Revision as of 03:55, 27 September 2012

Membrane Anchor System

A novel device which could be used to switch direction of reactions through VVD and RNA signal. For more data, see http://partsregistry.org/wiki/index.php?title=Part:BBa_K771001.

A device designed to assemble enzymes on E.coli membrane to accelerate reactions and export products more efficiently.

To justify the localization ability of fusion membrane proteins mentioned above, we constructed a novel fusion protein called BlaLG. β-lactamase is fused to the N-terminus of Lgt and GFP is fused to the C-terminus of Lgt. The fusion protein in under control of araBAD promoter .
split EGFP 1 with Membrane Achor 2 without MS2 (BBa_K771407)
split EGFP 2 with Membrane Achor 3 (BBa_K771403)
split EGFP 1 with Membrane Achor 4 (BBa_K771404)
split EGFP 2 with Membrane Anchor 5(without VVD) Membrane Achor 5 without VVD(BBa_K771405)

@@ Line 54: / Line 54: @@
 [[Image:12SJTU-MA0.png|500px|center]]
-==GFP==
+==Membrane Achor with GFP ==
 <br/>[http://partsregistry.org/wiki/index.php?title=Part:BBa_K771401 ssDsbA-β-Bla-Lgt-GFP (BBa_K77401)]
 [[Image:12SJTU-project1-1.png|600px|center]]