Team:Penn State/Parts

From 2012.igem.org

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<a class="navitab" href="http://2012.igem.org/Team:Penn_State/Project">Projects</a><span class="hide"> | </span>
<a class="navitab" href="http://2012.igem.org/Team:Penn_State/Project">Projects</a><span class="hide"> | </span>
<a class="activenavitab" href="http://2012.igem.org/Team:Penn_State/Parts">Parts</a><span class="hide"> | </span>
<a class="activenavitab" href="http://2012.igem.org/Team:Penn_State/Parts">Parts</a><span class="hide"> | </span>
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<a class="navitab" href="http://2012.igem.org/Team:Penn_State/Modeling">Modeling</a><span class="hide"> | </span>
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<a class="navitab" href="http://2012.igem.org/Team:Penn_State/Modeling">Main Results</a><span class="hide"> | </span>
<a class="navitab" href="http://2012.igem.org/Team:Penn_State/Notebook">Notebook</a><span class="hide"> | </span>
<a class="navitab" href="http://2012.igem.org/Team:Penn_State/Notebook">Notebook</a><span class="hide"> | </span>
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<a class="navitab" href="http://2012.igem.org/Team:Penn_State/Safety">Other</a>
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<a class="navitab" href="http://2012.igem.org/Team:Penn_State/Other">Other</a>
</div>
</div>
<div id="desc">
<div id="desc">
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<h2>Parts Submitted to the Registry</h2>
+
<h3>Parts Submitted to the Registry</h3>
<p>Check out the parts we have submitted to the Registry of Standard Biological Parts! This year's parts include two of our main project constructs, as well as one successful test construct. Be on the lookout for many more to come!</p>
<p>Check out the parts we have submitted to the Registry of Standard Biological Parts! This year's parts include two of our main project constructs, as well as one successful test construct. Be on the lookout for many more to come!</p>
<p class="right"><a href="http://partsregistry.org/Main_Page">Registry of Biological Parts</a></p>
<p class="right"><a href="http://partsregistry.org/Main_Page">Registry of Biological Parts</a></p>
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<div id="main">
<div id="main">
 +
<h2>Submitted Parts</h2>
<table width="600" border="1">
<table width="600" border="1">
   <tr>
   <tr>
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     <td><a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K933000">BBa_K933000</a></td>
     <td><a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K933000">BBa_K933000</a></td>
     <td>device</td>
     <td>device</td>
-
     <td>multiple start codons coding for RFP and sfGFP respetively</td>
+
     <td>multiple start codons coding for RFP and sfGFP respectively</td>
-
     <td>&nbsp;</td>
+
     <td><a href="http://2012.igem.org/wiki/images/5/54/Msc3.jpg">design</a></td>
     <td>&nbsp;</td>
     <td>&nbsp;</td>
   </tr>
   </tr>
   <tr>
   <tr>
-
     <td><a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K933000">BBa_K933001</a></td>
+
     <td><a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K933001">BBa_K933001</a></td>
     <td>device</td>
     <td>device</td>
-
     <td>repeating theonine 6-codon sequence with mCherry and GFP reporters</td>
+
     <td>repeating threonine 6-codon sequence with mCherry and GFP reporters</td>
-
     <td>&nbsp;</td>
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     <td><a href="http://2012.igem.org/wiki/images/8/87/Codopt1.jpg">design</a></td>
     <td>&nbsp;</td>
     <td>&nbsp;</td>
   </tr>
   </tr>
   <tr>
   <tr>
-
     <td><a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K933000">BBa_K933002</a></td>
+
     <td><a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K933002">BBa_K933002</a></td>
     <td>device</td>
     <td>device</td>
     <td>AHL leader with mCherry and GFP reporters</td>
     <td>AHL leader with mCherry and GFP reporters</td>
 +
    <td><a href="http://2012.igem.org/wiki/images/2/27/Codopt2.jpg">design</a></td>
     <td>&nbsp;</td>
     <td>&nbsp;</td>
 +
  </tr>
 +
</table>
 +
</br>
 +
 +
<h2>Continuing Research</h2>
 +
<h3><a href="http://2012.igem.org/Team:Penn_State/Codon_Optimization">Codon Optimization</a></h3>
 +
<table width="600" border="1">
 +
  <tr>
 +
    <th scope="col">Sequence Name</th>
 +
    <th scope="col">Status</th>
 +
 +
   
