Team:Queens Canada/Notebook/Week3

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<p>Notebook - Week 3</p>
<p>Notebook - Week 3</p>
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Week
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<li> <a href="http://2012.igem.org/Team:Queens_Canada/Notebook/Week1">1</a> </li>
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<li> <a href="http://2012.igem.org/Team:Queens_Canada/Notebook/Week2">2</a> </li>
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<li> <a href="http://2012.igem.org/Team:Queens_Canada/Notebook/Week3">3</a> </li>
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<li> <a href="http://2012.igem.org/Team:Queens_Canada/Notebook/Week4">4</a> </li>
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<li> <a href="http://2012.igem.org/Team:Queens_Canada/Notebook/Week5">5</a> </li>
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<li> <a href="http://2012.igem.org/Team:Queens_Canada/Notebook/Week6">6</a> </li>
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<li> <a href="http://2012.igem.org/Team:Queens_Canada/Notebook/Week7">7</a> </li>
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<li> <a href="http://2012.igem.org/Team:Queens_Canada/Notebook/Week8">8</a> </li>
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<li> <a href="http://2012.igem.org/Team:Queens_Canada/Notebook/Week9">9</a> </li>
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<li> <a href="http://2012.igem.org/Team:Queens_Canada/Notebook/Week10">10</a> </li>
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<li> <a href="http://2012.igem.org/Team:Queens_Canada/Notebook/Week11">11</a> </li>
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<li> <a href="http://2012.igem.org/Team:Queens_Canada/Notebook/Week12">12</a> </li>
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<li> <a href="http://2012.igem.org/Team:Queens_Canada/Notebook/Week13">13</a> </li>
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<li> <a href="http://2012.igem.org/Team:Queens_Canada/Notebook/Week14">14</a> </li>
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<li> <a href="http://2012.igem.org/Team:Queens_Canada/Notebook/Week15">15</a> </li>
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<li> <a href="http://2012.igem.org/Team:Queens_Canada/Notebook/Week16">16</a> </li>
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<li> <a href="http://2012.igem.org/Team:Queens_Canada/Notebook/Week17">17+</a> </li>
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         <div id="protocolcontent">Protocol Content</div>
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         <h1> Monday May 21</h1> <p> <h2> Today was Victoria day, a statutory holiday in Canada, so we took the day to rest and relax and prepare for the week ahead!</p> </h2> <h1>Tuesday May 22</h1> <h2> <p> First day in lab hurray! </p>
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         <h1> Monday May 14</h1>   <h2> <p> Today we held our Battle Royale for the position of Lab Manager. Weapons included an axe and PCR. After each candidate made their speech, Andrew asked them a question to measure their competency, which was some elaboration of "If I spilled HCl everywhere, what would you do?" (side note from Kevin: "This does not count towards your consideration for the position.") </p> </h2> <h1>Tuesday May 15 </h1> <h2> <p> Today we continued researching potential enzymatic pathways for our flagelar scaffold. Kevin taught us how to design primers, using tools such as the NEB cutter to check for restriction enzyme cut sites and OligoCalc to find out oligonucleotide properties. </p> </h2>  <h1> Wednesday May 16 </h1> <h2> <p> In the afternoon, we had our first faculty advisor meeting with Drs. Chin-Sang and Bendena. We concluded that characterization would take up the bulk of the time for our project work, and that we should use GFP as a proof of concept. </h2> </p> <h1> Thursday May 17 </h1> <h2> <p> Our brain food for the day was Clif bars courtesy of Mitangi. Kevin outlined four issues we had to sort out for our chimeric flagellin project, such as removing stop codons in the middle of our fusion sequence. We also started a list of BioBricks to order. In the evening, Team Flagellin' won a round of trivia at the Grad Club! Goal #1 achieved; now we can focus on goal #2: our iGEM project. </h2> </p> <h1> Friday May 18 </h1> <h2> Today we continued putting together our list of BioBricks to order. We also looked into where we could get genes synthesized. </p> </h2></div>
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<p>In the morning we went over lab training/ safety orientation, as well as completing WHMIS training, that must have been completed before we enter the lab. In the afternoon we cleaned up our assigned lab bench in preparation for our work in the lab tomorrow.</p> <h1> Wednesday May 23 </h1> <h2> <p> This morning we worked mainly on primer design in preparation for PCR work. Kevin also met with one of our advisors, Dr. Allingham in order to discussing modeling the protein design. In the afternoon, we tested different protocols on transformation of Red Fluorescent Protein (RFP) DNA biobricks into LB plates with chloramphenicol anitibiotic resistance. Four trials were currently incubated at 37 degrees celcius overnight. </h2> </p> <h1> Thursday May 24 </h1> <h2> <p> We spend the majority of the day working on sponsorship, following up on emails sent. We also spent some time in the lab. We got red colonies in the liquid medium shown as a cloudy red liquid, we can move on to miniprep (isolating the plasmid from the colonies). We spent our time in the lab today preparing for the miniprep procedure tomorrow. </h2> </p> <h1> Friday May 25 </h1> <h2> Today we worked more on sponsorship in the morning, and got into the lab in the afternoon. Performed the miniprep procedure on the liquid colonies. Involves adding a lot of different solutions as well as centrifuging. We isolated BBa_J04450 , and stored it in the fridge at -20 degrees celcius.  We also looked at the red colonies under the microscope and they did fluores. After work, Kevin and the rest of the team involved in SynthetiQ brainstormed some ideas for some videos. </p> </h2></div>
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Latest revision as of 03:20, 4 October 2012

Control

Notebook - Week 3

Protocol Content

Monday May 14

Today we held our Battle Royale for the position of Lab Manager. Weapons included an axe and PCR. After each candidate made their speech, Andrew asked them a question to measure their competency, which was some elaboration of "If I spilled HCl everywhere, what would you do?" (side note from Kevin: "This does not count towards your consideration for the position.")

Tuesday May 15

Today we continued researching potential enzymatic pathways for our flagelar scaffold. Kevin taught us how to design primers, using tools such as the NEB cutter to check for restriction enzyme cut sites and OligoCalc to find out oligonucleotide properties.

Wednesday May 16

In the afternoon, we had our first faculty advisor meeting with Drs. Chin-Sang and Bendena. We concluded that characterization would take up the bulk of the time for our project work, and that we should use GFP as a proof of concept.

Thursday May 17

Our brain food for the day was Clif bars courtesy of Mitangi. Kevin outlined four issues we had to sort out for our chimeric flagellin project, such as removing stop codons in the middle of our fusion sequence. We also started a list of BioBricks to order. In the evening, Team Flagellin' won a round of trivia at the Grad Club! Goal #1 achieved; now we can focus on goal #2: our iGEM project.

Friday May 18

Today we continued putting together our list of BioBricks to order. We also looked into where we could get genes synthesized.