Team:Penn State/Notebook

From 2012.igem.org

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<title>Penn State iGEM 2012</title>
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.Navigation{
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<div id="Penn State Team Navigation" style="text-align: left; >
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<b> Navigaiton </b> <br>
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<a href="http://partsregistry.org/Part:BBa_R0063"; title="BBa_R0063";> BBa_R0063</a>,
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text-align:left; margin-left:2px; width:750px; position:absolute; left:190px; top:145px;
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<b> Navigation </b> <br/>
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<a href="https://2012.igem.org/Team:Penn_State"; title="Penn State iGEM 2012 Home">Home </a><br/>
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<a href="https://2012.igem.org/Team:Penn_State/Team"; title="Penn State iGEM 2012 Team">Team </a><br/>
 +
<a href="https://igem.org/Team.cgi?year=2012"; title="Penn State iGEM 2012 Official Team Profile">Official Team Profile </a><br/>
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<a href="https://2012.igem.org/Team:Penn_State/Projects"; title="Penn State iGEM 2012 Projects">Projects </a><br/>
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<a href="https://2012.igem.org/Team:Penn_State/Parts"; title="Penn State iGEM 2012 Parts Submitted to the Registry">Parts Submitted to the Registry</a><br/>
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<a href="https://2012.igem.org/Team:Penn_State/Modeling"; title="Penn State iGEM 2012 Modeling">Modeling </a><br/>
 +
<a href="https://2012.igem.org/Team:Penn_State/Notebook"; title="Penn State iGEM 2012 Notebook">Notebook </a><br/>
 +
<a href="https://2012.igem.org/Team:Penn_State/Safety"; title="Penn State iGEM 2012 Safety">Safety </a><br/>
 +
<a href="https://2012.igem.org/Team:Penn_State/Attributions"; title="Penn State iGEM 2012 Attributions">Attributions </a><br/>
</div>
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<div id="Notebook" style="text-align: left; padding: 5px; font-size: large; font-weight: bold;">
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<div class="NotebookHeader">
Penn State Notebook
Penn State Notebook
</div>
</div>
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<div id="body" style=""width: 700px; margin-left: 137px; padding: 5px; boarder: 0px;">
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<div class="Entries">
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    <p>
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<p>
-
    <b>Monday, May 22, 2012</b><br/>
+
<b>Monday, May 22, 2012</b><br/>
-
We prepared ampicillin and streptomycin plates in preparation for production of plasmids and competent cells.  
+
We prepared ampicillin and streptomycin plates in preparation for production of plasmids and competent cells.  
-
<!-- Sgt_Bisendi -->
+
<!-- Sgt_Bisendi -->
</p>
</p>
-
    <p>
+
<p>
-
    <b>Tuesday, May 23, 2012</b><br/>
+
<b>Tuesday, May 23, 2012</b><br/>
-
Inoculation of LB broth with DH10B E. coli strain to prepare master mix/culture of cells and in preparation for making chemically competent cells. These cells were incubated throughout the day and prepared for competency by the end of the day. Promoters were also planned out for the Multiple Promoters Project <!-- href --> and ordered. These promoters were designed to combine some of the fluorescent components of the plasmid.
+
Inoculation of LB broth with DH10B E. coli strain to prepare master mix/culture of cells in preparation for making chemically competent cells. These cells were incubated throughout the day and prepared for competency by the end of the day. Promotes were also planned out for the Multiple Promoters Project <!-- href --> and ordered. These promoters were designed to combine some of the components of the plasmid.
-
<!-- Sgt_Bisendi -->
+
<!-- Sgt_Bisendi -->
</p>
</p>
-
    <p>  
+
 
