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Team:Queens Canada/ChimeriQ/Results
From 2012.igem.org
Control
ChimeriQ - Results
Overview
As the goal of our project was to create chimeric flagella, the majority of our results were based around the creation of flagella with various inserts. In order to do so, we needed to make a mechanism by which we could easily create this chimeric primers. In doing so, we were able to create the part K781000 . This is the complete flagellin coding sequence with biobrick standard cut sites removed, under the promoter R0010 and RBS B0034. This part can be used to over express the flagellin monomer. PCR overlap extension will make an insertion at the annotated site, given that the insert is in the appropriate format. The resulting chimera will express protein immediately after the PCR reaction is transformed.
Fluorescence
STUFF
Catalysis
STUFF
The Cohesin-Dockering System
STUFF
