"
Team:Potsdam Bioware/Lab/Labjournal/October
From 2012.igem.org
(→2012-10-22) |
(→2012-10-23) |
||
| Line 130: | Line 130: | ||
<b>Topic: </b>cell-culture <br> | <b>Topic: </b>cell-culture <br> | ||
<br> | <br> | ||
| - | * | + | * seeding of CHO and HeLa in Ibidi Dishes for transfection with new Nanobody RAGE construct |
| - | * | + | * infection with Virus (CFP on surface and YFP as GOI, AAV with Sortase motif) |
| - | + | ||
| - | + | ||
| - | + | ||
===<p style="background-color: rgb(240, 20, 70);">2012-10-24</p>=== | ===<p style="background-color: rgb(240, 20, 70);">2012-10-24</p>=== | ||
Revision as of 13:53, 25 October 2012
Contents |
AID
2012-10-10
Inoculation of plasmid samples of the 48h retransformation plates (after FACS)
Investigators: Tom
Time: 2012-10-10
Materials:
- LB medium
- Amp stock solution
- plate with cultures:
EGFR-C-AID without NES, with NLS+Kozak sequence+eGFP
(after FACS selection)
Method:
Inoculation of:
5 cultures per plate in 5 ml LB medium + 5µL Amp.
(--> 5 cultures)
Further tasks:
- Miniprep
Antibody
2012-10-12
cell culture
Investigator:Kerstin
Topic: cell-culture
- passaging of CHO w Zeocin
- passaging of HT1080
- passaging of HEK AAV 293
- passaging of HeLa
2012-10-15
cell culture
Investigator:Kerstin
Topic: cell-culture
- passaging of CHO w Zeocin
- passaging of HT1080
- passaging of HEK AAV 293
- passaging of HeLa
2012-10-17
cell culture
Investigator:Kerstin
Topic: cell-culture
- passaging of CHO w Zeocin
- passaging of HT1080
- passaging of HEK AAV 293
- passaging of HeLa
- passaging of stably transfected Clones 4.1, 4.2, 4.3, 4,4, 29.1, 29.2, 29.3 with 550µg/ml Hygromycin
2012-10-19
cell culture
Investigator:Kerstin
Topic: cell-culture
- passaging of CHO w Zeocin
- passaging of HT1080
- passaging of HEK AAV 293
- passaging of HeLa
2012-10-22
cell culture
Investigator:Kerstin
Topic: cell-culture
- passaging of CHO w Zeocin
- passaging of HT1080
- passaging of HEK AAV 293
- passaging of HeLa
- transfection of stably transfected clone 29.1 with Cre Recombinase
- seeding of HT1080 in Ibidi Dish for infection with virus
2012-10-23
cell culture
Investigator:Kerstin
Topic: cell-culture
- seeding of CHO and HeLa in Ibidi Dishes for transfection with new Nanobody RAGE construct
- infection with Virus (CFP on surface and YFP as GOI, AAV with Sortase motif)
2012-10-24
cell culture
Investigator:Kerstin
Topic: cell-culture
- passaging of CHO w Zeocin
- passaging of HT1080
- passaging of HEK AAV 293
- passaging of HeLa
2012-10-25
cell culture
Investigator:Kerstin
Topic: cell-culture
- passaging of CHO w Zeocin
- passaging of HT1080
- passaging of HEK AAV 293
- passaging of HeLa
Virus
XXX
XXX
Investigator: XXX
Aim: XXX
Materials:
XXX
Method:
XXX
Results:
XXX
Further tasks
XXX
