http://2012.igem.org/wiki/index.php?title=Team:Purdue/Safety&feed=atom&action=historyTeam:Purdue/Safety - Revision history2024-03-28T09:02:43ZRevision history for this page on the wikiMediaWiki 1.16.0http://2012.igem.org/wiki/index.php?title=Team:Purdue/Safety&diff=286781&oldid=prevGshowalt at 17:09, 26 October 20122012-10-26T17:09:16Z<p></p>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><b> 1. Would any of your project ideas raise safety issues in terms of </div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><b> 1. Would any of your project ideas raise safety issues in terms of researcher safety, public safety, or, environmental safety<ins class="diffchange diffchange-inline">?</ins></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline"><li> </del>researcher safety,</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline"><li> </del>public safety, or,</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline"><li> </del>environmental safety <del class="diffchange diffchange-inline"></li></del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div></div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><n></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><n></div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><b>2. Do any of the new BioBrick parts (or devices) that you made this year raise any safety issues? </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><b>2. Do any of the new BioBrick parts (or devices) that you made this year raise any safety issues? </div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><<del class="diffchange diffchange-inline">li</del>> Did you document this issues in the Registry?</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><<ins class="diffchange diffchange-inline">ul</ins>> Did you document this issues in the Registry? <ins class="diffchange diffchange-inline"></ul></ins></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><<del class="diffchange diffchange-inline">li</del>> How did you manage to handle the safety issue?</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><<ins class="diffchange diffchange-inline">ul</ins>> How did you manage to handle the safety issue? <ins class="diffchange diffchange-inline"></ul></ins></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><<del class="diffchange diffchange-inline">li</del>>How could other teams learn from your experience?</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><<ins class="diffchange diffchange-inline">ul</ins>> How could other teams learn from your experience? </<ins class="diffchange diffchange-inline">ul</ins>></div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><n> Because the constructed parts do not contain or produce silica or silica derivatives, are hosted in non-pathogenic strains, and contain no expected selective advantage, they individually pose no potential threat. The parts up-regulate natural expression of the adhesion protein Curli and attach a membrane-spanning protein to the silica-polymerizing enzyme silicatein–alpha. Out of context of biofilm attachment for water purification, the parts pose no threat to the researcher, the public or the environment. Accordingly, no safety concerns were indicated in the parts registry, although future teams may wish to further characterize the silica matrix formation in terms of detachment in water flow in respect to health effects.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><n> Because the constructed parts do not contain or produce silica or silica derivatives, are hosted in non-pathogenic strains, and contain no expected selective advantage, they individually pose no potential threat. The parts up-regulate natural expression of the adhesion protein Curli and attach a membrane-spanning protein to the silica-polymerizing enzyme silicatein–alpha. Out of context of biofilm attachment for water purification, the parts pose no threat to the researcher, the public or the environment. Accordingly, no safety concerns were indicated in the parts registry, although future teams may wish to further characterize the silica matrix formation in terms of detachment in water flow in respect to health effects.</div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><b> 3. Is there a local biosafety group, committee, or review board at your institution?</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><b> 3. Is there a local biosafety group, committee, or review board at your institution?</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><<del class="diffchange diffchange-inline">li</del>> If yes, what does your local biosafety group think about your project? </div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><<ins class="diffchange diffchange-inline">ul</ins>> If yes, what does your local biosafety group think about your project? <ins class="diffchange diffchange-inline"></ul></ins></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><<del class="diffchange diffchange-inline">li</del>> If no, which specific biosafety rules or guidelines do you have to consider in your country? </<del class="diffchange diffchange-inline">li</del>> </b></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><<ins class="diffchange diffchange-inline">ul</ins>> If no, which specific biosafety rules or guidelines do you have to consider in your country? </<ins class="diffchange diffchange-inline">ul</ins>> </b></div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Purdue University Institutional Biosafety Committee oversees the laboratories working with recombinant DNA technologies under the guidelines of the National Institutes of Health Guidelines for Research Involving Recombinant DNA Molecules, the Centers for Disease Control, and Purdue’s BioSafety Manual. Purdue’s iGEM Team functions under a Biosafety Level I designation, with all biological agents meeting the definition of Risk Group 1 and ‘unknown or minimal potential hazard to laboratory personnel and the environment’. The Purdue IBC regulates the Team and its project as such.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Purdue University Institutional Biosafety Committee oversees the laboratories working with recombinant DNA technologies under the guidelines of the National Institutes of Health Guidelines for Research Involving Recombinant DNA Molecules, the Centers for Disease Control, and Purdue’s BioSafety Manual. Purdue’s iGEM Team functions under a Biosafety Level I designation, with all biological agents meeting the definition of Risk Group 1 and ‘unknown or minimal potential hazard to laboratory personnel and the environment’. The Purdue IBC regulates the Team and its project as such.</div></td></tr>