 +
  <tr>
 +
    <td>theonine 9-repeat codon_1</td>
 +
    <td>oligos annealed</td>
 +
 +
  </tr>
 +
  <tr>
 +
    <td>theonine 9-repeat codon_2</td>
 +
    <td>oligos annealed</td>
 +
 +
 +
  </tr>
 +
  <tr>
 +
    <td>theonine 9-repeat codon_3</td>
 +
    <td>oligos annealed</td>
 +
 +
 +
  </tr>
 +
  <tr>
 +
    <td>theonine 9-repeat codon_alternating</td>
 +
    <td>oligos annealed</td>
 +
 +
 +
  </tr>
 +
  <tr>
 +
    <td>alanine 9-repeat codon_1</td>
 +
    <td>oligos annealed</td>
 +
 +
 +
  </tr>
 +
  <tr>
 +
    <td>alanine 9-repeat codon_2</td>
 +
    <td>oligos annealed</td>
 +
 +
  </tr>
 +
  <tr>
 +
    <td>alanine 9-repeat codon_3</td>
 +
    <td>oligos annealed</td>
 +
 +
  </tr>
 +
  <tr>
 +
    <td>alanine 9-repeat codon_alternating</td>
 +
    <td>oligos annealed</td>
 +
 +
 +
  </tr>
 +
  <tr>
 +
    <td>theonine 6-repeat codon_1</td>
 +
    <td>complete and sequenced</td>
 +
 +
 +
  </tr>
 +
  <tr>
 +
    <td>theonine 6-repeat codon_2</td>
 +
    <td>complete and sequenced</td>
 +
    <td>yes</td>
 +
  </tr>
 +
  <tr>
 +
    <td>theonine 6-repeat codon_3</td>
 +
    <td>complete</td>
 +
 +
 +
  </tr>
 +
  <tr>
 +
    <td>theonine 6-repeat codon_alternating</td>
 +
    <td>complete</td>
 +
 +
 +
  </tr>
 +
  <tr>
 +
    <td>alanine 6-repeat codon_1</td>
 +
    <td>oligos annealed</td>
 +
 +
 +
  </tr>
 +
  <tr>
 +
    <td>alanine 6-repeat codon_2</td>
 +
    <td>oligos annealed</td>
 +
 +
 +
  </tr>
 +
  <tr>
 +
    <td>alanine 6-repeat codon_3</td>
 +
    <td>oligos annealed</td>
 +
 +
 +
  </tr>
 +
  <tr>
 +
    <td>alanine 6-repeat codon_alternating</td>
 +
    <td>oligos annealed</td>
 +
 +
 +
  </tr>
 +
  <tr>
 +
    <td>threonine 3-repeat codon_1</td>
 +
    <td>oligos annealed</td>
 +
 +
 +
  </tr>
 +
  <tr>
 +
    <td>threonine 3-repeat codon_2</td>
 +
    <td>oligos annealed</td>
 +
 +
 +
  </tr>
 +
  <tr>
 +
    <td>threonine 3-repeat codon_3</td>
 +
    <td>oligos annealed</td>
 +
 +
 +
  </tr>
 +
  <tr>
 +
    <td>threonine 3-repeat codon_alternating</td>
 +
    <td>oligos annealed</td>
     <td>&nbsp;</td>
     <td>&nbsp;</td>
 +
 +
  </tr>
 +
 
