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Team:Penn/Notebook/DrugDelivery
From 2012.igem.org
(Difference between revisions)
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| - | '''06/07/2012''' | + | == '''06/07/2012''' == |
| - | + | '''Wet Lab''' | |
| - | + | ||
| + | Today we transformed both versions of cph8 from 2012 Distribution. | ||
| + | |||
| + | '''Dry Lab''' | ||
| + | |||
| + | We also placed order for the following new BioBricks: | ||
* BBa_K207001 - PhyB-DBD Fusion | * BBa_K207001 - PhyB-DBD Fusion | ||
* BBa_K422012 - Pif3 (Aar1 C part) | * BBa_K422012 - Pif3 (Aar1 C part) | ||
| Line 10: | Line 15: | ||
* BBa_K592000 - Cph8 | * BBa_K592000 - Cph8 | ||
* BBa_K365000 - Pif3 | * BBa_K365000 - Pif3 | ||
| + | In addition, we brainstormed and developed a new idea to use the PYP/GCN system to activate a gene. | ||
| + | |||
| + | == '''06/08/2012''' == | ||
| + | '''Wet Lab''' | ||
| + | |||
| + | No colonies appeared in the transformation of both cph8 a/b. We suspect that the failure of the transformatiosns were due to bad sequences. | ||
| + | |||
| + | '''06/11/2012''' | ||
| + | * Performed PCR on mCherry NAS 151 | ||
| + | * Did | ||
Revision as of 16:48, 26 June 2012
06/07/2012
Wet Lab
Today we transformed both versions of cph8 from 2012 Distribution.
Dry Lab
We also placed order for the following new BioBricks:
- BBa_K207001 - PhyB-DBD Fusion
- BBa_K422012 - Pif3 (Aar1 C part)
- BBa_K422013 - PhyB (Aar1 C part)
- BBa_K592006 - pFixK2
- BBa_K592004 - YF1
- BBa_K592005 - FixJ
- BBa_K592000 - Cph8
- BBa_K365000 - Pif3
In addition, we brainstormed and developed a new idea to use the PYP/GCN system to activate a gene.
06/08/2012
Wet Lab
No colonies appeared in the transformation of both cph8 a/b. We suspect that the failure of the transformatiosns were due to bad sequences.
06/11/2012
- Performed PCR on mCherry NAS 151
- Did
