Team:Potsdam Bioware/Biobricks/Overview

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Revision as of 17:59, 26 September 2012

Biobricks

Overview

Selection Parts

Antibody Parts

Mutation Parts

Favorite Biobricks

BBa_K929003 modified AID with CMV, hGH-polyA and eGFP Mutation Module	This part is an improved version of wildtype AID, the enzyme that randomly mutates predominantly in the immunoglobulin genes. It is designed for strong expression of the fusion protein modified AID+eGFP. Modified AID has an additional Nuclear Localization Sequence (NLS)and the naturally occurring Nuclear Export Sequence (NES)is deleted. Due to the fact that AID mutates the actively transcribed single stranded DNA, it is supposed that the direction of the enzyme to the inside of the nucleus would improve the mutation rate.The fusion with the green fluorescent reporter eGFP allows 1.)to check the transfection success 2.)sort transfected cells via FACS and 3.)to check the cellular localization of the fusion protein. For detailed information see the partsregistry, for experimental experience also see All Biobricks.

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 Mutation Module
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-    <td> [[Image:UP12_BBa_K929003_smaloverview.png|right|400px]] This part is an improved version of wildtype AID, the enzyme that randomly mutates predominantly in the immunoglobulin genes. It is designed for strong expression of the fusion protein modified AID+eGFP. Modified AID has an additional Nuclear Localization Sequence (NLS)and the naturally occurring Nuclear Export Sequence (NES)is deleted. Due to the fact that AID mutates the actively transcribed single stranded DNA, it is supposed that the direction of the enzyme to the inside of the nucleus would improve the mutation rate.The fusion with the green fluorescent reporter eGFP allows 1.)to check the transfection success 2.)sort transfected cells via FACS and 3.)to check the cellular localization of the fusion protein. For detailed information see the [partsregistry], for experimental experience also see All Biobricks.
+    <td> [[Image:UP12_BBa_K929003_smaloverview.png|right|400px]] This part is an improved version of wildtype AID, the enzyme that randomly mutates predominantly in the immunoglobulin genes. It is designed for strong expression of the fusion protein modified AID+eGFP. Modified AID has an additional Nuclear Localization Sequence (NLS)and the naturally occurring Nuclear Export Sequence (NES)is deleted. Due to the fact that AID mutates the actively transcribed single stranded DNA, it is supposed that the direction of the enzyme to the inside of the nucleus would improve the mutation rate.The fusion with the green fluorescent reporter eGFP allows 1.)to check the transfection success 2.)sort transfected cells via FACS and 3.)to check the cellular localization of the fusion protein. For detailed information see the [http://partsregistry.org/Part:BBa_K929003 partsregistry], for experimental experience also see All Biobricks.