Team:Purdue/Parts

From 2012.igem.org

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</td><td> Measurement</td><td><a href="http://partsregistry.org/Main_Page"> parts registry</a></td></tr>
</td><td> Measurement</td><td><a href="http://partsregistry.org/Main_Page"> parts registry</a></td></tr>
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<td>psulA_LacZ</td><td><img src="https://static.igem.org/mediawiki/2012hs/4/4f/BBa_K862001.PNG"/><br/>
 
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This is a part for the precise quantification of UV-radiation or radioactive radiation in <i>E.coli</i> (recA+) strains, i.e. BL21(DE3). It consists of a SulA promoter (<a href="http://partsregistry.org/Part:BBa_K518010" class="external text" title="http://partsregistry.org/Part:BBa_K518010 rel="nofollow">BBa_K518010</a>) fused to a LacZ reporter cloned in front of a double terminator (<a href="http://partsregistry.org/Part:BBa_K173004" class="external text" title="http://partsregistry.org/Part:BBa_K173004 rel="nofollow">BBa_K173004</a>). <td><a href="http://partsregistry.org/Part:BBa_K862001" class="external text" title="http://partsregistry.org/Part:BBa_K862001 rel="nofollow">BBa_K862001</a></td><td> Measurement
 
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</td><td><a href="http://partsregistry.org/Main_Page"> parts registry</a></td></tr>
 
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<td>precB_LacZ</td><td><img src="https://static.igem.org/mediawiki/2012hs/a/a8/BBa_K862002.PNG"/><br/>
 
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This is a part for the precise quantification of UV-radiation or radioactive radiation in <i>E. coli</i> (recA+) strains, i.e. BL21(DE3). It consists of a RecB promoter (<a href="http://partsregistry.org/Part:BBa_K862003" class="external text" title="http://partsregistry.org/Part:BBa_K862003 rel="nofollow">BBa_K862003</a>) fused to a LacZ reporter cloned in front of a double terminator (<a href="http://partsregistry.org/Part:BBa_K173004" class="external text" title="http://partsregistry.org/Part:BBa_K173004 rel="nofollow">BBa_K173004</a>).</td><td><a href="http://partsregistry.org/Part:BBa_K862002" class="external text" title="http://partsregistry.org/Part:BBa_K862002" rel="nofollow">BBa_K862002</a></td><td> Measurement
 
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</td><td><a href="http://partsregistry.org/Main_Page"> parts registry</a></td></tr>
 
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<td>precB</td><td><img src="http://partsregistry.org/images/partbypart/icon_regulatory.png"/><br/>
 
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The RecB promoter sequence was obtained from the <i>E.coli</i> MG1655 genome sequence (<a href="http://ecoliwiki.net/colipedia/index.php/recB:Gene">http://ecoliwiki.net/colipedia/index.php/recB:Gene</a>). We assumed the main promoter region to be located from -70 to -1 bp upstream the recB start codon. Therefore this sequence was synthesized and cloned on an oligo basis.
 
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We will not submit the physical DNA of this part, but we provide the oligo-sequences you can use for synthesizing and cloning this part in front of any EcoRI/XbaI predigested reporter part.
 
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<br/>RecB_fw: 5'-aattcgcggccgcttctagagCCTGAAGGCTGGAAAGTGTGGGAGAA</br>CGTCAGCGCGTTGCAGCAAACAATGCCCCTGATGAGTGAAAAGAc-3' <br>
 
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<br>RecB_rev: 5'-ctaggTCTTTTCACTCATCAGGGGCATTGTTTGCTGCAAC</br>GCGCTGACGTTCTCCCACACTTTCCAGCCTTCAGGctctagaagcggccgcg-3'</td><td><a href="http://partsregistry.org/Part:BBa_K862003" class="external text" title="http://partsregistry.org/Part:BBa_K862003" rel="nofollow">BBa_K862003</a></td><td> Regulatory
 
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</td><td>can be cloned on oligo basis (sequence provided)</td></tr>
 
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Revision as of 02:45, 7 July 2012

Parts Registry

NameDescriptionRegistry linkPart typeAvailability
Part 1

Description

Link

Measurement parts registry

Retrieved from "http://2012.igem.org/Team:Purdue/Parts"