Team:SJTU-BioX-Shanghai

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<tr><td align="left"> <h2><b>Abstract</b></h2>
 
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<p>This year, SJTU-BioX-Shanghai iGEM team is trying to build a “factory” on E.coli’s membrane, where enzyme assemblies can be manipulated so that biochemical reactions can be accelerated and further controlled. </p>
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<p>Our slogan: Cluster makes it faster; interaction alters the direction</p>
 
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<b>Motivation</b><br>
 
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<ol><li>There lacks compartment in prokaryotic cells, and thus engineered enzymes diffuse in the cytoplasm, which makes certain reactions proceed at a very low speed.
 
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</li><li>Divergent biochemical pathways commonly exist in all types of organisms, but it is extremely hard to artificially control and switch the directions of these reactions.
 
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</li></ol>
 
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<b>What to do</b><br>
 
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<p>We aim to attach enzymes involved in certain reactions to membrane proteins in order to fulfill goals stated below:</p>
 
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<ol><li>To accelerate reactions in certain biochemical pathway
 
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</li><li>To switch the biochemical pathway from one to the other through extracellular signal control
 
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<div class="SliderName_2Description">Featured model: <strong>Little Guo</strong></div>
 
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<p>We aimed at constructing a set of protein scaffolds on E.coli cell membrane as carriers of various enzymes. Distinct from previous DNA or RNA scaffold construction, our device limits the reaction space to a two-dimensional surface. In such system,the membrane replaced DNA or RNA scaffold as an extensive surface/scaffold for proteins to anchor without limitation of scaffold amount. </p>
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<p>By gathering downstream enzymes through interacting protein domains, the reaction can be accelerated sharply. Production of Fatty Acid was enhanced by more than 20 fold through recruiting our membrane accelerator system, which has a promising application prospect in biofuel production.</p>
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<p>More strikingly, we can switch direction of enzymatic reactions through external or internal signals with our device.</p>
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<h2><b>Membrane Engine (Accelerator)</b></h2>
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<h2><b>Membrane Switch</b></h2>
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 +
this.onmouseout = $('nav-bar1').onmouseout = function(e){
 +
var e = window.event || e;
 +
var s1 = e.toElement || e.relatedTarget;
 +
if(s1 != $('nav-bar1')){if (s1.parentNode != $('nav-bar1')){
 +
IsStop1 = false;
 +
}}
 +
}
 +
}
 +
$('nav2').onmouseover = function(){
 +
with($('nav-bar2').style){
 +
width = '0';
 +
top = this.offsetTop + this.offsetHeight + 'px';
 +
right = document.documentElement.clientWidth - this.offsetLeft - this.offsetWidth + 'px';
 +
display = 'block';
 +
}
 +
IsStop2 = true;
 +
clearTimeout(s2);
 +
Show2('nav-bar2');
 +
this.onmouseout = $('nav-bar2').onmouseout = function(e){
 +
var e = window.event || e;
 +
var s2 = e.toElement || e.relatedTarget;
 +
if(s2 != $('nav-bar2')){ if (s2.parentNode != $('nav-bar2')){
 +
IsStop2 = false;
 +
}}
 +
}
 +
}
 +
$('nav3').onmouseover = function(){
 +
with($('nav-bar3').style){
 +
width = '0';
 +
top = this.offsetTop + this.offsetHeight + 'px';
 +
right = document.documentElement.clientWidth - this.offsetLeft - this.offsetWidth + 'px';
 +
display = 'block';
 +
}
 +
IsStop3 = true;
 +
clearTimeout(s3);
 +
Show3('nav-bar3');
 +
this.onmouseout = $('nav-bar3').onmouseout = function(e){
 +
var e = window.event || e;
 +
var s3 = e.toElement || e.relatedTarget;
 +
if(s3 != $('nav-bar3')){ if (s3.parentNode != $('nav-bar3')){
 +
IsStop3 = false;
 +
}}
 +
}
 +
}
 +
$('nav4').onmouseover = function(){
 +
with($('nav-bar4').style){
 +
width = '0';
 +
top = this.offsetTop + this.offsetHeight + 'px';
 +
right = document.documentElement.clientWidth - this.offsetLeft - this.offsetWidth + 'px';
 +
display = 'block';
 +
}
 +
IsStop4 = true;
 +
clearTimeout(s4);
 +
Show4('nav-bar4');
 +
this.onmouseout = $('nav-bar4').onmouseout = function(e){
 +
var e = window.event || e;
 +
var s4 = e.toElement || e.relatedTarget;
 +
if(s4 != $('nav-bar4')){ if (s4.parentNode != $('nav-bar4')){
 +
IsStop4 = false;
 +
}}
 +
}
 +
}
 +
$('nav5').onmouseover = function(){
 +
with($('nav-bar5').style){
 +
width = '0';
 +
top = this.offsetTop + this.offsetHeight + 'px';
 +
right = document.documentElement.clientWidth - this.offsetLeft - this.offsetWidth + 'px';
 +
display = 'block';
 +
}
 +
IsStop5 = true;
 +
clearTimeout(s5);
 +
Show5('nav-bar5');
 +
this.onmouseout = $('nav-bar5').onmouseout = function(e){
 +
var e = window.event || e;
 +
var s5 = e.toElement || e.relatedTarget;
 +
if(s5 != $('nav-bar5')){ if (s5.parentNode != $('nav-bar5')){
 +
IsStop5 = false;
 +
}}
 +
}
 +
}
 +
$('nav6').onmouseover = function(){
 +
with($('nav-bar6').style){
 +
width = '0';
 +
top = this.offsetTop + this.offsetHeight + 'px';
 +
right = document.documentElement.clientWidth - this.offsetLeft - this.offsetWidth + 'px';
 +
display = 'block';
 +
}
 +
IsStop6 = true;
 +
clearTimeout(s6);
 +
Show6('nav-bar6');
 +
this.onmouseout = $('nav-bar6').onmouseout = function(e){
 +
var e = window.event || e;
 +
var s6 = e.toElement || e.relatedTarget;
 +
if(s6 != $('nav-bar6')){ if (s6.parentNode != $('nav-bar6')){
 +
IsStop6 = false;
 +
}}
 +
}
 +
}
 +
</script>
 +
</tbody></table>
 +
</div>
</td>
</td>
</tr>
</tr>
-
</tbody></table>
 
-
<!--!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!-->
 
-
<table border="0" cellspacing="0" cellpadding="0" align="left"><tbody>
 
-
<tr><td width="600px"><h2><b>Sponsors</b></h2>
 
-
<table class="sponsorstable" cellspacing="20" ;="" align="center">
 
-
<tbody><tr>
 
-
</tr>
+
</tbody></table>
 +
-
</tbody></table>
+
-
</td>
+
</div>
-
<td>
+
-
</td>
+
-
</tr>
+
-
<tbody></table>
+
</body>
</body>
</html>
</html>
-
{{Template:12SJTU_footer}}
 

Revision as of 03:48, 26 September 2012

Retrieved from "http://2012.igem.org/Team:SJTU-BioX-Shanghai"