Team:Penn State/Parts

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Revision as of 21:31, 22 October 2012

Index

Parts Submitted to the Registry

Check out the parts we have submitted to the Registry of Standard Biological Parts! This year's parts include two of our main project constructs, as well as one successful test construct. Be on the lookout for many more to come!

Registry of Biological Parts

Name Type Description Symbolic Model Length (bp)
BBa_K933000 device multiple start codons coding for RFP and sfGFP respectively design  
BBa_K933001 device repeating threonine 6-codon sequence with mCherry and GFP reporters design  
BBa_K933002 device AHL leader with mCherry and GFP reporters design  

Continuing Research


Codon Optimization

Sequence Name Status Construct Sequenced
theonine 9-repeat codon_1 oligos annealed    
theonine 9-repeat codon_2 oligos annealed    
theonine 9-repeat codon_3 oligos annealed    
theonine 9-repeat codon_alternating oligos annealed    
alanine 9-repeat codon_1 oligos annealed    
alanine 9-repeat codon_2 oligos annealed    
alanine 9-repeat codon_3 oligos annealed    
alanine 9-repeat codon_alternating oligos annealed    
theonine 6-repeat codon_1 complete yes  
theonine 6-repeat codon_2 complete yes  
theonine 6-repeat codon_3 complete    
theonine 6-repeat codon_alternating complete    
alanine 6-repeat codon_1 oligos annealed    
alanine 6-repeat codon_2 oligos annealed    
alanine 6-repeat codon_3 oligos annealed    
alanine 6-repeat codon_alternating oligos annealed    
threonine 3-repeat codon_1 oligos annealed    
threonine 3-repeat codon_2 oligos annealed    
threonine 3-repeat codon_3 oligos annealed    
threonine 3-repeat codon_alternating oligos annealed    

Bidirectional Promoters

Sequence Name Status Part Sequenced
BD1 complete yes
BD2 complete yes
BD3 complete yes
BBa_J23102 complete yes
BBa_J23103 oligos annealed  
BBa_J23114 oligos annealed  

Multiple Start Codons

Sequence Name Status Part Sequenced
RBS1strong_RBS2weak small length oligos annealed  
RBS1weak_RBS2strong small length oligos annealed  
RBS1strong_RBS2strong small length oligos annealed  
RBS1strong_RBS2weak med length oligos annealed  
RBS1weak_RBS2strong med length complete yes
RBS1strong_RBS2strong med length order oligos  
RBS1strong_RBS2weak med length oligos annealed  
RBS1weak_RBS2strong med length oligos annealed  
RBS1strong_RBS2strong med length order oligos