Team:Penn State/Bidirectional Promoters Results

From 2012.igem.org

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<a class="navitab" href="http://2012.igem.org/Team:Penn_State/Safety">Other</a> </div>
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  </div>
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   <div id="desc">
   <div id="desc">
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     <h2>Bidirectional Promoters</h2>
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     <h2>Team Projects</h2>
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     <p>Scientists are frequently confounded by wayward promoters; that is, promoters which do not produce
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the expected proteins. Some bidirectional promoters are known to exist, but which way they promote
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     <p>Utilizing synthetic biology cloning techniques, the Penn State iGEM team has designed three genetic constructs that test the most basic assumptions of molecular biology.
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and the degree of expression has not been quantified. This project will test the directionality of  
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While each of the individual projects looks at a different aspect of the mantra, they all come together under the umbrella of Questioning the Central Dogma of Molecular Biology.</p>
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several BioBrick promoters to answer these questions. </p>
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    <p class="right"><a href="http://2012.igem.org/Team:Penn_State/Parts">Parts</a></div>
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  </div>
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   <div id="main">
   <div id="main">
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     <h2>Bidirectional Promoters</h2>
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<iframe width="560" height="315" src="http://www.youtube.com/embed/s8eE07qU9yQ" frameborder="0" allowfullscreen></iframe>
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     <div id="boxLeft"><a href="http://2012.igem.org/Team:Penn_State/Multiple_Start_Codons"><h3>Multiple Start Codons</h3></a>
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      <p>    
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    <a class="maintab" href="http://2012.igem.org/Team:Penn_State/Bidirectional_Promoters">Overview</a>
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      <p>    
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    <span class="hide"> | </span> <a class="maintab" href="http://2012.igem.org/Team:Penn_State/Bidirectional_Promoters_Design">Design</a>
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      <p>    
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    <span class="hide"> | </span> <a class="activemaintab" href="http://2012.igem.org/Team:Penn_State/Bidirectional_Promoters_Results">Results</a>
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      <p class="block">mRNA strands should have only one recognized start codon. What happens when multiple start codons are introduced in translation?</p>
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    </div>
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<p class="readmore"><a href="http://2012.igem.org/Team:Penn_State/Multiple_Start_Codons">read more...</a></p>                                
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      </div>
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  <div id="tabfiller">
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<h3>Data Collection</h3>
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    <p>Several BioBrick promoters have been tested in the bidirectional promoter construct. All successful ligations were cultured and the fluorescence of each individual promoter was tested in a TECAN machine.</P>
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<p>The following promoters have been successfully tested for quantitative directionality using the TECAN. For a <a href="http://2012.igem.org/Team:Penn_State/Parts">complete list</a> of promoters we intend to test, please visit our <a href="http://2012.igem.org/Team:Penn_State/Parts">Parts</a> webpage.</p>
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    <div id="boxCenter"><a href="http://2012.igem.org/Team:Penn_State/Bidirectional_Promoters"><h3>Bidirectional Promoters</h3></a>
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<p>Data collection and results are demonstrated graphically below for J23102, and three custom palindrome promoters. You can also find the graphs on the <a href="http://2012.igem.org/Team:Penn_State/Modeling">Modeling Page</a>.(Click on the graphs to see a larger version.)</p>
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      <div id="center"><img src="http://2012.igem.org/wiki/images/e/e1/Bidir.jpg"></div>    
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<p>
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      <p>    
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GFP Expression of all samples: <br/>
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      <p>    
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<a href="http://2012.igem.org/wiki/images/3/3c/PennState_BiDir_GFP_All_Graph.jpg">
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      <p class="block">Promoters usually initiate translation in one direction. Which promoters promote both foward and reverse on the mRNA strand?
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<img src="http://2012.igem.org/wiki/images/3/3c/PennState_BiDir_GFP_All_Graph.jpg"/ width="500px" height="240px">
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<p class="readmore"><a href="http://2012.igem.org/Team:Penn_State/Bidirectional_Promoters">read more...</a></p>
