Team:SUSTC-Shenzhen-B

From 2012.igem.org

(Difference between revisions)
Line 12: Line 12:
             <div class="col col23">
             <div class="col col23">
<font face="Arial, Helvetica">   
<font face="Arial, Helvetica">   
-
                 <h2><p>Transcriptional Terminator Efficiency Calculator(TTEC)</p></h2>
+
                 <h2><p>Transcriptional Terminator Efficiency Calculator (TTEC)</p></h2>
                 <p>We studied the rho-independent transcription terminators which regulate gene expression using both theoretical model and experiment method.</p>
                 <p>We studied the rho-independent transcription terminators which regulate gene expression using both theoretical model and experiment method.</p>

Revision as of 22:12, 26 September 2012

Template:SUSTC-B1 - 2012.igem.org

Template:SUSTC-B1

From 2012.igem.org

Title

Romantic Getaway

Nunc tortor ante, accumsan vel malesuada vulputate, tempus quis dolor. In ut purus nulla. Etiam tincidunt pharetra metus eget ultricies. Integer mi ante, laoreet cursus.

Read More

Family Getaway

Nunc tortor ante, accumsan vel malesuada vulputate, tempus quis dolor. In ut purus nulla. Etiam tincidunt pharetra metus eget ultricies. Integer mi ante, laoreet cursus.

Read More

Suite Getaway

Nunc tortor ante, accumsan vel malesuada vulputate, tempus quis dolor. In ut purus nulla. Etiam tincidunt pharetra metus eget ultricies. Integer mi ante, laoreet cursus.

Read More

Welcome

Mauris malesuada ultrices consectetur. Suspendisse varius ante quis mauris gravida auctor. Nam cursus felis non ipsum feugiat ullamcorper. Nunc ac aliquet eros.

Nam eget justo velit. Vestibulum dignissim vestibulum volutpat. Morbi quam odio, facilisis ut scelerisque id, pretium blandit diam. Suspendisse quam magna, sollicitudin vitae volutpat et, porttitor ut sem. Mauris nec dolor erat. Vestibulum condimentum libero ac elit rutrum pharetra. Nunc aliquet nisi et erat vulputate pellentesque.

Nullam vel lacus et velit placerat convallis vitae in lorem. Fusce massa risus, viverra ut dapibus ut, tempus sit amet metus. Duis in elit nisi. In hac habitasse platea dictumst.

1

Design Ideas

Pellentesque enim lacus, viverra eu egestas vel, imperdiet non felis. Aliquam erat volutpat. Nullam facilisis aliquet eleifend.

2

Problem Areas

Pellentesque enim lacus, viverra eu egestas vel, imperdiet non felis. Aliquam erat volutpat. Nullam facilisis aliquet eleifend.

3

Green Garden

Pellentesque enim lacus, viverra eu egestas vel, imperdiet non felis. Aliquam erat volutpat. Nullam facilisis aliquet eleifend.

4

Planting Areas

Pellentesque enim lacus, viverra eu egestas vel, imperdiet non felis. Aliquam erat volutpat. Nullam facilisis aliquet eleifend.

SUSTC iGEM Theme - Free CSS Template

Transcriptional Terminator Efficiency Calculator (TTEC)

We studied the rho-independent transcription terminators which regulate gene expression using both theoretical model and experiment method.

We first developed a theoretical model. This model calculated the free energy of RNA folding and could predict the secondary structure of terminators. From the secondary structure, we proposed an algorithm that can predict terminator efficiency.

To validate our theoretical prediction, we also perform wetlab experiments. We construct 100 terminators. We measure the the terminator efficiency by measuring the GFP and RFP which are placed before and after terminators.

We also developed a software and a web server for people to calculate their terminators and built a database of terminator efficiency which we believe to be the largest database of such kind. Our work is by far the most comprehensive study on terminator efficiency.