Team:Potsdam Bioware/Biobricks/Overview
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Biobricks
Overview
[http://syntbio.net/iGEM/wiki2011/index.php/TESTWIKI#antibody_module_parts Selection Parts]
[http://syntbio.net/iGEM/wiki2011/index.php/TESTWIKI#antibody_module_parts Antibody Parts]
[http://syntbio.net/iGEM/wiki2011/index.php/TESTWIKI#antibody_module_parts Mutation Parts]
Favorite Biobricks
[http://partsregistry.org/Part:BBa_K929003 BBa_K929003] modified AID with CMV, hGH-polyA and eGFP |
It was designed for strong expression of the fusion protein modified AID+eGFP. In comparison to the part the green fluorescent reporter eGFP allows 1.)to check the transfection success 2.)sort transfected cells via FACS and 3.)to check the cellular localization of the fusion protein. | |