-
    <b>Wednesday, May 24, 2012</b><br/>
+
<p>
-
Biobricks were hydrated and transformed into Chemically Competent cells through heat shock (
+
<b>Wednesday, May 24, 2012</b><br/>
-
        <a href="http://partsregistry.org/Part:BBa_R0063"; title="BBa_R0063";> BBa_R0063</a>,  
+
Biobricks were hydrated and transformed into Chemically Competent cells through heat shock (
-
        <a href="http://partsregistry.org/Part:BBa_R1062"; title="BBa_R1062";> BBa_R1062</a>,  
+
<a href="http://partsregistry.org/Part:BBa_R0063"; title="BBa_R0063";> BBa_R0063</a>,  
-
        <a href="http://partsregistry.org/Part:BBa_R0078"; title="BBa_R0078";> BBa_R0078</a>,  
+
<a href="http://partsregistry.org/Part:BBa_R1062"; title="BBa_R1062";> BBa_R1062</a>,  
-
        <a href="http://partsregistry.org/Part:BBa_I1051"; title="BBa_I1051";> BBa_I1051</a>,  
+
<a href="http://partsregistry.org/Part:BBa_R0078"; title="BBa_R0078";> BBa_R0078</a>,  
-
        <a href="http://partsregistry.org/Part:BBa_R0079"; title="BBa_R0079";> BBa_R0079</a>,  
+
<a href="http://partsregistry.org/Part:BBa_I1051"; title="BBa_I1051";> BBa_I1051</a>,  
-
        <a href="http://partsregistry.org/Part:BBa_R0071"; title="BBa_R0071";> BBa_R0071</a>
+
<a href="http://partsregistry.org/Part:BBa_R0079"; title="BBa_R0079";> BBa_R0079</a>,  
-
        ). LOB broth was also prepared for the recovery from the transformation. These cells were then plated to produce isolated colonies.
+
<a href="http://partsregistry.org/Part:BBa_R0071"; title="BBa_R0071";> BBa_R0071</a>
-
<!-- Sgt_Bisendi -->
+
). LOB broth was also prepared for the recovery from the transformation. These cells were then plated to produce isolated colonies.
 +
<!-- Sgt_Bisendi -->
</p>
</p>
-
  <p>  
+
<p>
-
  <b>Thursday, May 25, 2012</b><br/>
+
<b>Thursday, May 25, 2012</b><br/>
-
Additional Biobricks were hydrated and transformed into Chemically Competent cells through heat shock (
+
Additional Biobricks were hydrated and transformed into Chemically Competent cells through heat shock (
-
        <a href="http://partsregistry.org/Part:BBa_I712074"; title="BBa_I712074"> BBa_I712074</a>,
+
<a href="http://partsregistry.org/Part:BBa_I712074"; title="BBa_I712074"> BBa_I712074</a>,
-
        <a href="http://partsregistry.org/Part:BBa_R0062"; title="BBa_R0062"> BBa_R0062</a>,
+
<a href="http://partsregistry.org/Part:BBa_R0062"; title="BBa_R0062"> BBa_R0062</a>,
-
        <a href="http://partsregistry.org/Part:BBa_I746104"; title="BBa_I746104"> BBa_I746104</a>,
+
<a href="http://partsregistry.org/Part:BBa_I746104"; title="BBa_I746104"> BBa_I746104</a>,
-
        <a href="http://partsregistry.org/Part:BBa_I719005"; title="BBa_I719005"> BBa_I719005</a>,
+
<a href="http://partsregistry.org/Part:BBa_I719005"; title="BBa_I719005"> BBa_I719005</a>,
-
        <a href="http://partsregistry.org/Part:BBa_J23119"; title="BBa_J23119"> BBa_J23119</a>,
+
<a href="http://partsregistry.org/Part:BBa_J23119"; title="BBa_J23119"> BBa_J23119</a>,
-
        <a href="http://partsregistry.org/Part:BBa_J23100"; title="BBa_J23100"> BBa_J23100</a>,
+
<a href="http://partsregistry.org/Part:BBa_J23100"; title="BBa_J23100"> BBa_J23100</a>,
-
        <a href="http://partsregistry.org/Part:BBa_J23101"; title="BBa_J23101"> BBa_J23101</a>,
+
<a href="http://partsregistry.org/Part:BBa_J23101"; title="BBa_J23101"> BBa_J23101</a>,
-
        <a href="http://partsregistry.org/Part:BBa_J23102"; title="BBa_J23102"> BBa_J23102</a>,
+
<a href="http://partsregistry.org/Part:BBa_J23102"; title="BBa_J23102"> BBa_J23102</a>,
-
        <a href="http://partsregistry.org/Part:BBa_J23103"; title="BBa_J23103"> BBa_J23103</a>,
+
<a href="http://partsregistry.org/Part:BBa_J23103"; title="BBa_J23103"> BBa_J23103</a>,
-
        <a href="http://partsregistry.org/Part:BBa_J23104"; title="BBa_J23104"> BBa_J23104</a>,
+
<a href="http://partsregistry.org/Part:BBa_J23104"; title="BBa_J23104"> BBa_J23104</a>,
-
        <a href="http://partsregistry.org/Part:BBa_J23105"; title="BBa_J23105"> BBa_J23105</a>,
+
<a href="http://partsregistry.org/Part:BBa_J23105"; title="BBa_J23105"> BBa_J23105</a>,
-
        <a href="http://partsregistry.org/Part:BBa_J23106"; title="BBa_J23106"> BBa_J23106</a>,
+
<a href="http://partsregistry.org/Part:BBa_J23106"; title="BBa_J23106"> BBa_J23106</a>,
-
        <a href="http://partsregistry.org/Part:BBa_J23107"; title="BBa_J23107"> BBa_J23107</a>,
+
<a href="http://partsregistry.org/Part:BBa_J23107"; title="BBa_J23107"> BBa_J23107</a>,
-
        <a href="http://partsregistry.org/Part:BBa_J23110"; title="BBa_J23110"> BBa_J23110</a>
+
<a href="http://partsregistry.org/Part:BBa_J23110"; title="BBa_J23110"> BBa_J23110</a>
-
        ). LOB broth was used to help the cells recover from their transformation. More agar plates were poured. The Transformed cells form yesterday yielded isolated colonies. A single colony from each plate was used to create a broth in preparation for mini-prep of the plasmids.  
+
). LOB broth was used to help the cells recover from their transformation. More agar plates were poured. The Transformed cells form yesterday yielded isolated colonies. A single colony from each plate was used to create a broth in preparation for mini-prep of the plasmids.  
-
        <!-- Sgt_Bisendi -->
+
      <!-- Sgt_Bisendi -->
-
        </p>
+
</p>
-
 
