Team:SUSTC-Shenzhen-B

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Template:SUSTC-B1 - 2012.igem.org

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Title

Romantic Getaway

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Family Getaway

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Suite Getaway

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Welcome

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Nullam vel lacus et velit placerat convallis vitae in lorem. Fusce massa risus, viverra ut dapibus ut, tempus sit amet metus. Duis in elit nisi. In hac habitasse platea dictumst.

1

Design Ideas

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2

Problem Areas

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3

Green Garden

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4

Planting Areas

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SUSTC iGEM Theme - Free CSS Template

Transcriptional Terminator Efficiency Calculator(TTEC)

We studied the rho-independent transcription terminators which regulate gene expression using both theoretical model and experiment method.

We first developed a theoretical model. This model calculated the free energy of RNA folding and could predict the secondary structure of terminators. From the secondary structure, we proposed an algorithm that can predict terminator efficiency.

To validate our theoretical prediction, we also perform wetlab experiments. We construct 100 terminators. We measure the the terminator efficiency by measuring the GFP and RFP which are placed before and after terminators.

We also developed a software and a web server for people to calculate their terminators and built a database of terminator efficiency which we believe to be the largest database of such kind. Our work is by far the most comprehensive study on terminator efficiency.