Team:Purdue/Parts

From 2012.igem.org

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<h1> Parts Registry </h1>
<h1> Parts Registry </h1>
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<table  class="wikitable sortable" border="0" style="text-align:left >
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<td> Name</td><td>Description</td><td>Registry link</td><td>Part type</td><td>Availability</td></tr>
<td> Name</td><td>Description</td><td>Registry link</td><td>Part type</td><td>Availability</td></tr>
<tr>
<tr>
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<td>precA_LacZ</td><td><img src="https://static.igem.org/mediawiki/2012hs/c/c0/BBa_K862000.PNG"/><br/>
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<td>OmpR234 under TetR Promoter</td><td><img src=""/><br/>
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<br/>
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This is a part for the precise quantification of UV-radiation or radioactive radiation in <i>E.coli</i> (recA+) strains, i.e. BL21(DE3). It consists of a RecA promoter (<a href="http://partsregistry.org/Part:BBa_J22106" class="external text" title="http://partsregistry.org/Part:BBa_J22106 rel="nofollow">BBa_J22106</a>) fused to a LacZ reporter cloned in front of a double terminator (<a href="http://partsregistry.org/Part:BBa_K173004" class="external text" title="http://partsregistry.org/Part:BBa_K173004 rel="nofollow">BBa_K173004</a>).</td><td>
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Adhesion Protein</td><td>
<p>
<p>
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   <a href="http://partsregistry.org/Part:BBa_K862000">BBa_K862000</a>  
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   <a href="http://partsregistry.org/Part:BBa_K932000">BBa_K932000</a>  
  </p>
  </p>
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</td><td> Measurement</td><td><a href="http://partsregistry.org/Main_Page"> parts registry</a></td></tr>
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</td><td>Composite</td><td><a href="http://partsregistry.org/Main_Page">Available</a></td></tr>
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<td>psulA_LacZ</td><td><img src="https://static.igem.org/mediawiki/2012hs/4/4f/BBa_K862001.PNG"/><br/>
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<td>OmpR234</td><td><img src=""/><br/>
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This is a part for the precise quantification of UV-radiation or radioactive radiation in <i>E.coli</i> (recA+) strains, i.e. BL21(DE3). It consists of a SulA promoter (<a href="http://partsregistry.org/Part:BBa_K518010" class="external text" title="http://partsregistry.org/Part:BBa_K518010 rel="nofollow">BBa_K518010</a>) fused to a LacZ reporter cloned in front of a double terminator (<a href="http://partsregistry.org/Part:BBa_K173004" class="external text" title="http://partsregistry.org/Part:BBa_K173004 rel="nofollow">BBa_K173004</a>). <td><a href="http://partsregistry.org/Part:BBa_K862001" class="external text" title="http://partsregistry.org/Part:BBa_K862001 rel="nofollow">BBa_K862001</a></td><td> Measurement
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Transcriptional Activator</td><td>
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<p>
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  <a href="http://partsregistry.org/Part:BBa_K932001">BBa_K932001</a>  
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</p>
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</td><td>Coding</td><td><a href="http://partsregistry.org/Main_Page">N/A</a></td></tr>
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</td><td><a href="http://partsregistry.org/Main_Page"> parts registry</a></td></tr>
 
<tr>
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<td>precB_LacZ</td><td><img src="https://static.igem.org/mediawiki/2012hs/a/a8/BBa_K862002.PNG"/><br/>
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<td>OmpA-Silicatein Alpha Fusion</td><td><img src=""/><br/>
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This is a part for the precise quantification of UV-radiation or radioactive radiation in <i>E. coli</i> (recA+) strains, i.e. BL21(DE3). It consists of a RecB promoter (<a href="http://partsregistry.org/Part:BBa_K862003" class="external text" title="http://partsregistry.org/Part:BBa_K862003 rel="nofollow">BBa_K862003</a>) fused to a LacZ reporter cloned in front of a double terminator (<a href="http://partsregistry.org/Part:BBa_K173004" class="external text" title="http://partsregistry.org/Part:BBa_K173004 rel="nofollow">BBa_K173004</a>).</td><td><a href="http://partsregistry.org/Part:BBa_K862002" class="external text" title="http://partsregistry.org/Part:BBa_K862002" rel="nofollow">BBa_K862002</a></td><td> Measurement
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Outer Membrane Silica Binding Protein</td><td>
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<p>
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  <a href="http://partsregistry.org/Part:BBa_K932002">BBa_K932002</a>  
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</p>
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</td><td>Coding</td><td><a href="http://partsregistry.org/Main_Page">N/A</a></td></tr>
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</td><td><a href="http://partsregistry.org/Main_Page"> parts registry</a></td></tr>
 
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</td></tr>
 
<tr>
<tr>
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<td>precB</td><td><img src="http://partsregistry.org/images/partbypart/icon_regulatory.png"/><br/>
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<td>OmpA-Sila and Tet Repressor under Lac Promoter</td><td><img src=""/><br/>
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The RecB promoter sequence was obtained from the <i>E.coli</i> MG1655 genome sequence (<a href="http://ecoliwiki.net/colipedia/index.php/recB:Gene">http://ecoliwiki.net/colipedia/index.php/recB:Gene</a>). We assumed the main promoter region to be located from -70 to -1 bp upstream the recB start codon. Therefore this sequence was synthesized and cloned on an oligo basis.
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OmpA-SilA under Lac Promoter</td><td>
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We will not submit the physical DNA of this part, but we provide the oligo-sequences you can use for synthesizing and cloning this part in front of any EcoRI/XbaI predigested reporter part.
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<br>
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<p>
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<br/>RecB_fw: 5'-aattcgcggccgcttctagagCCTGAAGGCTGGAAAGTGTGGGAGAA</br>CGTCAGCGCGTTGCAGCAAACAATGCCCCTGATGAGTGAAAAGAc-3' <br>
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  <a href="http://partsregistry.org/Part:BBa_K932003">BBa_K932003</a>  
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<br>RecB_rev: 5'-ctaggTCTTTTCACTCATCAGGGGCATTGTTTGCTGCAAC</br>GCGCTGACGTTCTCCCACACTTTCCAGCCTTCAGGctctagaagcggccgcg-3'</td><td><a href="http://partsregistry.org/Part:BBa_K862003" class="external text" title="http://partsregistry.org/Part:BBa_K862003" rel="nofollow">BBa_K862003</a></td><td> Regulatory
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</p>
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</td><td>Composite</td><td><a href="http://partsregistry.org/Main_Page">Unavailable</a></td></tr>
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</td><td>can be cloned on oligo basis (sequence provided)</td></tr>
 
</table>
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Latest revision as of 00:55, 16 October 2012

Parts Registry

NameDescriptionRegistry linkPart typeAvailability
OmpR234 under TetR Promoter

Adhesion Protein

BBa_K932000

CompositeAvailable
OmpR234

Transcriptional Activator

BBa_K932001

CodingN/A
OmpA-Silicatein Alpha Fusion

Outer Membrane Silica Binding Protein

BBa_K932002

CodingN/A
OmpA-Sila and Tet Repressor under Lac Promoter

OmpA-SilA under Lac Promoter

BBa_K932003

CompositeUnavailable