+
-
        <p>
+
-
        <b>Friday, May 25, 2012</b><br/>
+
-
Miniprep was done to extract the first six plasmids we transformed (
+
-
        <a href="http://partsregistry.org/Part:BBa_R0063"; title="BBa_R0063";> BBa_R0063</a>,
+
-
        <a href="http://partsregistry.org/Part:BBa_R1062"; title="BBa_R1062";> BBa_R1062</a>,
+
-
        <a href="http://partsregistry.org/Part:BBa_R0078"; title="BBa_R0078";> BBa_R0078</a>,
+
-
        <a href="http://partsregistry.org/Part:BBa_I1051"; title="BBa_I1051";> BBa_I1051</a>,
+
-
        <a href="http://partsregistry.org/Part:BBa_R0079"; title="BBa_R0079";> BBa_R0079</a>,
+
-
        <a href="http://partsregistry.org/Part:BBa_R0071"; title="BBa_R0071";> BBa_R0071</a>
+
-
). Colonies that grew from the transformation of our last 14 plasmids (
+
-
        <a href="http://partsregistry.org/Part:BBa_I712074"; title="BBa_I712074"> BBa_I712074</a>,
+
-
        <a href="http://partsregistry.org/Part:BBa_R0062"; title="BBa_R0062"> BBa_R0062</a>,
+
-
        <a href="http://partsregistry.org/Part:BBa_I746104"; title="BBa_I746104"> BBa_I746104</a>,
+
-
        <a href="http://partsregistry.org/Part:BBa_I719005"; title="BBa_I719005"> BBa_I719005</a>,
+
-
        <a href="http://partsregistry.org/Part:BBa_J23119"; title="BBa_J23119"> BBa_J23119</a>,
+
-
        <a href="http://partsregistry.org/Part:BBa_J23100"; title="BBa_J23100"> BBa_J23100</a>,
+
-
        <a href="http://partsregistry.org/Part:BBa_J23101"; title="BBa_J23101"> BBa_J23101</a>,
+
-
        <a href="http://partsregistry.org/Part:BBa_J23102"; title="BBa_J23102"> BBa_J23102</a>,
+
-
        <a href="http://partsregistry.org/Part:BBa_J23103"; title="BBa_J23103"> BBa_J23103</a>,
+
-
        <a href="http://partsregistry.org/Part:BBa_J23104"; title="BBa_J23104"> BBa_J23104</a>,
+
-
        <a href="http://partsregistry.org/Part:BBa_J23105"; title="BBa_J23105"> BBa_J23105</a>,
+
-
        <a href="http://partsregistry.org/Part:BBa_J23106"; title="BBa_J23106"> BBa_J23106</a>,
+
-
        <a href="http://partsregistry.org/Part:BBa_J23107"; title="BBa_J23107"> BBa_J23107</a>,
+
-
        <a href="http://partsregistry.org/Part:BBa_J23110"; title="BBa_J23110"> BBa_J23110</a>
+
-
) were cultured for later plasmid extraction.
+
-
        <!-- Sgt_Bisendi -->
+
-
        </p>
+
-
 