 +
</table>
 +
<br/>
 +
<h3><a href="http://2012.igem.org/Team:Penn_State/Bidirectional_Promoters
 +
">Bidirectional Promoters</a></h3>
 +
 +
<table width="600" border="1">
 +
  <tr>
 +
    <th scope="col">Sequence Name</th>
 +
    <th scope="col">Status</th>
 +
    <th scope="col">Part Sequenced</th>
 +
  </tr>
 +
  <tr>
 +
    <td><a href="http://partsregistry.org/Part:BBa_K933004">BBa_K933004</a></td>
 +
    <td>complete</td>
 +
    <td>yes</td>
 +
  </tr>
 +
  <tr>
 +
    <td><a href="http://partsregistry.org/Part:BBa_K933005">BBa_K933005</a></td>
 +
    <td>complete and added to Registry</td>
 +
    <td>yes</td>
 +
  </tr>
 +
  <tr>
 +
    <td><a href="http://partsregistry.org/Part:BBa_K933006">BBa_K933006</a></td>
 +
    <td>complete and added to Registry</td>
 +
    <td>yes</td>
 +
  </tr>
 +
  <tr>
 +
    <td><a href="http://partsregistry.org/Part:BBa_K933003">BBa_K933003</a></td>
 +
    <td>complete and added to Registry</td>
 +
    <td>yes</td>
 +
  </tr>
 +
  <tr>
 +
    <td>testing promoter BBa_J23103</td>
 +
    <td>oligos annealed</td>
 +
    <td>&nbsp;</td>
 +
  </tr>
 +
  <tr>
 +
    <td>testing promoter BBa_J23114</td>
 +
    <td>oligos annealed</td>
 +
    <td>&nbsp;</td>
 +
  </tr>
 +
</table>
 +
 +
<br/>
 +
<h3><a href="http://2012.igem.org/Team:Penn_State/Multiple_Start_Codons">Multiple Start Codons</a></h3>
 +
 +
<table width="600" border="1">
 +
  <tr>
 +
    <th scope="col">Sequence Name</th>
 +
    <th scope="col">Status</th>
 +
 +
  </tr>
 +
  <tr>
 +
    <td>RBS1strong_RBS2weak small length</td>
 +
    <td>oligos annealed</td>
 +
 +
  </tr>
 +
  <tr>
 +
    <td>RBS1weak_RBS2strong small length</td>
 +
    <td>oligos annealed</td>
 +
 +
  </tr>
 +
  <tr>
 +
    <td>RBS1strong_RBS2strong small length</td>
 +
    <td>oligos annealed</td>
 +
 +
  </tr>
 +
  <tr>
 +
    <td>RBS1strong_RBS2weak med length</td>
 +
    <td>oligos annealed</td>
 +
 +
  </tr>
 +
  <tr>
 +
    <td>RBS1weak_RBS2strong med length</td>
 +
    <td>complete and sequenced</td>
 +
 +
  </tr>
 +
  <tr>
 +
    <td>RBS1strong_RBS2strong med length</td>
 +
    <td>order oligos</td>
 +
 +
  </tr>
 +
  <tr>
 +
    <td>RBS1strong_RBS2weak med length</td>
 +
    <td>oligos annealed</td>
 +
 +
  </tr>
 +
  <tr>
 +
    <td>RBS1weak_RBS2strong med length</td>
 +
    <td>oligos annealed</td>
 +
 +
  </tr>
 +
  <tr>
 +
    <td>RBS1strong_RBS2strong med length</td>
 +
    <td>order oligos</td>
 +
   </tr>
   </tr>
</table>
</table>
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</ul>
</ul>
-
<h3>Picture Gallery</h3>
+
-
<img class="photo" src="images/test.jpg" height="100" width="130" alt="" />
+
<h3>Projects</h3>
<h3>Projects</h3>
     <ul>
     <ul>

Latest revision as of 03:01, 27 October 2012

Index

Parts Submitted to the Registry

Check out the parts we have submitted to the Registry of Standard Biological Parts! This year's parts include two of our main project constructs, as well as one successful test construct. Be on the lookout for many more to come!

Registry of Biological Parts

Submitted Parts

Name Type Description Symbolic Model Length (bp)
BBa_K933000 device multiple start codons coding for RFP and sfGFP respectively design  
BBa_K933001 device repeating threonine 6-codon sequence with mCherry and GFP reporters design  
BBa_K933002 device AHL leader with mCherry and GFP reporters design  

Continuing Research

Codon Optimization

Sequence Name Status
theonine 9-repeat codon_1 oligos annealed
theonine 9-repeat codon_2 oligos annealed
theonine 9-repeat codon_3 oligos annealed
theonine 9-repeat codon_alternating oligos annealed
alanine 9-repeat codon_1 oligos annealed
alanine 9-repeat codon_2 oligos annealed
alanine 9-repeat codon_3 oligos annealed
alanine 9-repeat codon_alternating oligos annealed
theonine 6-repeat codon_1 complete and sequenced
theonine 6-repeat codon_2 complete and sequenced yes
theonine 6-repeat codon_3 complete
theonine 6-repeat codon_alternating complete
alanine 6-repeat codon_1 oligos annealed
alanine 6-repeat codon_2 oligos annealed
alanine 6-repeat codon_3 oligos annealed
alanine 6-repeat codon_alternating oligos annealed
threonine 3-repeat codon_1 oligos annealed
threonine 3-repeat codon_2 oligos annealed
threonine 3-repeat codon_3 oligos annealed
threonine 3-repeat codon_alternating oligos annealed  

Bidirectional Promoters

Sequence Name Status Part Sequenced
BBa_K933004 complete yes
BBa_K933005 complete and added to Registry yes
BBa_K933006 complete and added to Registry yes
BBa_K933003 complete and added to Registry yes
testing promoter BBa_J23103 oligos annealed  
testing promoter BBa_J23114 oligos annealed  

Multiple Start Codons

Sequence Name Status
RBS1strong_RBS2weak small length oligos annealed
RBS1weak_RBS2strong small length oligos annealed
RBS1strong_RBS2strong small length oligos annealed
RBS1strong_RBS2weak med length oligos annealed
RBS1weak_RBS2strong med length complete and sequenced
RBS1strong_RBS2strong med length order oligos
RBS1strong_RBS2weak med length oligos annealed
RBS1weak_RBS2strong med length oligos annealed
RBS1strong_RBS2strong med length order oligos