Team:Penn State/Bidirectional Promoters Results

From 2012.igem.org

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   <div id="desc">
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     <h3>Team Projects</h3>
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     <h3>Bidirectional Promoters</h3>
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     <p>Scientists are frequently confounded by wayward promoters; that is, promoters which do not produce
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     <p>Utilizing synthetic biology cloning techniques, the Penn State iGEM team has designed three genetic constructs that test the most basic assumptions of molecular biology.
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the expected proteins. Some bidirectional promoters are known to exist, but which way they promote
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While each of the individual projects looks at a different aspect of the mantra, they all come together under the umbrella of Questioning the Central Dogma of Molecular Biology.</p>
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and the degree of expression has not been quantified. This project will test the directionality of  
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    <p class="right"><a href="http://2012.igem.org/Team:Penn_State/Parts">Parts</a></div>
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several BioBrick promoters to answer these questions. </p>
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   <div id="main">
   <div id="main">
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    <h2>Bidirectional Promoters</h2>
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<h3>Measured Fluorescence</h3>
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    <div id="boxLeft"><a href="http://2012.igem.org/Team:Penn_State/Multiple_Start_Codons"><h3>Multiple Start Codons</h3></a>
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</div>
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      <p class="block">mRNA strands should have only one recognized start codon. What happens when multiple start codons are introduced in translation?</p>
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<p class="readmore"><a href="http://2012.igem.org/Team:Penn_State/Multiple_Start_Codons">read more...</a></p>                               
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    <div id="boxCenter"><a href="http://2012.igem.org/Team:Penn_State/Bidirectional_Promoters"><h3>Bidirectional Promoters</h3></a> 
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      <p class="block">Promoters usually initiate translation in one direction. Which promoters promote both foward and reverse on the mRNA strand?
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<p class="readmore"><a href="http://2012.igem.org/Team:Penn_State/Bidirectional_Promoters">read more...</a></p>
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    <div id="boxRight"><a href="http://2012.igem.org/Team:Penn_State/Codon_Optimization"><h3>Codon Optimization</h3></a>
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      <p class="block">Each organism has a preferred sequence for each amino acid. What happens when the cell is overloaded by one amino acid codon?<p class="readmore">
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<a href="http://2012.igem.org/Team:Penn_State/Codon_Optimization">read more...</a></p>
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     <h3>Check these Out!</h3>
     <h3>Check these Out!</h3>
     <ul class="sidelink">
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       <li><a href="http://2012.igem.org/Team:Penn_State/Judgin_Criteria">Judging Criteria</a></li>
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       <li><a href="http://2012.igem.org/Team:Penn_State/Judging_Criteria">Judging Criteria</a></li>
       <li><a href="http://2012.igem.org/Team:Penn_State/Parts">Parts</a></li>
       <li><a href="http://2012.igem.org/Team:Penn_State/Parts">Parts</a></li>
       <li><a href="http://2012.igem.org/Team:Penn_State/Human_Practices">Human Practices</a></li>
       <li><a href="http://2012.igem.org/Team:Penn_State/Human_Practices">Human Practices</a></li>
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    <h3>Picture Gallery</h3>
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<h3>Projects</h3>
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       <li><a href="http://2012.igem.org/Team:Penn_State/Multiple_Start_Codons">Multiple Start Codons</a></li>
       <li><a href="http://2012.igem.org/Team:Penn_State/Multiple_Start_Codons">Multiple Start Codons</a></li>
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     <p><strong>&copy; Hannah Jepsen-Burger<a href="#"></a></strong> | Follow us on Twitter<a href="#"></a></p>
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     <p><strong>&copy; Hannah Jepsen-Burger</strong> | Follow us on Twitter<a href="#"></a></p>
   </div>
   </div>
</div>
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Revision as of 03:30, 26 October 2012

Bidirectional Promoters Overview

Bidirectional Promoters

Scientists are frequently confounded by wayward promoters; that is, promoters which do not produce the expected proteins. Some bidirectional promoters are known to exist, but which way they promote and the degree of expression has not been quantified. This project will test the directionality of several BioBrick promoters to answer these questions.

Bidirectional Promoters

Sample navigation menu:

Overview | Design | Results

Measured Fluorescence