Team:Potsdam Bioware/Biobricks/All Biobricks

All Biobricks

As the project is divided into three subproject, the resulting biobricks are listet in the three categories. Furthermore the Biobricks for the new RFC "Potsdam Standard" are listed.

Antibody Biobricks

Mutation Biobricks

All the Mutation Biobricks are improved/modified versions of the wildtype AID that was available as a Biobrick (BBa_K103001). AID is known to be responsible for somatic hypermutation and the class-switch recombination of immunoglobulin in B cells. This enzyme of 28 kDa originally occurs in B cells but does also show activity after transfection into CHO cells. AID induces the deamination of cytidine to uridine at actively transcribed single strand DNA. The replacement of cytidine by uridine leads to a mismatch during DNA replication and integrates a single base substitution predominantly in the immunoglobulin genesWith PCR and cloning we developed AID`s for the differnt requirements.ID is known to be responsible for somatic hypermutation and the class-switch recombination of immunoglobulin in B cells. This enzyme of 28 kDa originally occurs in B cells but does also show activity after transfection into CHO cells. AID induces the deamination of cytidine to uridine at actively transcribed single strand DNA. The replacement of cytidine by uridine leads to a mismatch during DNA replication and integrates a single base substitution predominantly in the immunoglobulin genes.

The AID motif is naturally terminated with the Nuclear Export Sequence (NES) that causes the protein to translocate from the nucleus to the cytoplasm. Additionally, upstream, dysfunctional Nuclear Localization Sequence (NLS) is located. Due to the fact that AID mutates the actively transcribed single stranded DNA, it is supposed that the direction of the enzyme to the inside of the nucleus would improve the mutation rate.

Virus Biobricks

Potsdam Standard Biobricks