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<b><div class="name" align="center">Objectives</div></b>
<b><div class="name" align="center">Objectives</div></b><br>
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<p style="color:black;text-indent:30px;">In order to develop a module for light activated cell lysis, we had to implement two elements:
<p style="color:black;text-indent:30px;">In order to develop a module for light activated cell lysis, we had to implement two elements:

Latest revision as of 03:32, 27 October 2012

Penn 2012 iGEM Wiki

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Light-Activated Cell Lysis


In order to develop a module for light activated cell lysis, we had to implement two elements:

  1. Construct a light-activation system that can express a downstream gene of interest.
  2. Express a cytolytic protein that can be expressed as our therapeutic drug to lyse cancer cells.
Objective 1: Light-Activated Sensor

Selection of YF1/FixJ Blue Light Sensor

After reading many papers to select an appropriate light-sensing system to use, we selected the YF1/FixJ blue light system. We had also considered the red light sensor Cph8 but ultimately decided on YF1/FixJ because of its high on/off ratio of gene expression and also because of its availability to us (we were fortunate enough to come across the YF1/FixJ system in the form of the pDawn plasmid from the Moglich lab in Germany).

YF1/FixJ System (pDawn)

As shown below in Figure 1, the YF1/FixJ system works through a "repress the repressor" concept. Upon 480 nm blue light illumination, YF1 (a fusion of a LOV protein domain and a histidine kinase) phosphorylates a FixJ response regulator that activates the pFixK2 promoter. The activation of pFixK2, promotes expression of the cI repressor that, in turn, represses the lambda promoter pR. The net result is activation of the gene in the downstream MCS.

Figure 1
Objective 2: Expression of a Cytolytic Protein

Cytolysin A (ClyA)

ClyA is a protein native to E. coli, Shigella flexneri, and Salmonella typhi that is capable of forming 13-mer pore complexes in a redox-independent manner. Expression of clyA in the absence of other hemolytic toxins is sufficient to induce hemolysis experimentally, and is therefore considered to be a potent cytolytic agent. Unlike a similar protein, HlyA, ClyA is not synthesized as a protoxin, which requires further posttranslational modifications to become active. ClyA is functional immediately following translation of mRNA to protein. ClyA is a 34kDa protein that is composed primarily of α-helical bundles that form a rod-shaped molecule. The membrane insertion domain is known as a β tongue (shown in yellow in Figure 2) and is critical for hemolytic activity. If the β tongue is mutated, the hemolytic activity of clyA is abrogated.

Figure 2
Figure 2: ClyA forms a 13-mer pore complex that consists of hydrophobic beta tongues (yellow) on the head domains of individual monomer units that play an important role in influencing its cytolytic functions.

Mechanism of Action

We selected ClyA as our cytolytic protein because of its unique mechanism of action that makes it especially potent. ClyA is secreted from bacteria in outer membrane vesicles (OMV's), within which it forms pore assemblies. As shown below in Figure 3, these pore assemblies allow ClyA to latch on to the cell wall of other cells and through the use of its encapsulating pore assembly lyse the cell wall.

Figure 3
Figure 3: Shown above in the first image are pore assemblies containing 13-mers of ClyA interacting with the surface of the target membrane. The second image below shows the ClyA assembly lysing the cell membrane through pore formation. (Wallace et. al 2000)