# Team:SUSTC-Shenzhen-B/Tutorial

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Tutorial

Tutorial

1. http://www.terminatorefficiency.com/

1. http://www.terminatorefficiency.com/

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2. Enter a sequence (or click "example")

2. Enter a sequence (or click "example")

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3.a-Click "Algorithm1"

3.a-Click "Algorithm1"

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3.b-Click "Algorithm2"

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3.b-Click "Algorithm2"

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3.c-Click "Algorithm3"

3.c-Click "Algorithm3"

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Our software is for calculating transcription terminator efficiency based on three main algorithms which are updated by our own experiment data that can be divided into two parts: software and website.

Our software is for calculating transcription terminator efficiency based on three main algorithms which are updated by our own experiment data that can be divided into two parts: software and website.

## Revision as of 15:24, 22 September 2012

SUSTC iGEM Theme - Free CSS Template

SUSTC iGEM Theme - Free CSS Template

## Tutorial

#### 3.c-Click "Algorithm3"

Our software is for calculating transcription terminator efficiency based on three main algorithms which are updated by our own experiment data that can be divided into two parts: software and website.

We use three main algorithms of calculating which were put forward by different papers. They are all based on calculating the score or Gibbs free energy of the stem-loop and T-tail, and then we use some of our own experiment data of those typical terminators to get the fitting function of terminator efficiency with the score and ΔG. We also use other experment data to prove and correct our function to make it better.

The function of the software is brief that users only need to input the sequency of their terminator and then it can output the structure and efficiency of the terminator. For those users who can connect to the internet, we have our website www.terminatorefficiency.com for them to use without download our software, and for those who cannot, they can download our software to use.

### Tools

Because we are a software producer, we need to use different tools to write our software. First, our algorithm were written using c# in vs2010, in order to get the aim that the software can run in the back of the user interface. Second, the software user interface was wrote in vs2010 using WPF directly which we thought is the easiest and most beautiful technology for windows software. The first and second version of the website was made by three software: Photoshop, which was used for getting great picture, Dreamwaver, which was used for making the structure of the interface, and vs2010, which was used for connecting the UI with our algorithm. When everything was done, we applied our own realm name and server to achieve the dream of building the website. Although the processes of debugging and building the server were very hard, we make our software successfully.

## Online Tool

This is the address of our “transcriptional terminator efficiency calculator” website.

### acknowledgements

This is the first time that we join this competition, we got many help from some companies and other teams. thanks for their help and supports!