Team:Potsdam Bioware/Biobricks/Overview

From 2012.igem.org

(Difference between revisions)

Revision as of 21:45, 26 September 2012

Biobricks

Overview

[http://syntbio.net/iGEM/wiki2011/index.php/TESTWIKI#antibody_module_parts Selection Parts]

[http://syntbio.net/iGEM/wiki2011/index.php/TESTWIKI#antibody_module_parts Antibody Parts]

[http://syntbio.net/iGEM/wiki2011/index.php/TESTWIKI#antibody_module_parts Mutation Parts]

Favorite Biobricks

[http://partsregistry.org/Part:BBa_929107 BBa_929107] Anti-GFP, IgG1 Fc with TEV site, TMR, mCherry, framed by LoxP Antibody Module	This part is an improved version of wildtype AID, the enzyme that randomly mutates predominantly in the immunoglobulin genes. It is designed for strong expression of the fusion protein modified AID+eGFP. Modified AID has an additional Nuclear Localization Sequence (NLS)and the naturally occurring Nuclear Export Sequence (NES)is deleted. Due to the fact that AID mutates the actively transcribed single stranded DNA, it is supposed that the direction of the enzyme to the inside of the nucleus would improve the mutation rate.The fusion with the green fluorescent reporter eGFP allows 1.)to check the transfection success 2.)select transfected cells via FACS and 3.)to check the cellular localization of the fusion protein. For detailed information see the [http://partsregistry.org/Part:BBa_K929003 partsregistry], for experimental experience also see All Biobricks.
[http://partsregistry.org/Part:BBa_K929003 BBa_K929003] modified AID with CMV, hGH-polyA and eGFP Mutation Module	This part is an improved version of wildtype AID, the enzyme that randomly mutates predominantly in the immunoglobulin genes. It is designed for strong expression of the fusion protein modified AID+eGFP. Modified AID has an additional Nuclear Localization Sequence (NLS)and the naturally occurring Nuclear Export Sequence (NES)is deleted. Due to the fact that AID mutates the actively transcribed single stranded DNA, it is supposed that the direction of the enzyme to the inside of the nucleus would improve the mutation rate.The fusion with the green fluorescent reporter eGFP allows 1.)to check the transfection success 2.)select transfected cells via FACS and 3.)to check the cellular localization of the fusion protein. For detailed information see the [http://partsregistry.org/Part:BBa_K929003 partsregistry], for experimental experience also see All Biobricks.
[http://partsregistry.org/Part:BBa_K929301 BBa_K929301] Potsdam Standard Backbone Potsdam Standard	This part is an improved version of wildtype AID, the enzyme that randomly mutates predominantly in the immunoglobulin genes. It is designed for strong expression of the fusion protein modified AID+eGFP. Modified AID has an additional Nuclear Localization Sequence (NLS)and the naturally occurring Nuclear Export Sequence (NES)is deleted. Due to the fact that AID mutates the actively transcribed single stranded DNA, it is supposed that the direction of the enzyme to the inside of the nucleus would improve the mutation rate.The fusion with the green fluorescent reporter eGFP allows 1.)to check the transfection success 2.)select transfected cells via FACS and 3.)to check the cellular localization of the fusion protein. For detailed information see the [http://partsregistry.org/Part:BBa_K929003 partsregistry], for experimental experience also see All Biobricks.

@@ Line 7: / Line 7: @@
 [[File:UP12biobricks.png|600px|left]]
 <html>
-<img src="UP12biobricks.png" border=0  height="60">