+
-
 
+
-
 
+
-
 
+
-
 
+
-
 
+
 +
<p>
 +
<b>Friday, May 25, 2012</b><br/>
 +
Miniprep was done to extract the first six plasmids we transformed (
 +
<a href="http://partsregistry.org/Part:BBa_R0063"; title="BBa_R0063";> BBa_R0063</a>,
 +
<a href="http://partsregistry.org/Part:BBa_R1062"; title="BBa_R1062";> BBa_R1062</a>,
 +
<a href="http://partsregistry.org/Part:BBa_R0078"; title="BBa_R0078";> BBa_R0078</a>,
 +
<a href="http://partsregistry.org/Part:BBa_I1051"; title="BBa_I1051";> BBa_I1051</a>,
 +
<a href="http://partsregistry.org/Part:BBa_R0079"; title="BBa_R0079";> BBa_R0079</a>,
 +
<a href="http://partsregistry.org/Part:BBa_R0071"; title="BBa_R0071";> BBa_R0071</a>
 +
). Colonies that grew from the transformation of our last 14 plasmids (
 +
<a href="http://partsregistry.org/Part:BBa_I712074"; title="BBa_I712074"> BBa_I712074</a>,
 +
<a href="http://partsregistry.org/Part:BBa_R0062"; title="BBa_R0062"> BBa_R0062</a>,
 +
<a href="http://partsregistry.org/Part:BBa_I746104"; title="BBa_I746104"> BBa_I746104</a>,
 +
<a href="http://partsregistry.org/Part:BBa_I719005"; title="BBa_I719005"> BBa_I719005</a>,
 +
<a href="http://partsregistry.org/Part:BBa_J23119"; title="BBa_J23119"> BBa_J23119</a>,
 +
<a href="http://partsregistry.org/Part:BBa_J23100"; title="BBa_J23100"> BBa_J23100</a>,
 +
<a href="http://partsregistry.org/Part:BBa_J23101"; title="BBa_J23101"> BBa_J23101</a>,
 +
<a href="http://partsregistry.org/Part:BBa_J23102"; title="BBa_J23102"> BBa_J23102</a>,
 +
<a href="http://partsregistry.org/Part:BBa_J23103"; title="BBa_J23103"> BBa_J23103</a>,
 +
<a href="http://partsregistry.org/Part:BBa_J23104"; title="BBa_J23104"> BBa_J23104</a>,
 +
<a href="http://partsregistry.org/Part:BBa_J23105"; title="BBa_J23105"> BBa_J23105</a>,
 +
<a href="http://partsregistry.org/Part:BBa_J23106"; title="BBa_J23106"> BBa_J23106</a>,
 +
<a href="http://partsregistry.org/Part:BBa_J23107"; title="BBa_J23107"> BBa_J23107</a>,
 +
<a href="http://partsregistry.org/Part:BBa_J23110"; title="BBa_J23110"> BBa_J23110</a>
 +
) were cultured for later plasmid extraction.
 +
<!-- Sgt_Bisendi -->
 +
</p>
 +
<p>
 +
<b>Saturday, May 26, 2012</b><br/>
 +
Hannah and Kevin extracted the plasmids from the 14 colonies that were cultured yesterday. Two of the BioBricks that needed to be retransformed (
 +
<a href="http://partsregistry.org/Part:BBa_J23102"; title="BBa_J23102"> BBa_J23102</a>,
 +
<a href="http://partsregistry.org/Part:BBa_J23105"; title="BBa_J23105"> BBa_J23105</a>)
 +
had isolated colonies on their plates and were cultured by Kevin for plasmid extraction the next day.
 +
<!-- Sgt_Bisendi -->
 +
</p>
 +
<p>
 +
<b>Sunday, May 27, 2012</b><br/>
 +
Hannah and Kevin extracted the two cultures that Kevin prepared yesterday. These plasmids were put on ice for concentration analysis later with the other extracted plasmids.
 +
<!--Sgt_Bisendi -->
 +
</p>
<!-- Instructions on posting to the Notebook
<!-- Instructions on posting to the Notebook
-
         1. Make sure there is a <p> between the new entry and the previous one. This creates a new paragraph for the new entry.
+
         1. Enter a space between the last entry and the new one you are entering.
-
         2. Enter a <div>. This creates a new division/section for the new entry.
+
         2. Open your entry with a <p> to make a new paragraph for you text.
-
         3. Enter in <div><b> The date </b></div>. This creates the date in bold and puts it in its own line.
+
         3. Enter a <b> The Date </b> tag to make the text bold for the date.
-
         4. Enter your text on the next line. This is whatever you want to post for the notebook.
+
        4. Enter a <br/> after the date to crate a carriage return to the next line.
-
         5. After the notebook entry, put a < !-- user name -- >, without the spaces between the carrots and !/-. This posts your name so we know who authored the entry. This will not show up on the actual website. This is just for convenience.  
+
         5. Enter your text for the notebook.
-
         6. Finally, put a </div> to close the entry division/section. You can also put a <p> if you want to make space for the next post.
+
         6. On the next new line enter </p> to end your paragraph and entry.
 +
        7. Hit the "Save Page" button at the bottom of the page to update the page and save it for everyone to see.
 +
         8. Congratulations! You have just written code in HTML. Go have a cookie!
-->
-->
</div>
</div>
 +
</html>
</html>
-
 
