Team:SJTU-BioX-Shanghai/Parts5

From 2012.igem.org

(Difference between revisions)
(→Violacein)
 
(8 intermediate revisions not shown)
Line 27: Line 27:
__NOTOC__
__NOTOC__
<!----------------------------------------------------从这里开始写wiki--------------------------------->
<!----------------------------------------------------从这里开始写wiki--------------------------------->
 +
=Violacein=
 +
[[Image:12SJTU-VIO.png|450px|center]]
-
==Membrane Anchor System==
+
===[http://partsregistry.org/wiki/index.php?title=Part:BBa_K771201 VioA with Membrane Anchor 2(without MS2) (BBa_K771201)]===
-
===[http://partsregistry.org/wiki/index.php?title=Part:BBa_K771000 Membrane Achor 0 (BBa_K771000)]===
+
===[http://partsregistry.org/wiki/index.php?title=Part:BBa_K771202 VioB with Membrane Anchor 3 Guo Huaqing;(BBa_K771202)]===
-
===[http://partsregistry.org/wiki/index.php?title=Part:BBa_K771001 Membrane Achor 1 (BBa_K771001)]===
+
===[http://partsregistry.org/wiki/index.php?title=Part:BBa_K771203 VioE with Membrane Anchor 4 Guo Huaqing (BBa_K771203)]===
-
===[http://partsregistry.org/wiki/index.php?title=Part:BBa_K771002 Membrane Achor 2 (BBa_K771002)]===
+
===[http://partsregistry.org/wiki/index.php?title=Part:BBa_K771204 VioC with Membrane Anchor 5(without VVD) (BBa_K771204)]===
-
===[http://partsregistry.org/wiki/index.php?title=Part:BBa_K771003 Membrane Achor 3 (BBa_K771003)]===
+
===[http://partsregistry.org/wiki/index.php?title=Part:BBa_K771205 VIoC with Membrane Anchor 5 (BBa_K771205)]===
-
===[http://partsregistry.org/wiki/index.php?title=Part:BBa_K771004 Membrane Achor 4 (BBa_K771004)]===
+
===[http://partsregistry.org/wiki/index.php?title=Part:BBa_K771206 Viod with Membrane Anchor 6 (BBa_K771206)]===
-
===[http://partsregistry.org/wiki/index.php?title=Part:BBa_K771005 Membrane Achor 5 (BBa_K771005)]===
+
-
===[http://partsregistry.org/wiki/index.php?title=Part:BBa_K771006 Membrane Achor 6 (BBa_K771006)]===
+
-
===[http://partsregistry.org/wiki/index.php?title=Part:BBa_K771007 Membrane Achor 2 without MS2(BBa_K771007)]===
+
-
===[http://partsregistry.org/wiki/index.php?title=Part:BBa_K771008 Membrane Achor 5 without VVD(BBa_K771008)]===
+
-
===[http://partsregistry.org/wiki/index.php?title=Part:BBa_K771009 RNA D0 (BBa_K771009)]===
+
-
==Single Part of Membrane Anchor==
+
Violacein is a violet pigment produced by several bacteria through pathway of 5 related genes, vioA, vioB, vioC, vioD and vioE. It was initially discovered in Chromobacterium violaceum. This metabolite of tryptophan has such special applications as antibacterial, anti-trypanocidal, anti-ulcerogenic, and anticancer drugs.
-
===[http://partsregistry.org/wiki/index.php?title=Part:BBa_K771101 ssDsbA-β-Lactamase (BBa_K771101)]===
+
There are three branches in this pathway which could verify the efficiency of the Membrane Rudder.
-
===[http://partsregistry.org/wiki/index.php?title=Part:BBa_K771102 Lgt with Linker FL3(BBa_K771102)]===
+
 
-
===[http://partsregistry.org/wiki/index.php?title=Part:BBa_K771103 ssDsbA-PDZdomain (BBa_K771103)]===
+
In Violacein Membrane Rudder System, VioA, VioB, VioE and VioC constitutively aggregate. When blue light is present, VioD with Membrane Anchor would aggregate with complex of VioA,B,C and E, making violacein the dominnant final product.But if you turn the light off, VioD with Membrane Anchor would dissociate with VioA,B,C and E. Thus the biosynthetic pathway for deoxyviolacein is switched on.
-
===[http://partsregistry.org/wiki/index.php?title=Part:BBa_K771104 ssDsbA-PDZligand (BBa_K771104)]===
+
-
===[http://partsregistry.org/wiki/index.php?title=Part:BBa_K771105 GBD Domain (BBa_K771105)]===
+
-
===[http://partsregistry.org/wiki/index.php?title=Part:BBa_K771106 GBD Ligand (BBa_K771106)]===
+
-
===[http://partsregistry.org/wiki/index.php?title=Part:BBa_K771107 SH3 Domain (BBa_K771107)]===
+
-
===[http://partsregistry.org/wiki/index.php?title=Part:BBa_K771108 SH3 Ligand (BBa_K771108)]===
+
-
===[http://partsregistry.org/wiki/index.php?title=Part:BBa_K771109 VVD(C71V and N56K) (BBa_K771109)]===
+
-
===[http://partsregistry.org/wiki/index.php?title=Part:BBa_K771111 2xPP7 (BBa_K771111)]===
+
-
===[http://partsregistry.org/wiki/index.php?title=Part:BBa_K771112 MS2 (BBa_K771112)]===
+
-
===[http://partsregistry.org/wiki/index.php?title=Part:BBa_K771113 Split EGFP 1 (BBa_K771113)]===
+
-
===[http://partsregistry.org/wiki/index.php?title=Part:BBa_K771114 Split EGFP 2 (BBa_K771114)]===
+
-
==Fatty Acid==
 
-
==Violacein==
 
<!----------------------------------------------------到这里结束--------------------------------------->
<!----------------------------------------------------到这里结束--------------------------------------->
</td>
</td>
Line 63: Line 48:
-
{{Template:12SJTU_footer}}
 
{{Template:12SJTU_footer}}
{{Template:12SJTU_footer}}

Latest revision as of 04:06, 27 September 2012

Violacein

12SJTU-VIO.png


VioA with Membrane Anchor 2(without MS2) (BBa_K771201)

VioB with Membrane Anchor 3 Guo Huaqing;(BBa_K771202)

VioE with Membrane Anchor 4 Guo Huaqing (BBa_K771203)

VioC with Membrane Anchor 5(without VVD) (BBa_K771204)

VIoC with Membrane Anchor 5 (BBa_K771205)

Viod with Membrane Anchor 6 (BBa_K771206)

Violacein is a violet pigment produced by several bacteria through pathway of 5 related genes, vioA, vioB, vioC, vioD and vioE. It was initially discovered in Chromobacterium violaceum. This metabolite of tryptophan has such special applications as antibacterial, anti-trypanocidal, anti-ulcerogenic, and anticancer drugs. There are three branches in this pathway which could verify the efficiency of the Membrane Rudder.

In Violacein Membrane Rudder System, VioA, VioB, VioE and VioC constitutively aggregate. When blue light is present, VioD with Membrane Anchor would aggregate with complex of VioA,B,C and E, making violacein the dominnant final product.But if you turn the light off, VioD with Membrane Anchor would dissociate with VioA,B,C and E. Thus the biosynthetic pathway for deoxyviolacein is switched on.



Retrieved from "http://2012.igem.org/Team:SJTU-BioX-Shanghai/Parts5"