</table>Gshowalthttp://2012.igem.org/wiki/index.php?title=Team:Purdue/Safety&diff=99278&oldid=prevGshowalt at 02:58, 7 September 20122012-09-07T02:58:10Z<p></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><b> References and More Information </b></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><b> References and More Information </b></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><li> "Aluminum and silica in drinking water and the risk of Alzheimer's disease or <del class="diffchange diffchange-inline">cognitive </del>decline: findings from 15-year follow-up of the PAQUID <del class="diffchange diffchange-inline">cohort"</del>, Virginie Rondeau, Hélène Jacqmin-Gadda, Daniel Commenges, Catherine Helmer, and Jean-François Dartigues <del class="diffchange diffchange-inline">, </del>http://www.<del class="diffchange diffchange-inline">ncbi</del>.<del class="diffchange diffchange-inline">nlm.nih.gov</del>/<del class="diffchange diffchange-inline">pmc</del>/<del class="diffchange diffchange-inline">articles</del>/<del class="diffchange diffchange-inline">PMC2809081</del>/</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><li> <ins class="diffchange diffchange-inline"><a href = </ins>"<ins class="diffchange diffchange-inline">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2809081/"> </ins>Aluminum and silica in drinking water and the risk of Alzheimer's disease or <ins class="diffchange diffchange-inline">coort"gnitive </ins>decline: findings from 15-year follow-up of the PAQUID <ins class="diffchange diffchange-inline">> </a></ins>, Virginie Rondeau, Hélène Jacqmin-Gadda, Daniel Commenges, Catherine Helmer, and Jean-François Dartigues </div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline"><li> </del>"Subchronic inhalation toxicity of amorphous silicas and quartz dust in rats<del class="diffchange diffchange-inline">"</del>, P.G.J. Reuzel, J.P. Bruijntjes, V.J. Feron, R.A. Woutersen, <del class="diffchange diffchange-inline">http://www.sciencedirect.com/science/article/pii/027869159190205L </del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline"><li> <a href= "</ins>http://www.<ins class="diffchange diffchange-inline">sciencedirect</ins>.<ins class="diffchange diffchange-inline">com</ins>/<ins class="diffchange diffchange-inline">science</ins>/<ins class="diffchange diffchange-inline">article</ins>/<ins class="diffchange diffchange-inline">pii</ins>/<ins class="diffchange diffchange-inline">027869159190205L</ins>" <ins class="diffchange diffchange-inline">> </ins>Subchronic inhalation toxicity of amorphous silicas and quartz dust in rats <ins class="diffchange diffchange-inline"></a></ins>, P.G.J. Reuzel, J.P. Bruijntjes, V.J. Feron, R.A. Woutersen, </div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><li><del class="diffchange diffchange-inline">National Institutes of Health Guildelines for Research Involving Recombinant DNA Molecules (</del>http://oba.od.nih.gov/oba/rac/guidelines/nih_guidelines.htm<del class="diffchange diffchange-inline">)</del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><li> <ins class="diffchange diffchange-inline"><a href = "</ins>http://oba.od.nih.gov/oba/rac/guidelines/nih_guidelines.htm<ins class="diffchange diffchange-inline">" > National Institutes of Health Guildelines for Research Involving Recombinant DNA Molecules </a></ins></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><li> <del class="diffchange diffchange-inline">Purdue University Institutional Biosafety Commission (</del>http://www.purdue.edu/research/vpr/rschadmin/rschoversight/rdna/index.php<del class="diffchange diffchange-inline">)</del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><li> <ins class="diffchange diffchange-inline"><a href= "</ins>http://www.purdue.edu/research/vpr/rschadmin/rschoversight/rdna/index.php<ins class="diffchange diffchange-inline">" > Purdue University Institutional Biosafety Committee</a></ins></div></td></tr>
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</table>Gshowalthttp://2012.igem.org/wiki/index.php?title=Team:Purdue/Safety&diff=99268&oldid=prevGshowalt at 02:50, 7 September 20122012-09-07T02:50:09Z<p></p>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><b> References </b></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><b> References <ins class="diffchange diffchange-inline">and More Information </ins></b></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><li> "Aluminum and silica in drinking water and the risk of Alzheimer's disease or cognitive decline: findings from 15-year follow-up of the PAQUID cohort", Virginie Rondeau, Hélène Jacqmin-Gadda, Daniel Commenges, Catherine Helmer, and Jean-François Dartigues , http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2809081/</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><li> "Aluminum and silica in drinking water and the risk of Alzheimer's disease or cognitive decline: findings from 15-year follow-up of the PAQUID cohort", Virginie Rondeau, Hélène Jacqmin-Gadda, Daniel Commenges, Catherine Helmer, and Jean-François