Team:Potsdam Bioware/Biobricks/Overview

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   <td>[http://partsregistry.org/Part:BBa_K929003 BBa_K929003]<br>
   <td>[http://partsregistry.org/Part:BBa_K929003 BBa_K929003]<br>
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modified AID with CMV, hGH-polyA and eGFP<br>
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'''modified AID with CMV, hGH-polyA and eGFP'''<br>
Mutation Module
Mutation Module
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Revision as of 17:57, 26 September 2012


Biobricks

Overview

UP12biobricks.png


Selection Parts

Antibody Parts

Mutation Parts


Favorite Biobricks

BBa_K929003

modified AID with CMV, hGH-polyA and eGFP
Mutation Module

UP12 BBa K929003 smaloverview.png
This part is an improved version of wildtype AID, the enzyme that randomly mutates predominantly in the immunoglobulin genes. It is designed for strong expression of the fusion protein modified AID+eGFP. Modified AID has an additional Nuclear Localization Sequence (NLS)and the naturally occurring Nuclear Export Sequence (NES)is deleted. Due to the fact that AID mutates the actively transcribed single stranded DNA, it is supposed that the direction of the enzyme to the inside of the nucleus would improve the mutation rate.The fusion with the green fluorescent reporter eGFP allows 1.)to check the transfection success 2.)sort transfected cells via FACS and 3.)to check the cellular localization of the fusion protein. For detailed information see the [partsregistry], for experimental experience also see All Biobricks.




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