BBa_929107
Anti-GFP, IgG1 Fc with TEV site, TMR, mCherry, framed by LoxP
Antibody Module
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This part is an improved version of wildtype AID, the enzyme that randomly mutates predominantly in the immunoglobulin genes. It is designed for strong expression of the fusion protein modified AID+eGFP. Modified AID has an additional Nuclear Localization Sequence (NLS)and the naturally occurring Nuclear Export Sequence (NES)is deleted. Due to the fact that AID mutates the actively transcribed single stranded DNA, it is supposed that the direction of the enzyme to the inside of the nucleus would improve the mutation rate.The fusion with the green fluorescent reporter eGFP allows 1.)to check the transfection success 2.)select transfected cells via FACS and 3.)to check the cellular localization of the fusion protein. For detailed information see the partsregistry, for experimental experience also see All Biobricks.
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BBa_K929003
modified AID with CMV, hGH-polyA and eGFP
Mutation Module
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This part is an improved version of wildtype AID, the enzyme that randomly mutates predominantly in the immunoglobulin genes. It is designed for strong expression of the fusion protein modified AID+eGFP. Modified AID has an additional Nuclear Localization Sequence (NLS)and the naturally occurring Nuclear Export Sequence (NES)is deleted. Due to the fact that AID mutates the actively transcribed single stranded DNA, it is supposed that the direction of the enzyme to the inside of the nucleus would improve the mutation rate.The fusion with the green fluorescent reporter eGFP allows 1.)to check the transfection success 2.)select transfected cells via FACS and 3.)to check the cellular localization of the fusion protein. For detailed information see the partsregistry, for experimental experience also see All Biobricks.
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BBa_K929301
Potsdam Standard Backbone
Potsdam Standard
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This part is an improved version of wildtype AID, the enzyme that randomly mutates predominantly in the immunoglobulin genes. It is designed for strong expression of the fusion protein modified AID+eGFP. Modified AID has an additional Nuclear Localization Sequence (NLS)and the naturally occurring Nuclear Export Sequence (NES)is deleted. Due to the fact that AID mutates the actively transcribed single stranded DNA, it is supposed that the direction of the enzyme to the inside of the nucleus would improve the mutation rate.The fusion with the green fluorescent reporter eGFP allows 1.)to check the transfection success 2.)select transfected cells via FACS and 3.)to check the cellular localization of the fusion protein. For detailed information see the partsregistry, for experimental experience also see All Biobricks.
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