Dartigues , http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2809081/</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><li> "Subchronic inhalation toxicity of amorphous silicas and quartz dust in rats", P.G.J. Reuzel, J.P. Bruijntjes, V.J. Feron, R.A. Woutersen, http://www.sciencedirect.com/science/article/pii/027869159190205L </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><li> "Subchronic inhalation toxicity of amorphous silicas and quartz dust in rats", P.G.J. Reuzel, J.P. Bruijntjes, V.J. Feron, R.A. Woutersen, http://www.sciencedirect.com/science/article/pii/027869159190205L </div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"><li>National Institutes of Health Guildelines for Research Involving Recombinant DNA Molecules (http://oba.od.nih.gov/oba/rac/guidelines/nih_guidelines.htm)</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"><li> Purdue University Institutional Biosafety Commission (http://www.purdue.edu/research/vpr/rschadmin/rschoversight/rdna/index.php)</ins></div></td></tr>
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</table>Gshowalthttp://2012.igem.org/wiki/index.php?title=Team:Purdue/Safety&diff=99265&oldid=prevGshowalt at 02:45, 7 September 20122012-09-07T02:45:11Z<p></p>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline"><p><span class="style1">Teams, please document any answers to these safety questions on your wiki safety page. Judges will be asked to evaluate your project, in part, on the basis of if and how you considered and addressed issues of biological safety.</span> If any questions arise regarding iGEM and biological safety please send an email to safety AT igem.org. </p></del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div> </div></td></tr>
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</table>Gshowalthttp://2012.igem.org/wiki/index.php?title=Team:Purdue/Safety&diff=99264&oldid=prevGshowalt at 02:44, 7 September 20122012-09-07T02:44:32Z<p></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>To ensure biological safety in future iGEM competitions, a program screening sequences of DNA constructs against toxicity databases to alert the presence of a known hazardous element should be developed. This would be an easy and automated ‘first-defense’ for constructed devices.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>To ensure biological safety in future iGEM competitions, a program screening sequences of DNA constructs against toxicity databases to alert the presence of a known hazardous element should be developed. This would be an easy and automated ‘first-defense’ for constructed devices.</div></td></tr>
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<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"><p></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"><b> References </b></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"><li> "Aluminum and silica in drinking water and the risk of Alzheimer's disease or cognitive decline: findings from 15-year follow-up of the PAQUID cohort", Virginie Rondeau, Hélène Jacqmin-Gadda, Daniel Commenges, Catherine Helmer, and Jean-François Dartigues , http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2809081/</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"><li> "Subchronic inhalation toxicity of amorphous silicas and quartz dust in rats", P.G.J. Reuzel, J.P. Bruijntjes, V.J. Feron, R.A. Woutersen, http://www.sciencedirect.com/science/article/pii/027869159190205L </ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"></li></ins></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p><span class="style1">Teams, please document any answers to these safety questions on your wiki safety page. Judges will be asked to evaluate your project, in part, on the basis of if and how you considered and addressed issues of biological safety.</span> If any questions arise regarding iGEM and biological safety please send an email to safety AT igem.org. </p></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p><span class="style1">Teams, please document any answers to these safety questions on your wiki safety page. Judges will be asked to evaluate your project, in part, on the basis of if and how you considered and addressed issues of biological safety.</span> If any questions arise regarding iGEM and biological safety please send an email to safety AT igem.org. </p></div></td></tr>
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</table>Gshowalthttp://2012.igem.org/wiki/index.php?title=Team:Purdue/Safety&diff=99263&oldid=prevGshowalt at 02:40, 7 September 20122012-09-07T02:40:36Z<p></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><b>2. Do any of the new BioBrick parts (or devices) that you made this year raise any safety issues? </b></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><b>2. Do any of the new BioBrick parts (or devices) that you made this year raise any safety issues? </div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline"><li> Did you document this issues in the Registry?</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline"><li> How did you manage to handle the safety issue?</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline"><li>How could other teams learn from your experience?</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline"></li></ins></b></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><n> Because the constructed parts do not contain or produce silica or silica derivatives, are hosted in non-pathogenic strains, and contain no expected selective advantage, they individually pose no potential threat. The parts up-regulate natural expression of the adhesion protein Curli and attach a membrane-spanning protein to the silica-polymerizing enzyme silicatein–alpha. Out of context of biofilm attachment for water purification, the parts pose no threat to the researcher, the public or the environment. Accordingly, no safety concerns were indicated in the parts registry, although future teams may wish to further characterize the silica matrix formation in terms of detachment in water flow in respect to health effects.