Team:Potsdam Bioware/Biobricks/Overview

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Biobricks

Overview

UP12biobricks.png


[http://syntbio.net/iGEM/wiki2011/index.php/TESTWIKI#antibody_module_parts Selection Parts]

[http://syntbio.net/iGEM/wiki2011/index.php/TESTWIKI#antibody_module_parts Antibody Parts]

[http://syntbio.net/iGEM/wiki2011/index.php/TESTWIKI#antibody_module_parts Mutation Parts]


Favorite Biobricks

[http://partsregistry.org/Part:BBa_K929003 BBa_K929003]

modified AID with CMV, hGH-polyA and eGFP
Mutation Module

UP12 BBa K929003 smaloverview.png
It was designed for strong expression of the fusion protein modified AID+eGFP. In comparison to the part the green fluorescent reporter eGFP allows 1.)to check the transfection success 2.)sort transfected cells via FACS and 3.)to check the cellular localization of the fusion protein.