Team:SJTU-BioX-Shanghai/Project/project2.3
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Polycyclic aromatic hydrocarbons (PAHs), which consist of two or more fused aromatic rings, are widespread in the environment and persist for a very long time. Some PAHs are toxic, mutagenic and carcinogenic and therefore are health hazards. Efforts have been made to screen bacteria strains that could degrade PAH. Moreover, many researchers focused on studying the mechanism of PAH biodegradation. But the rate of natural biodegradation is relatively slow. Team SJTU-BioX-Shanghai is trying to build a Membrane Accelerator to speed up biodegradation rate of PAH biodegradation process. Naphthalene degradation pathway in Pseudomonas species is well studied and thus recruited in our project to build Membrane Accelerator for PAH biodegradation. | Polycyclic aromatic hydrocarbons (PAHs), which consist of two or more fused aromatic rings, are widespread in the environment and persist for a very long time. Some PAHs are toxic, mutagenic and carcinogenic and therefore are health hazards. Efforts have been made to screen bacteria strains that could degrade PAH. Moreover, many researchers focused on studying the mechanism of PAH biodegradation. But the rate of natural biodegradation is relatively slow. Team SJTU-BioX-Shanghai is trying to build a Membrane Accelerator to speed up biodegradation rate of PAH biodegradation process. Naphthalene degradation pathway in Pseudomonas species is well studied and thus recruited in our project to build Membrane Accelerator for PAH biodegradation. | ||
- | == Degradation Pathway= | + | == Degradation Pathway== |
We recruited naphthalene degradation pathway in ''Pseudomonas'' species, which has been well characterized. Six crucial enzymes are involved in naphthalene degradation pathway. | We recruited naphthalene degradation pathway in ''Pseudomonas'' species, which has been well characterized. Six crucial enzymes are involved in naphthalene degradation pathway. | ||
- | [[File:12SJTU_PAHbiodegradation.png|thumb| | + | [[File:12SJTU_PAHbiodegradation.png|thumb|500px|center|Demonstration of naphthalene degradation pathway in ''Pseudomonas'' species]] |
In the first catabolic step, an oxygen molecule is introduced at the 1,2-position of the aromatic nucleus to produce cis-1,2-dihydroxy-1,2-dihydronaphthalene by naphthalene dihydrodiol dioxygenase. cis-1,2-Dihydroxy-1,2-dihydronaphthalene is then dehydrogenated to 1,2-dihydroxynaphthalene by cis-naphthalene dihydrodiol dehydrogenase. 1,2-Dihydroxynaphthalene is cleaved by 1,2-dihydroxynaphthalene dioxygenase, and the resulting ring-cleavage product spontaneously cyclizes to form 2-hydroxy-2H-chromene-2-carboxylic acid. Enzymatic reactions by an isomerase and a hydratase-aldolase result in the production of salicylaldehyde, which is then transformed to salicylate by salicyladehyde dehydrogenase. | In the first catabolic step, an oxygen molecule is introduced at the 1,2-position of the aromatic nucleus to produce cis-1,2-dihydroxy-1,2-dihydronaphthalene by naphthalene dihydrodiol dioxygenase. cis-1,2-Dihydroxy-1,2-dihydronaphthalene is then dehydrogenated to 1,2-dihydroxynaphthalene by cis-naphthalene dihydrodiol dehydrogenase. 1,2-Dihydroxynaphthalene is cleaved by 1,2-dihydroxynaphthalene dioxygenase, and the resulting ring-cleavage product spontaneously cyclizes to form 2-hydroxy-2H-chromene-2-carboxylic acid. Enzymatic reactions by an isomerase and a hydratase-aldolase result in the production of salicylaldehyde, which is then transformed to salicylate by salicyladehyde dehydrogenase. | ||
Revision as of 12:53, 22 October 2012
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