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><n> Because the constructed parts do not contain or produce silica or silica derivatives, are hosted in non-pathogenic strains, and contain no expected selective advantage, they individually pose no potential threat. The parts up-regulate natural expression of the adhesion protein Curli and attach a membrane-spanning protein to the silica-polymerizing enzyme silicatein–alpha. Out of context of biofilm attachment for water purification, the parts pose no threat to the researcher, the public or the environment. Accordingly, no safety concerns were indicated in the parts registry, although future teams may wish to further characterize the silica matrix formation in terms of detachment in water flow in respect to health effects.</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><b> 3. Is there a local biosafety group, committee, or review board at your institution? </b></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><b> 3. Is there a local biosafety group, committee, or review board at your institution?</div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline"><li> If yes, what does your local biosafety group think about your project? </ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline"><li> If no, which specific biosafety rules or guidelines do you have to consider in your country? </li> </ins></b></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><n></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><n></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Purdue University Institutional Biosafety Committee oversees the laboratories working with recombinant DNA technologies under the guidelines of the National Institutes of Health Guidelines for Research Involving Recombinant DNA Molecules, the Centers for Disease Control, and Purdue’s BioSafety Manual. Purdue’s iGEM Team functions under a Biosafety Level I designation, with all biological agents meeting the definition of Risk Group 1 and ‘unknown or minimal potential hazard to laboratory personnel and the environment’. The Purdue IBC regulates the Team and its project as such.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Purdue University Institutional Biosafety Committee oversees the laboratories working with recombinant DNA technologies under the guidelines of the National Institutes of Health Guidelines for Research Involving Recombinant DNA Molecules, the Centers for Disease Control, and Purdue’s BioSafety Manual. Purdue’s iGEM Team functions under a Biosafety Level I designation, with all biological agents meeting the definition of Risk Group 1 and ‘unknown or minimal potential hazard to laboratory personnel and the environment’. The Purdue IBC regulates the Team and its project as such.</div></td></tr>
</table>Gshowalthttp://2012.igem.org/wiki/index.php?title=Team:Purdue/Safety&diff=99260&oldid=prevGshowalt at 02:37, 7 September 20122012-09-07T02:37:48Z<p></p>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>1. The bacterial strains used for research are non-pathogenic (XL-10 Gold Ultra competent Cells and DH5-alpha), indicating little risk to the research or public. The introduction of manmade biological parts into the environment is an inherent risk, mirroring those of all man-made technologies in biology: the introduction of constructed materials to the detriment of the natural environment.</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline"><b> </ins>1. <ins class="diffchange diffchange-inline">Would any of your project ideas raise safety issues in terms of </ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline"><li> researcher safety,</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline"><li> public safety, or,</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline"><li> environmental safety </li></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline"></b></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline"><n></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>The bacterial strains used for research are non-pathogenic (XL-10 Gold Ultra competent Cells and DH5-alpha), indicating little risk to the research or public. The introduction of manmade biological parts into the environment is an inherent risk, mirroring those of all man-made technologies in biology: the introduction of constructed materials to the detriment of the natural environment.</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>In addition to the bacteria, silicic acid is used to induce the formation of silica matrices on the biofilm surface. Silicic acid has no documented health effects, but its derivative, Silica, has been associated with silicosis, bronchitis, and cancer (Reuzel et. al, “Subchronic inhiliation toxicity of amorphous silicas and quartz dust in rats.” ) The context of these studies are in prolonged exposure above the Occupational Health and Safety Association limit of 0.1mg/m^3 in aersol and dust form. More relevant studies following long-term exposure to silica in water found correlation to dementia when settled with aluminum (Rondeau et. al, “Aluminum and silica in drinking water and the risk of Alzheimer’s disease…”). Because detached biofilm would polymerize added silicic acid into silica, those may pose a potential threat if consumed about OSHA recommended intake levels.