Team:SJTU-BioX-Shanghai/Project/project2.3
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===Biodesulfurization of Dibenzothiophene (DBT) === | ===Biodesulfurization of Dibenzothiophene (DBT) === | ||
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+ | ==Background== | ||
+ | We found that many iGEM teams wanted to express enzymes of biodegradation pathway in E.coli. However, it doesn’t have significant superiority compared to traditional strain screening. The power of synthetic biology is not fully demonstrated here. | ||
+ | We got inspired by project of 2012 iGEM team Calgary. One of their goals is to achieve desulfuration of dibenzothiophene in E.coli. To improve the system, we aimed to link those enzymes to orderly organized membrane anchors, which would enhance desulfurization pathway remarkably. | ||
+ | Sulfur in crude oil could contribute to global warming, acid rain, and various health issues. Biodesulfurization of fossil fuel could help upgrade the quality of fossil fuel and thus the whole environment. | ||
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+ | ==Desulfurization Pathway== | ||
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+ | Four enzymes are involved in Desulfurization pathway. Dibenzothiophene monooxygenase (DszC) is responsible for converting DBT to DBT-sulfoxide and finally to DBT-sulfone (DBTO2). DBT-sulfone monooxygenase (DszA) then carries out the next step in the pathway, producing 2-hydroxybiphenyl-2-sulfinic acid (HBPS). HBPS is then converted to the final product by HBPS desulfinase (DszB), producing 2-HBP. The sulfur is released from the hydrocarbon in the form of sulfite. Oxidoreductase (DszD) uses NADH to recycle the FMNH2, allowing the whole reaction to proceed. | ||
+ | [File:Ucalgary team sulfur 4s enzyme pathway diagram.png|400px|thumb|The 4S Desulfurization Pathway, showing the desulfurization of the model compound DBT by DszA, DszB, DszC, and DszD. Credits to 2012 iGEM team Calgary] | ||
+ | For more information, click [https://2012.igem.org/Team:Calgary/Project/OSCAR/Desulfurization] | ||
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+ | == Design== | ||
+ | We are trying to link DszA, DszB, DszC and DszD to orderly organized membrane anchor to accelerate Desulfurization process in E.coli. Due to decreased distance between those enzymes, the proceeding speed could increase sharply. | ||
==Reference== | ==Reference== | ||
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+ | Habe, H. and T. Omori (2003). "Genetics of polycyclic aromatic hydrocarbon metabolism in diverse aerobic bacteria." Bioscience, biotechnology, and biochemistry 67(2): 225-243. | ||
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Revision as of 14:32, 22 October 2012
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