-
 
-
{| style="color:#1b2c8a;background-color:#58ACFA;" cellpadding="2px" cellspacing="5px" border="0px" bordercolor="#fff" width="75%" align="center"
 
-
!align="center"|[[Team:Penn_State|Home]]
 
-
!align="center"|[[Team:Penn_State/Team|Team]]
 
-
!align="center"|[https://igem.org/Team.cgi?year=2012&team_name=Penn_State Official Team Profile]
 
-
!align="center"|[[Team:Penn_State/Project|Project]]
 
-
!align="center"|[[Team:Penn_State/Parts|Parts Submitted to the Registry]]
 
-
!align="center"|[[Team:Penn_State/Modeling|Modeling]]
 
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!align="center"|[[Team:Penn_State/Notebook|Notebook]]
 
-
!align="center"|[[Team:Penn_State/Safety|Safety]]
 
-
!align="center"|[[Team:Penn_State/Attributions|Attributions]]
 
-
|}
 

Revision as of 15:46, 27 May 2012

Penn State iGEM 2012

Navigation
Home
Team
Official Team Profile
Projects
Parts Submitted to the Registry
Modeling
Notebook
Safety
Attributions
Penn State Notebook

Monday, May 22, 2012
We prepared ampicillin and streptomycin plates in preparation for production of plasmids and competent cells.

Tuesday, May 23, 2012
Inoculation of LB broth with DH10B E. coli strain to prepare master mix/culture of cells in preparation for making chemically competent cells. These cells were incubated throughout the day and prepared for competency by the end of the day. Promotes were also planned out for the Multiple Promoters Project and ordered. These promoters were designed to combine some of the components of the plasmid.

Wednesday, May 24, 2012
Biobricks were hydrated and transformed into Chemically Competent cells through heat shock ( BBa_R0063, BBa_R1062, BBa_R0078, BBa_I1051, BBa_R0079, BBa_R0071 ). LOB broth was also prepared for the recovery from the transformation. These cells were then plated to produce isolated colonies.

Thursday, May 25, 2012
Additional Biobricks were hydrated and transformed into Chemically Competent cells through heat shock ( BBa_I712074, BBa_R0062, BBa_I746104, BBa_I719005, BBa_J23119, BBa_J23100, BBa_J23101, BBa_J23102, BBa_J23103, BBa_J23104, BBa_J23105, BBa_J23106, BBa_J23107, BBa_J23110 ). LOB broth was used to help the cells recover from their transformation. More agar plates were poured. The Transformed cells form yesterday yielded isolated colonies. A single colony from each plate was used to create a broth in preparation for mini-prep of the plasmids.

Friday, May 25, 2012
Miniprep was done to extract the first six plasmids we transformed ( BBa_R0063, BBa_R1062, BBa_R0078, BBa_I1051, BBa_R0079, BBa_R0071 ). Colonies that grew from the transformation of our last 14 plasmids ( BBa_I712074, BBa_R0062, BBa_I746104, BBa_I719005, BBa_J23119, BBa_J23100, BBa_J23101, BBa_J23102, BBa_J23103, BBa_J23104, BBa_J23105, BBa_J23106, BBa_J23107, BBa_J23110 ) were cultured for later plasmid extraction.

Saturday, May 26, 2012
Hannah and Kevin extracted the plasmids from the 14 colonies that were cultured yesterday. Two of the BioBricks that needed to be retransformed ( BBa_J23102, BBa_J23105) had isolated colonies on their plates and were cultured by Kevin for plasmid extraction the next day.

Sunday, May 27, 2012
Hannah and Kevin extracted the two cultures that Kevin prepared yesterday. These plasmids were put on ice for concentration analysis later with the other extracted plasmids.

Retrieved from "http://2012.igem.org/Team:Penn_State/Notebook"