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>In addition to the bacteria, silicic acid is used to induce the formation of silica matrices on the biofilm surface. Silicic acid has no documented health effects, but its derivative, Silica, has been associated with silicosis, bronchitis, and cancer (Reuzel et. al, “Subchronic inhiliation toxicity of amorphous silicas and quartz dust in rats.” ) The context of these studies are in prolonged exposure above the Occupational Health and Safety Association limit of 0.1mg/m^3 in aersol and dust form. More relevant studies following long-term exposure to silica in water found correlation to dementia when settled with aluminum (Rondeau et. al, “Aluminum and silica in drinking water and the risk of Alzheimer’s disease…”). Because detached biofilm would polymerize added silicic acid into silica, those may pose a potential threat if consumed about OSHA recommended intake levels.</div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"></n></ins></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>2. Because the constructed parts do not contain or produce silica or silica derivatives, are hosted in non-pathogenic strains, and contain no expected selective advantage, they individually pose no potential threat. The parts up-regulate natural expression of the adhesion protein Curli and attach a membrane-spanning protein to the silica-polymerizing enzyme silicatein–alpha. Out of context of biofilm attachment for water purification, the parts pose no threat to the researcher, the public or the environment. Accordingly, no safety concerns were indicated in the parts registry, although future teams may wish to further characterize the silica matrix formation in terms of detachment in water flow in respect to health effects.</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline"><b></ins>2. <ins class="diffchange diffchange-inline"> Do any of the new BioBrick parts (or devices) that you made this year raise any safety issues? </b></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline"><n> </ins>Because the constructed parts do not contain or produce silica or silica derivatives, are hosted in non-pathogenic strains, and contain no expected selective advantage, they individually pose no potential threat. The parts up-regulate natural expression of the adhesion protein Curli and attach a membrane-spanning protein to the silica-polymerizing enzyme silicatein–alpha. Out of context of biofilm attachment for water purification, the parts pose no threat to the researcher, the public or the environment. Accordingly, no safety concerns were indicated in the parts registry, although future teams may wish to further characterize the silica matrix formation in terms of detachment in water flow in respect to health effects.</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>3. Purdue University Institutional Biosafety Committee oversees the laboratories working with recombinant DNA technologies under the guidelines of the National Institutes of Health Guidelines for Research Involving Recombinant DNA Molecules, the Centers for Disease Control, and Purdue’s BioSafety Manual. Purdue’s iGEM Team functions under a Biosafety Level I designation, with all biological agents meeting the definition of Risk Group 1 and ‘unknown or minimal potential hazard to laboratory personnel and the environment’. The Purdue IBC regulates the Team and its project as such.</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline"><b> </ins>3. <ins class="diffchange diffchange-inline">Is there a local biosafety group, committee, or review board at your institution? </b></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline"><n></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>Purdue University Institutional Biosafety Committee oversees the laboratories working with recombinant DNA technologies under the guidelines of the National Institutes of Health Guidelines for Research Involving Recombinant DNA Molecules, the Centers for Disease Control, and Purdue’s BioSafety Manual. Purdue’s iGEM Team functions under a Biosafety Level I designation, with all biological agents meeting the definition of Risk Group 1 and ‘unknown or minimal potential hazard to laboratory personnel and the environment’. The Purdue IBC regulates the Team and its project as such.</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>4. To ensure biological safety in future iGEM competitions, a program screening sequences of DNA constructs against toxicity databases to alert the presence of a known hazardous element should be developed. This would be an easy and automated ‘first-defense’ for constructed devices.</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline"><b> </ins>4. <ins class="diffchange diffchange-inline"> Do you have any other ideas how to deal with safety issues that could be useful for future iGEM competitions? How could parts, devices and systems be made even safer through biosafety engineering? </b></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline"><n></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>To ensure biological safety in future iGEM competitions, a program screening sequences of DNA constructs against toxicity databases to alert the presence of a known hazardous element should be developed. This would be an easy and automated ‘first-defense’ for constructed devices.</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> </div></td></tr>