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</a>
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      </div>
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</p>
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<p>
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Average GFP Expression of all samples: <br/>
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<a href="http://2012.igem.org/wiki/images/a/a9/PennState_BiDir_GFP_Combined_Graph.jpg">
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    <div id="boxRight"><a href="http://2012.igem.org/Team:Penn_State/Codon_Optimization"><h3>Codon Optimization</h3></a>
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<img src="http://2012.igem.org/wiki/images/a/a9/PennState_BiDir_GFP_Combined_Graph.jpg" width="300px" height="180px">
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    <div id="right"><img src="http://2012.igem.org/wiki/images/7/7a/Codopt.jpg"></div></p>  
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</a>
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      <p>    
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</p>
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      <p>    
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<p>
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      <p>     
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RFP Expression of all samples: <br/>
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      <p class="block">Each organism has a preferred sequence for each amino acid. What happens when the cell is overloaded by one amino acid codon?<p class="readmore">
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<a href="http://2012.igem.org/wiki/images/b/b6/PennState_BiDir_RFP_All_Graph.jpg">
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<a href="http://2012.igem.org/Team:Penn_State/Codon_Optimization">read more...</a></p>
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<img src="http://2012.igem.org/wiki/images/b/b6/PennState_BiDir_RFP_All_Graph.jpg" width="500px" height="240px">
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      </div>
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</a>
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</p>
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<p>
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Average RFP Expression of all samples: <br/>
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<a href="http://2012.igem.org/wiki/images/9/9f/PennState_BiDir_RFP_Combined_Graph.jpg">
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<img src="http://2012.igem.org/wiki/images/9/9f/PennState_BiDir_RFP_Combined_Graph.jpg" width="300px" height="180px">
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</a>
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</p>
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</div>
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   </div>
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   <div id="sidebar">
   <div id="sidebar">
     <h3>Check these Out!</h3>
     <h3>Check these Out!</h3>
     <ul class="sidelink">
     <ul class="sidelink">
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       <li><a href="http://2012.igem.org/Team:Penn_State/Judging_Criteria">Judging Criteria</a></li>
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       <li><a href="http://2012.igem.org/Team:Penn_State/Judgin_Criteria">Judging Criteria</a></li>
       <li><a href="http://2012.igem.org/Team:Penn_State/Parts">Parts</a></li>
       <li><a href="http://2012.igem.org/Team:Penn_State/Parts">Parts</a></li>
       <li><a href="http://2012.igem.org/Team:Penn_State/Human_Practices">Human Practices</a></li>
       <li><a href="http://2012.igem.org/Team:Penn_State/Human_Practices">Human Practices</a></li>
     </ul>
     </ul>
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    <h3>Picture Gallery</h3>
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    <img class="photo" src="../images/test.jpg" height="100" width="130" alt="" />
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  <h3>Photo Gallery</h3>
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    <h3>Projects</h3>
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<h3>Projects</h3>
     <ul>
     <ul>
       <li><a href="http://2012.igem.org/Team:Penn_State/Multiple_Start_Codons">Multiple Start Codons</a></li>
       <li><a href="http://2012.igem.org/Team:Penn_State/Multiple_Start_Codons">Multiple Start Codons</a></li>
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   <div id="footer">
   <div id="footer">
     <p><strong>&copy; Hannah Jepsen-Burger<a href="#"></a></strong> | Follow us on Twitter<a href="#"></a></p>
     <p><strong>&copy; Hannah Jepsen-Burger<a href="#"></a></strong> | Follow us on Twitter<a href="#"></a></p>
   </div>
   </div>
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Revision as of 05:22, 17 October 2012

Projects

Team Projects

Utilizing synthetic biology cloning techniques, the Penn State iGEM team has designed three genetic constructs that test the most basic assumptions of molecular biology. While each of the individual projects looks at a different aspect of the mantra, they all come together under the umbrella of Questioning the Central Dogma of Molecular Biology.

Parts

Multiple Start Codons

mRNA strands should have only one recognized start codon. What happens when multiple start codons are introduced in translation?

read more...

Bidirectional Promoters

Promoters usually initiate translation in one direction. Which promoters promote both foward and reverse on the mRNA strand?

read more...

Codon Optimization

Each organism has a preferred sequence for each amino acid. What happens when the cell is overloaded by one amino acid codon?

read more...