Team:SJTU-BioX-Shanghai/Parts3
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AleAlejandro (Talk | contribs) (→VVD(C71V and N56K) (BBa_K771109)) |
AleAlejandro (Talk | contribs) (→Split EGFP 1 (BBa_K771113) and Split EGFP 2 (BBa_K771114)) |
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===[http://partsregistry.org/wiki/index.php?title=Part:BBa_K771113 Split EGFP 1 (BBa_K771113)] and [http://partsregistry.org/wiki/index.php?title=Part:BBa_K771114 Split EGFP 2 (BBa_K771114)]=== | ===[http://partsregistry.org/wiki/index.php?title=Part:BBa_K771113 Split EGFP 1 (BBa_K771113)] and [http://partsregistry.org/wiki/index.php?title=Part:BBa_K771114 Split EGFP 2 (BBa_K771114)]=== | ||
- | + | In fluorescence complementation assay, proteins that are postulated to interact are fused to unfolded complementary fragments of EGFP and expressed in E.coli. Interaction of these proteins will bring the fluorescent fragments within proximity, allowing the reporter protein to reform in its native three-dimensional structure and emit its fluorescent signal. | |
+ | EGFP was split into two halves, named 1EGFP and 2EGFP respectively. If there is interaction between two proteins which were fused with 1EGFP and 2EGFP, it is expected that fluorescence should be observed. Otherwise, no fluorescence could be observed. | ||
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Revision as of 03:36, 27 September 2012
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