Team:Penn State/Modeling

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Revision as of 03:39, 26 October 2012

Bidirectional Promoters Overview

Bidirectional Promoters

Scientists are frequently confounded by wayward promoters; that is, promoters which do not produce the expected proteins. Some bidirectional promoters are known to exist, but which way they promote and the degree of expression has not been quantified. This project will test the directionality of several BioBrick promoters to answer these questions.

BiDirectional Promoter Project Data

Here we can see some of the data collected for the Bidirectional Promoter Project. The data shows the fluorescence of GFP and RFP from different promoters collected by the TCAN.

GFP Data for all of the samples tested and the average of those samples for a given construct. The promoters tested so far are BBa_J23102, and three custom palindromic promoters.



RFP Data for all of the samples tested and the average of those samples is shown below.


Multiple Start Codon Project Data

Here is some of the preliminary data for the MSC Project. The graph depicts the fluorescence measured by expression from each individual start codon.