Team:SJTU-BioX-Shanghai/Project/project2.3
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We recruited naphthalene degradation pathway in ''Pseudomonas'' species, which has been well characterized. Six crucial enzymes are involved in naphthalene degradation pathway. | We recruited naphthalene degradation pathway in ''Pseudomonas'' species, which has been well characterized. Six crucial enzymes are involved in naphthalene degradation pathway. | ||
- | [[File:12SJTU_PAHbiodegradation.png|thumb| | + | [[File:12SJTU_PAHbiodegradation.png|thumb|400px|center|Demonstration of naphthalene degradation pathway in ''Pseudomonas'' species]] |
In the first catabolic step, an oxygen molecule is introduced at the 1,2-position of the aromatic nucleus to produce cis-1,2-dihydroxy-1,2-dihydronaphthalene by naphthalene dihydrodiol dioxygenase. cis-1,2-Dihydroxy-1,2-dihydronaphthalene is then dehydrogenated to 1,2-dihydroxynaphthalene by cis-naphthalene dihydrodiol dehydrogenase. 1,2-Dihydroxynaphthalene is cleaved by 1,2-dihydroxynaphthalene dioxygenase, and the resulting ring-cleavage product spontaneously cyclizes to form 2-hydroxy-2H-chromene-2-carboxylic acid. Enzymatic reactions by an isomerase and a hydratase-aldolase result in the production of salicylaldehyde, which is then transformed to salicylate by salicyladehyde dehydrogenase. | In the first catabolic step, an oxygen molecule is introduced at the 1,2-position of the aromatic nucleus to produce cis-1,2-dihydroxy-1,2-dihydronaphthalene by naphthalene dihydrodiol dioxygenase. cis-1,2-Dihydroxy-1,2-dihydronaphthalene is then dehydrogenated to 1,2-dihydroxynaphthalene by cis-naphthalene dihydrodiol dehydrogenase. 1,2-Dihydroxynaphthalene is cleaved by 1,2-dihydroxynaphthalene dioxygenase, and the resulting ring-cleavage product spontaneously cyclizes to form 2-hydroxy-2H-chromene-2-carboxylic acid. Enzymatic reactions by an isomerase and a hydratase-aldolase result in the production of salicylaldehyde, which is then transformed to salicylate by salicyladehyde dehydrogenase. | ||
Revision as of 12:52, 22 October 2012
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