/Download">Download</a></li>
                                     <li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/comment">Comment</a></li>
                                     <li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/comment">Comment</a></li>
Line 448: Line 449:
                         </ul><!-- topnav -->
                         </ul><!-- topnav -->
                     </nav><!-- nav -->
                     </nav><!-- nav -->
-
                   
+
                    
-
                    <ul id="sn">
+
-
                     <li><a href="http://twitter.com" title="Twitter"><span class="icon-img" style="background:url(/wiki/images/1/1a/Sustc-b1-social-twitter.png)"></span></a></li>
+
-
                        <li><a href="http://plus.google.com" title="Google+"><span class="icon-img" style="background:url(/wiki/images/7/70/Sustc-b1-social-google.png)"></span></a></li>
+
-
                        <li><a href="http://facebook.com" title="Facebook"><span class="icon-img" style="background:url(/wiki/images/a/a7/Sustc-b1-social-facebook.png)"></span></a></li>
+
-
                        <li><a href="http://pinterest.com" title="pinterest"><span class="icon-img" style="background:url(/wiki/images/b/bb/Sustc-b1-social-pinterest.png)"></span></a></li>
+
-
                    </ul>
+
                     <div class="clear"></div>
                     <div class="clear"></div>
                 </section>
                 </section>
Line 475: Line 470:
         <font face="Arial, Helvetica"><br>
         <font face="Arial, Helvetica"><br>
                 </font><h3 class="STYLE2"><font face="Arial, Helvetica">1.  Fluorescence microscope results</font></h3>
                 </font><h3 class="STYLE2"><font face="Arial, Helvetica">1.  Fluorescence microscope results</font></h3>
-
Our plasmid design contains a RFP and GFP. Fluorescence microscope pictures (Figure 1 and Figure 2) show that both GFP and RFP are expressed.  
+
<p>Our plasmid design contains a RFP and GFP. Fluorescence microscope pictures Figure 1 shows that GFP is expressed.<p>
-
                <p class="STYLE2"><font face="Arial, Helvetica"><img src=" https://static.igem.org/mediawiki/2012/d/d2/71.png" class="img_fl img_border" //font></p>
+
<img src=" https://static.igem.org/mediawiki/2012/e/ec/81.png" class="img_fl img_border" >
 +
              <p><span class="STYLE3">(Figure 1 Those bacteria who express GFP is seen under the fluorescence microscope activated by 488nm light.The  picture shows the expression of the GFP. ) </span></p>
-
              <p><span class="STYLE3">(Figure 1: Those bacteria who express GFP and  RFP are seen under the fluorescence microscope activated by 488nm light.The  picture shows the expression of the RFP. )</span></p>
+
<h3 class="STYLE2"><font face="Arial, Helvetica">2.Flow cytometry results </font></h3>
-
<p class="STYLE2"><font face="Arial, Helvetica"><img src=" https://static.igem.org/mediawiki/2012/e/ec/81.png" class="img_fl img_border" //font></p>
+
<img src=" https://static.igem.org/mediawiki/2012/6/60/Lab_results-f1.jpg" class="img_fl img_border" >
-
 
