Team:Penn State/Parts

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<a class="activenavitab" href="http://2012.igem.org/Team:Penn_State/Parts">Parts</a><span class="hide"> | </span>
<a class="activenavitab" href="http://2012.igem.org/Team:Penn_State/Parts">Parts</a><span class="hide"> | </span>
-
<a class="navitab" href="http://2012.igem.org/Team:Penn_State/Modeling">Modeling</a><span class="hide"> | </span>
+
<a class="navitab" href="http://2012.igem.org/Team:Penn_State/Modeling">Main Results</a><span class="hide"> | </span>
<a class="navitab" href="http://2012.igem.org/Team:Penn_State/Notebook">Notebook</a><span class="hide"> | </span>
<a class="navitab" href="http://2012.igem.org/Team:Penn_State/Notebook">Notebook</a><span class="hide"> | </span>
<a class="navitab" href="http://2012.igem.org/Team:Penn_State/Other">Other</a>
<a class="navitab" href="http://2012.igem.org/Team:Penn_State/Other">Other</a>
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     <th scope="col">Sequence Name</th>
     <th scope="col">Sequence Name</th>
     <th scope="col">Status</th>
     <th scope="col">Status</th>
-
    <th scope="col">Construct Sequenced</th>
+
 
      
      
   <tr>
   <tr>
     <td>theonine 9-repeat codon_1</td>
     <td>theonine 9-repeat codon_1</td>
     <td>oligos annealed</td>
     <td>oligos annealed</td>
-
    <td>&nbsp;</td>
 
   </tr>
   </tr>
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     <td>theonine 9-repeat codon_2</td>
     <td>theonine 9-repeat codon_2</td>
     <td>oligos annealed</td>
     <td>oligos annealed</td>
-
    <td>&nbsp;</td>
+
 
   </tr>
   </tr>
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     <td>theonine 9-repeat codon_3</td>
     <td>theonine 9-repeat codon_3</td>
     <td>oligos annealed</td>
     <td>oligos annealed</td>
-
    <td>&nbsp;</td>
+
 
   </tr>
   </tr>
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     <td>theonine 9-repeat codon_alternating</td>
     <td>theonine 9-repeat codon_alternating</td>
     <td>oligos annealed</td>
     <td>oligos annealed</td>
-
    <td>&nbsp;</td>
+
 
   </tr>
   </tr>
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     <td>alanine 9-repeat codon_1</td>
     <td>alanine 9-repeat codon_1</td>
     <td>oligos annealed</td>
     <td>oligos annealed</td>
-
    <td>&nbsp;</td>
+
   </tr>
   </tr>
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     <td>alanine 9-repeat codon_2</td>
     <td>alanine 9-repeat codon_2</td>
     <td>oligos annealed</td>
     <td>oligos annealed</td>
-
    <td>&nbsp;</td>
 
   </tr>
   </tr>
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     <td>alanine 9-repeat codon_3</td>
     <td>alanine 9-repeat codon_3</td>
     <td>oligos annealed</td>
     <td>oligos annealed</td>
-
    <td>&nbsp;</td>
 
   </tr>
   </tr>
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     <td>alanine 9-repeat codon_alternating</td>
     <td>alanine 9-repeat codon_alternating</td>
     <td>oligos annealed</td>
     <td>oligos annealed</td>
-
    <td>&nbsp;</td>
+
 
   </tr>
   </tr>
   <tr>
   <tr>
     <td>theonine 6-repeat codon_1</td>
     <td>theonine 6-repeat codon_1</td>
-
     <td>complete</td>
+
     <td>complete and sequenced</td>
-
    <td>yes</td>
+
 
   </tr>
   </tr>
   <tr>
   <tr>
     <td>theonine 6-repeat codon_2</td>
     <td>theonine 6-repeat codon_2</td>
-
     <td>complete</td>
+
     <td>complete and sequenced</td>
     <td>yes</td>
     <td>yes</td>
-
 
   </tr>
   </tr>
   <tr>
   <tr>
     <td>theonine 6-repeat codon_3</td>
     <td>theonine 6-repeat codon_3</td>
     <td>complete</td>
     <td>complete</td>
-
    <td>&nbsp;</td>
+
 
   </tr>
   </tr>
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     <td>theonine 6-repeat codon_alternating</td>
     <td>theonine 6-repeat codon_alternating</td>
     <td>complete</td>
     <td>complete</td>
-
    <td>&nbsp;</td>
+
 
   </tr>
   </tr>
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     <td>alanine 6-repeat codon_1</td>
     <td>alanine 6-repeat codon_1</td>
     <td>oligos annealed</td>
     <td>oligos annealed</td>
-
    <td>&nbsp;</td>
+
 