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</table>Gshowalthttp://2012.igem.org/wiki/index.php?title=Team:Purdue/Safety&diff=99252&oldid=prevGshowalt at 02:34, 7 September 20122012-09-07T02:34:21Z<p></p>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;">Use this page to answer the questions on the [[Safety | safety page]].</del></div></td><td colspan="2"> </td></tr>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><p><del class="diffchange diffchange-inline">Before answering these questions on your team Safety page</del>, <del class="diffchange diffchange-inline">be sure </del>to <del class="diffchange diffchange-inline">read </del>the <del class="diffchange diffchange-inline"><a href="https://igem</del>.<del class="diffchange diffchange-inline">org/Safety" style="font</del>-<del class="diffchange diffchange-inline">weight</del>:<del class="diffchange diffchange-inline">bold;"></del>Safety in <del class="diffchange diffchange-inline">iGEM</del><<del class="diffchange diffchange-inline">/</del>a> <del class="diffchange diffchange-inline">page</del>.<<del class="diffchange diffchange-inline">/</del>p></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><p></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">1. The bacterial strains used for research are non-pathogenic (XL-10 Gold Ultra competent Cells and DH5-alpha)</ins>, <ins class="diffchange diffchange-inline">indicating little risk </ins>to the <ins class="diffchange diffchange-inline">research or public</ins>. <ins class="diffchange diffchange-inline">The introduction of manmade biological parts into the environment is an inherent risk, mirroring those of all man</ins>-<ins class="diffchange diffchange-inline">made technologies in biology</ins>: <ins class="diffchange diffchange-inline">the introduction of constructed materials to the detriment of the natural environment.</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">In addition to the bacteria, silicic acid is used to induce the formation of silica matrices on the biofilm surface. Silicic acid has no documented health effects, but its derivative, Silica, has been associated with silicosis, bronchitis, and cancer (Reuzel et. al, “Subchronic inhiliation toxicity of amorphous silicas and quartz dust in rats.” ) The context of these studies are in prolonged exposure above the Occupational Health and </ins>Safety <ins class="diffchange diffchange-inline">Association limit of 0.1mg/m^3 </ins>in <ins class="diffchange diffchange-inline">aersol and dust form. More relevant studies following long-term exposure to silica in water found correlation to dementia when settled with aluminum (Rondeau et. al, “Aluminum and silica in drinking water and the risk of Alzheimer’s disease…”). Because detached biofilm would polymerize added silicic acid into silica, those may pose a potential threat if consumed about OSHA recommended intake levels.</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><<ins class="diffchange diffchange-inline">p></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">2. Because the constructed parts do not contain or produce silica or silica derivatives, are hosted in non-pathogenic strains, and contain no expected selective advantage, they individually pose no potential threat. The parts up-regulate natural expression of the adhesion protein Curli and attach </ins>a <ins class="diffchange diffchange-inline">membrane-spanning protein to the silica-polymerizing enzyme silicatein–alpha. Out of context of biofilm attachment for water purification, the parts pose no threat to the researcher, the public or the environment. Accordingly, no safety concerns were indicated in the parts registry, although future teams may wish to further characterize the silica matrix formation in terms of detachment in water flow in respect to health effects.</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline"><p</ins>></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">3. Purdue University Institutional Biosafety Committee oversees the laboratories working with recombinant DNA technologies under the guidelines of the National Institutes of Health Guidelines for Research Involving Recombinant DNA Molecules, the Centers for Disease Control, and Purdue’s BioSafety Manual. Purdue’s iGEM Team functions under a Biosafety Level I designation, with all biological agents meeting the definition of Risk Group 1 and ‘unknown or minimal potential hazard to laboratory personnel and the environment’. The Purdue IBC regulates the Team and its project as such</ins>.</div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><p></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">4. To ensure biological safety in future iGEM competitions, a program screening sequences of DNA constructs against toxicity databases to alert the presence of a known hazardous element should be developed. This would be an easy and automated ‘first-defense’ for constructed devices.</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline"> </ins></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;"><p><strong>Key questions </strong></p></del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;"><p>For iGEM 2012, teams are asked to detail how they approached any issues of biological safety associated with their projects. Specifically, teams should consider the following questions: </p></del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;"></div></del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;"></del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;"></del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;"><div id="preventing_malign_use"></del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;"></del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;"><table border="0"></del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;"><tr></del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;"><td valign=Top bgcolor="#FFFF99"> </del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;"> <ol></del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;"> <li><strong>Would any of your project ideas raise safety issues in terms of:</del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;"> </strong></del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;"> <ul></del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;"> <li> researcher safety, </li></del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;"> <li> public safety, or </li></del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;"> <li> environmental