+
<p><span class="STYLE3">Figure2-a</span></p>
-
              <p><span class="STYLE3">(Figure 2: Those bacteria who express GFP and  RFP are seen under the fluorescence microscope activated by 488nm light.The  picture shows the expression of the GFP. ) </span></p>
+
<img src=" https://static.igem.org/mediawiki/2012/6/60/Lab_results-f2.jpg" class="img_fl img_border" >
-
              <p>&nbsp;</p>
+
<p><span class="STYLE3">Figure2-b</span></p>
-
              <h3 class="STYLE2"><font face="Arial, Helvetica">2. Fit curves relating to dscores and terminator  efficiencies. </font></h3>
+
<p>Flow cytometry results of GFP expression in experiment group Figure2-a and control group Figure2-b.The average GFP fluorescence strength of control group is 432 unit and of experiment group is 251 unit. Therefore, the terminator efficiency is 251/432=0.58</p>
-
<p class="STYLE4"><span class="STYLE2"><img src=" https://static.igem.org/mediawiki/2012/6/6d/QQ%E6%88%AA%E5%9B%BE20120927115928.png" class="img_fl img_border" //font /></span></p>
+
<h3 class="STYLE2"><font face="Arial, Helvetica">3.Agreement with theoretical prediction</font></h3>
-
<p class="STYLE3">(figure 6:  Summary of results)</p>
+
<p class="STYLE3"><span class="STYLE2"><img src="https://static.igem.org/mediawiki/2012/d/d5/Labresult.jpg" class="img_fl img_border" //font /></span></p>
-
<p class="STYLE2"><img src="https://static.igem.org/mediawiki/2012/e/ec/91.png" class="img_fl img_border" //font /></p>
+
<p class="STYLE3">(Figure 3 The horizontal ordinate represents the value of efficiencies measured by experiment. The vertical ordinate represents the value of efficiencies predicted by TTEC. We can see a good agreement between experimental efficiency and TTEC predicted efficiency.)</p>
-
<p class="STYLE3">(figure 3 : According the data on biofab ( <a href="http://io.biofab.org/services/studio/dac/">http://io.biofab.org/services/studio/dac/</a> ) and  our software predicted dscores,  create fit curves. Linear fit curve is founded to have the highest accuracy  among all the fit curves. )</p>
+
-
<p class="STYLE3"><span class="STYLE2"><img src=" https://static.igem.org/mediawiki/2012/d/d6/92.png" class="img_fl img_border" //font /></span></p>
+
-
<p class="STYLE3">(figure 4 : According to our  experiment measured terminator efficiencies and our software predicted dscores,  we create fit curve to relate all the data . To correspond to the figure 3, we  choose linear fit curve to link all the data. There are 5 valuable points of terminator  efficiencies. The sequences are listed below. In the experiment, we transform 9  terminators to plasmid mutant-psb1a3-GF  and only get 5 reliably terminators’ data. The points appeared on the figure  are terminators whose number is 1,4,6,7,8 )</p>
+
<p class="STYLE4"><span class="STYLE2"><img src=" https://static.igem.org/mediawiki/2012/2/2b/94.png" class="img_fl img_border" //font /></span></p>
<p class="STYLE4"><span class="STYLE2"><img src=" https://static.igem.org/mediawiki/2012/2/2b/94.png" class="img_fl img_border" //font /></span></p>
-
<p class="STYLE3">(figure 5 : Here listed the sequences of  terminator whose efficiencies are measured in the lab.)</p>
+
<p class="STYLE3">(Figure 4 Here listed the sequences of  terminator whose efficiencies are measured in the lab.)</p>
<br>  
<br>  

Latest revision as of 21:18, 26 October 2012

Title

Result


1. Fluorescence microscope results

Our plasmid design contains a RFP and GFP. Fluorescence microscope pictures Figure 1 shows that GFP is expressed.

(Figure 1 Those bacteria who express GFP is seen under the fluorescence microscope activated by 488nm light.The picture shows the expression of the GFP. )

2.Flow cytometry results

Figure2-a

Figure2-b

Flow cytometry results of GFP expression in experiment group Figure2-a and control group Figure2-b.The average GFP fluorescence strength of control group is 432 unit and of experiment group is 251 unit. Therefore, the terminator efficiency is 251/432=0.58

3.Agreement with theoretical prediction

(Figure 3 The horizontal ordinate represents the value of efficiencies measured by experiment. The vertical ordinate represents the value of efficiencies predicted by TTEC. We can see a good agreement between experimental efficiency and TTEC predicted efficiency.)

(Figure 4 Here listed the sequences of terminator whose efficiencies are measured in the lab.)