   </tr>
   </tr>
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     <td>alanine 6-repeat codon_2</td>
     <td>alanine 6-repeat codon_2</td>
     <td>oligos annealed</td>
     <td>oligos annealed</td>
-
    <td>&nbsp;</td>
+
 
   </tr>
   </tr>
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     <td>alanine 6-repeat codon_3</td>
     <td>alanine 6-repeat codon_3</td>
     <td>oligos annealed</td>
     <td>oligos annealed</td>
-
    <td>&nbsp;</td>
+
 
   </tr>
   </tr>
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     <td>alanine 6-repeat codon_alternating</td>
     <td>alanine 6-repeat codon_alternating</td>
     <td>oligos annealed</td>
     <td>oligos annealed</td>
-
    <td>&nbsp;</td>
+
 
   </tr>
   </tr>
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     <td>threonine 3-repeat codon_1</td>
     <td>threonine 3-repeat codon_1</td>
     <td>oligos annealed</td>
     <td>oligos annealed</td>
-
    <td>&nbsp;</td>
+
 
   </tr>
   </tr>
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     <td>threonine 3-repeat codon_2</td>
     <td>threonine 3-repeat codon_2</td>
     <td>oligos annealed</td>
     <td>oligos annealed</td>
-
    <td>&nbsp;</td>
+
 
   </tr>
   </tr>
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     <td>threonine 3-repeat codon_3</td>
     <td>threonine 3-repeat codon_3</td>
     <td>oligos annealed</td>
     <td>oligos annealed</td>
-
    <td>&nbsp;</td>
+
 
   </tr>
   </tr>
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   </tr>
   </tr>
   <tr>
   <tr>
-
     <td>BD1</td>
+
     <td><a href="http://partsregistry.org/Part:BBa_K933004">BBa_K933004</a></td>
     <td>complete</td>
     <td>complete</td>
     <td>yes</td>
     <td>yes</td>
   </tr>
   </tr>
   <tr>
   <tr>
-
     <td>BD2</td>
+
     <td><a href="http://partsregistry.org/Part:BBa_K933005">BBa_K933005</a></td>
-
     <td>complete</td>
+
     <td>complete and added to Registry</td>
     <td>yes</td>
     <td>yes</td>
   </tr>
   </tr>
   <tr>
   <tr>
-
     <td>BD3</td>
+
     <td><a href="http://partsregistry.org/Part:BBa_K933006">BBa_K933006</a></td>
-
     <td>complete</td>
+
     <td>complete and added to Registry</td>
     <td>yes</td>
     <td>yes</td>
   </tr>
   </tr>
   <tr>
   <tr>
-
     <td>BBa_J23102</td>
+
     <td><a href="http://partsregistry.org/Part:BBa_K933003">BBa_K933003</a></td>
-
     <td>complete</td>
+
     <td>complete and added to Registry</td>
     <td>yes</td>
     <td>yes</td>
   </tr>
   </tr>
   <tr>
   <tr>
-
     <td>BBa_J23103</td>
+
     <td>testing promoter BBa_J23103</td>
     <td>oligos annealed</td>
     <td>oligos annealed</td>
     <td>&nbsp;</td>
     <td>&nbsp;</td>
   </tr>
   </tr>
   <tr>
   <tr>
-
     <td>BBa_J23114</td>
+
     <td>testing promoter BBa_J23114</td>
     <td>oligos annealed</td>
     <td>oligos annealed</td>
     <td>&nbsp;</td>
     <td>&nbsp;</td>
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     <th scope="col">Sequence Name</th>
     <th scope="col">Sequence Name</th>
     <th scope="col">Status</th>
     <th scope="col">Status</th>
-
    <th scope="col">Part Sequenced</th>
+
 
   </tr>
   </tr>
   <tr>
   <tr>
     <td>RBS1strong_RBS2weak small length</td>
     <td>RBS1strong_RBS2weak small length</td>
     <td>oligos annealed</td>
     <td>oligos annealed</td>
-
    <td>&nbsp;</td>
+
 
   </tr>
   </tr>
   <tr>
   <tr>
     <td>RBS1weak_RBS2strong small length</td>
     <td>RBS1weak_RBS2strong small length</td>
     <td>oligos annealed</td>
     <td>oligos annealed</td>
-
    <td>&nbsp;</td>
+
 