safety? </li></del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;"> </ul></del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;"> </li></del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;"> <li><strong> Do any of the new BioBrick parts (or devices) that you made this year raise any safety issues? If yes,</del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;"> </strong></del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;"> <ul></del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;"> <li> did you document these issues in the Registry? </li></del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;"> <li> how did you manage to handle the safety issue? </li></del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;"> <li> How could other teams learn from your experience? </li></del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;"> </ul></del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;"> </li></del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;"> <li><strong>Is there a local biosafety group, committee, or review board at your institution? </strong></del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;"> <ul></del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;"> <li> If yes, what does your local biosafety group think about your project? </li></del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;"> <li> If no, which specific biosafety rules or guidelines do you have to consider in your country? </li></del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;"> </ul></del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;"> </li></del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;"> <li><strong>Do you have any other ideas how to deal with safety issues that could be useful for future iGEM competitions? How could parts, devices and systems be made even safer through biosafety engineering? </strong></li></del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;"> </ol></td></del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;"> <td><p>&nbsp;</p> </td></del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;"></tr></del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;"></table></del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p><span class="style1">Teams, please document any answers to these safety questions on your wiki safety page. Judges will be asked to evaluate your project, in part, on the basis of if and how you considered and addressed issues of biological safety.</span> If any questions arise regarding iGEM and biological safety please send an email to safety AT igem.org. </p></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p><span class="style1">Teams, please document any answers to these safety questions on your wiki safety page. Judges will be asked to evaluate your project, in part, on the basis of if and how you considered and addressed issues of biological safety.</span> If any questions arise regarding iGEM and biological safety please send an email to safety AT igem.org. </p></div></td></tr>
</table>Gshowalthttp://2012.igem.org/wiki/index.php?title=Team:Purdue/Safety&diff=43059&oldid=prevCThompson at 19:45, 20 July 20122012-07-20T19:45:32Z<p></p>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>{{<del class="diffchange diffchange-inline">Purdue_topbar</del>}}</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>{{<ins class="diffchange diffchange-inline">Purdue_topbarSafety</ins>}}</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Use this page to answer the questions on the [[Safety | safety page]].</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Use this page to answer the questions on the [[Safety | safety page]].</div></td></tr>
</table>CThompsonhttp://2012.igem.org/wiki/index.php?title=Team:Purdue/Safety&diff=16921&oldid=prevNbalacha at 19:08, 23 June 20122012-06-23T19:08:00Z<p></p>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>{<del class="diffchange diffchange-inline">| style="color:#1b2c8a;background-color:#0c6;" cellpadding="3" cellspacing="1" border="1" bordercolor="#fff" width="62%" align="center"</del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>{<ins class="diffchange diffchange-inline">{Purdue_topbar}</ins>}</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline">!align="center"|[[Team:Purdue|Home]]</del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline">!align="center"|[[Team:Purdue/Team|Team]]</del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline">!align="center"|[https://igem.org/Team.cgi?year=2012&team_name=Purdue Official Team Profile]</del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline">!align="center"|[[Team:Purdue/Project|Project]]</del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline">!align="center"|[[Team:Purdue/Parts|Parts Submitted to the Registry]]</del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline">!align="center"|[[Team:Purdue/Modeling|Modeling]]</del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline">!align="center"|[[Team:Purdue/Notebook|Notebook]]</del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline">!align="center"|[[Team:Purdue/Human Practices|Human Practices]]</del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline">!align="center"|[[Team:Purdue/Safety|Safety]]</del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline">!align="center"|[[Team:Purdue/Attributions|Attributions]]</del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline">!align="center"|[[Team:Purdue/Protocol|Protocol]]</del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline">|</del>}</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div> </div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Use this page to answer the questions on the [[Safety | safety page]].</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Use this page to answer the questions on the [[Safety | safety page]].</div></td></tr>
</table>Nbalacha