Team:Penn State/Codon Optimization Results

From 2012.igem.org

(Difference between revisions)
(Created page with "<html> <head> <title>Bidirectional Promoters Overview</title> <style type="text/css"> body { background:#e0e0e0; color:#303030; font:76% tahoma, verdana, sans-serif; margin...")
Line 58: Line 58:
text-decoration:none;
text-decoration:none;
}
}
 +
a:visited {color:#666;}
a img {
a img {
Line 143: Line 144:
margin: 5px 0 15px;
margin: 5px 0 15px;
padding: 0 0 5px 0;
padding: 0 0 5px 0;
-
background-image: url(http://2012.igem.org/wiki/images/5/55/IndexNav1.jpg);
+
background-image: url(http://2012.igem.org/wiki/images/a/aa/Nav3.jpg);
background-repeat: no-repeat;
background-repeat: no-repeat;
background-position: left bottom;
background-position: left bottom;
Line 294: Line 295:
}
}
-
#logoiGEM {
 
-
height: 114px;
 
-
width: 280px;
 
-
float: right;
 
-
background-image: url(http://2012.igem.org/wiki/images/4/4b/Sweatshirt_Logo.jpg);
 
-
padding-bottom: 15px;
 
-
background-repeat: no-repeat;
 
-
}
 
-
 
-
#logoPSU {
 
-
height: 54px;
 
-
width: 116px;
 
-
background-image: url(http://2012.igem.org/wiki/images/f/f9/PsuLogo.jpeg);
 
-
}
 
Line 389: Line 376:
}
}
 +
#logoiGEM {
 +
height: 56px;
 +
width: 140px;
 +
float: right;
 +
background-image: url(http://2012.igem.org/wiki/images/e/e9/Sweatshirt_Logofin.jpg);
 +
margin-bottom: 15px;
 +
background-repeat: no-repeat;
 +
}
 +
#psuLogo {
 +
        height: 65px;
 +
        width: 116;
 +
        float: right;
 +
        padding-top: 11px;
 +
}
</style>
</style>
</head>
</head>
-
<body>
 
 +
<body>
<div id="container">
<div id="container">
   <div id="logoiGEM"></div>
   <div id="logoiGEM"></div>
-
   <div id="logoPSU"></div>
+
   <div id="psuLogo"><img src="http://2012.igem.org/wiki/images/f/f9/PsuLogo.jpeg"></div>
   <div id="navitabs">
   <div id="navitabs">
     <h2 class="hide">Sample navigation menu:</h2>
     <h2 class="hide">Sample navigation menu:</h2>
Line 415: Line 416:
   <div id="main">
   <div id="main">
     <h2>Codon Optimization</h2>
     <h2>Codon Optimization</h2>
-
 
+
<iframe width="420" height="315" src="http://www.youtube.com/embed/uspXR2UzP0U" frameborder="0" allowfullscreen></iframe>
     <div id="maintabs">
     <div id="maintabs">
     <h2 class="hide">Sample navigation menu:</h2>
     <h2 class="hide">Sample navigation menu:</h2>

Revision as of 23:54, 3 October 2012

Bidirectional Promoters Overview

Codon Optimization

The genetic code is a degenerate one; there are more 3-part combinations of nucleotides than there are amino acids. The topic of codon optimization-that is, the cell's preference for one codon sequence over another in translation-has been heavily researched in an effort to determine the optimal genetic sequences for an organism. This project looks at the effects of repeated amino acid sequences of varying lengths and codons and their effect on the cell.

Codon Optimization

Sample navigation menu:

Overview | Design | Results

Data Collection

A repeated sequence of a single theonine codon was successfully ligated into the initial construct. The resulting fluorescence was measured using a TECAN machine. The results are displayed graphically below.

Additional Testing

Several more repeated sequences of identical and non-identical single amino acid codons are intended for testing in this construct. To view the completed and prospective sequences, please visit our Parts page