Team:SUSTC-Shenzhen-B/introduction

From 2012.igem.org

(Difference between revisions)
Line 11: Line 11:
         <div id="templatemo_main">
         <div id="templatemo_main">
             <div class="col col23">
             <div class="col col23">
-
                 <h2>Welcome To TTEC!</h2>
+
                 <h2>Introduction</h2>
-
                <p>It is important that transcription is imperfectly terminated at some terminators so that the ratio of the amount of mRNA transcribed from upstream and that from downstream of the terminator is controlled. This is qualified as terminator efficiency.          </p>
+
<p>Our software is for calculating transcription terminator efficiency based on three main algorithms which are updated by our own experiment data that can be divided into two parts: software and website.</p>  
-
                <p>We studied the Transcription Terminators which regulate the gene expression. We have developed a free energy based theoretical model to calculate the terminator efficiency from nucleotide sequences. Then, we did some experiment and measured the efficiency of more than 100 terminators to validate our model. Our work is by far the most comprehensive study on terminator efficiency.</p>
+
 
-
                <p> This is the main page of our wiki. If you want to know more about our project please see detailed parts. Have a nice time!</p>
+
<p>We use three main algorithms of calculating which were put forward by different papers. They are all based on calculating the score or Gibbs free energy of the stem-loop and T-tail, and then we use some of our own experiment data of those typical terminators to get the fitting function of terminator efficiency with the score and ΔG. We also use other experment data to prove and correct our function to make it better.</p>
-
                <div class="cleaner h40"></div>
+
 
-
                <h3>Team Abstract</h3>
+
<p>The function of the software is brief that users only need to input the sequency of their terminator and then it can output the structure and efficiency of the terminator. For those users who can connect to the internet, we have our website www.terminatorefficiency.com for them to use without download our software, and for those who cannot, they can download our software to use.</p>
-
              <img src="http://2012.igem.org/wiki/images/2/26/Team_member.JPG" alt="" class="img_fl img_border" />
+
                  
-
                <p>Our team consists of 7members, who are all sophomores of South University of Science and Technology of China. We are receiving the general education and don’t have our own major now, We gather because of the same hope, the unity.</p>
+
-
                <a href="http://2012.igem.org/Team:SUSTC-Shenzhen-B/members">More</a>
+
-
                <div class="cleaner h40"></div>
+
-
                <h3>Human Practice</h3>
+
-
              <img src="http://www.templatemo.com/templates/templatemo_353_beauty_class/images/templatemo_image_02.jpg" alt="" class="img_fl img_border" />
+
-
                <p>Human practice is one of the IGEM activities that promote public awareness and education of synthetic biology and genetic engineering. As one of the IGEM team, we should contribute our share to the historic events----synthetic biology.</p>
+
-
                 <a href="http://2012.igem.org/Team:SUSTC-Shenzhen-B">More</a>
+
             </div>
             </div>
             <div class="col col13 no_margin_right">
             <div class="col col13 no_margin_right">

Revision as of 06:46, 8 September 2012

SUSTC iGEM Theme - Free CSS Template

SUSTC iGEM Theme - Free CSS Template

Introduction

Our software is for calculating transcription terminator efficiency based on three main algorithms which are updated by our own experiment data that can be divided into two parts: software and website.

We use three main algorithms of calculating which were put forward by different papers. They are all based on calculating the score or Gibbs free energy of the stem-loop and T-tail, and then we use some of our own experiment data of those typical terminators to get the fitting function of terminator efficiency with the score and ΔG. We also use other experment data to prove and correct our function to make it better.

The function of the software is brief that users only need to input the sequency of their terminator and then it can output the structure and efficiency of the terminator. For those users who can connect to the internet, we have our website www.terminatorefficiency.com for them to use without download our software, and for those who cannot, they can download our software to use.

Have a Try!

This is the address of our “transcriptional terminator efficiency calculator” website. Please input the terminator sequence and then choose an algorithm. When you press the button down it can predict the terminator efficiency and structure.

acknowledgements

This is the first time that we join this competition, we got many help from some companies and other teams. thanks for their help and supports!