   </tr>
   </tr>
   <tr>
   <tr>
     <td>RBS1strong_RBS2strong small length</td>
     <td>RBS1strong_RBS2strong small length</td>
     <td>oligos annealed</td>
     <td>oligos annealed</td>
-
    <td>&nbsp;</td>
+
 
   </tr>
   </tr>
   <tr>
   <tr>
     <td>RBS1strong_RBS2weak med length</td>
     <td>RBS1strong_RBS2weak med length</td>
     <td>oligos annealed</td>
     <td>oligos annealed</td>
-
    <td>&nbsp;</td>
+
 
   </tr>
   </tr>
   <tr>
   <tr>
     <td>RBS1weak_RBS2strong med length</td>
     <td>RBS1weak_RBS2strong med length</td>
-
     <td>complete</td>
+
     <td>complete and sequenced</td>
-
    <td>yes</td>
+
 
   </tr>
   </tr>
   <tr>
   <tr>
     <td>RBS1strong_RBS2strong med length</td>
     <td>RBS1strong_RBS2strong med length</td>
     <td>order oligos</td>
     <td>order oligos</td>
-
    <td>&nbsp;</td>
+
 
   </tr>
   </tr>
   <tr>
   <tr>
     <td>RBS1strong_RBS2weak med length</td>
     <td>RBS1strong_RBS2weak med length</td>
     <td>oligos annealed</td>
     <td>oligos annealed</td>
-
    <td>&nbsp;</td>
+
 
   </tr>
   </tr>
   <tr>
   <tr>
     <td>RBS1weak_RBS2strong med length</td>
     <td>RBS1weak_RBS2strong med length</td>
     <td>oligos annealed</td>
     <td>oligos annealed</td>
-
    <td>&nbsp;</td>
+
 
   </tr>
   </tr>
   <tr>
   <tr>
     <td>RBS1strong_RBS2strong med length</td>
     <td>RBS1strong_RBS2strong med length</td>
     <td>order oligos</td>
     <td>order oligos</td>
-
    <td>&nbsp;</td>
+
 
   </tr>
   </tr>
</table>
</table>

Latest revision as of 03:01, 27 October 2012

Index

Parts Submitted to the Registry

Check out the parts we have submitted to the Registry of Standard Biological Parts! This year's parts include two of our main project constructs, as well as one successful test construct. Be on the lookout for many more to come!

Registry of Biological Parts

Submitted Parts

Name Type Description Symbolic Model Length (bp)
BBa_K933000 device multiple start codons coding for RFP and sfGFP respectively design  
BBa_K933001 device repeating threonine 6-codon sequence with mCherry and GFP reporters design  
BBa_K933002 device AHL leader with mCherry and GFP reporters design  

Continuing Research

Codon Optimization

Sequence Name Status
theonine 9-repeat codon_1 oligos annealed
theonine 9-repeat codon_2 oligos annealed
theonine 9-repeat codon_3 oligos annealed
theonine 9-repeat codon_alternating oligos annealed
alanine 9-repeat codon_1 oligos annealed
alanine 9-repeat codon_2 oligos annealed
alanine 9-repeat codon_3 oligos annealed
alanine 9-repeat codon_alternating oligos annealed
theonine 6-repeat codon_1 complete and sequenced
theonine 6-repeat codon_2 complete and sequenced yes
theonine 6-repeat codon_3 complete
theonine 6-repeat codon_alternating complete
alanine 6-repeat codon_1 oligos annealed
alanine 6-repeat codon_2 oligos annealed
alanine 6-repeat codon_3 oligos annealed
alanine 6-repeat codon_alternating oligos annealed
threonine 3-repeat codon_1 oligos annealed
threonine 3-repeat codon_2 oligos annealed
threonine 3-repeat codon_3 oligos annealed
threonine 3-repeat codon_alternating oligos annealed  

Bidirectional Promoters

Sequence Name Status Part Sequenced
BBa_K933004 complete yes
BBa_K933005 complete and added to Registry yes
BBa_K933006 complete and added to Registry yes
BBa_K933003 complete and added to Registry yes
testing promoter BBa_J23103 oligos annealed  
testing promoter BBa_J23114 oligos annealed  

Multiple Start Codons

Sequence Name Status
RBS1strong_RBS2weak small length oligos annealed
RBS1weak_RBS2strong small length oligos annealed
RBS1strong_RBS2strong small length oligos annealed
RBS1strong_RBS2weak med length oligos annealed
RBS1weak_RBS2strong med length complete and sequenced
RBS1strong_RBS2strong med length order oligos
RBS1strong_RBS2weak med length oligos annealed
RBS1weak_RBS2strong med length oligos annealed
RBS1strong_RBS2strong med length order oligos