Team:Penn/LightActivatedOverview

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<p style="color:black;text-indent:30px;">In order to develop a module for light activated cell lysis, we had to implement two elements:
<p style="color:black;text-indent:30px;">In order to develop a module for light activated cell lysis, we had to implement two elements:
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<ul style="font-size:20px"><li><b>1) A light-activation system that can express a gene of interest</li>
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<ul style="font-size:20px"><li><b>1) A light-activation system that can express a gene of interest</b></li>
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<li>2) A cytotoxic protein that can be expressed as our therapeutic drug to lyse cancer sells</li>
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<li><b>2) A cytotoxic protein that can be expressed as our therapeutic drug to lyse cancer sells</b></li>
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Revision as of 23:02, 26 October 2012

Penn 2012 iGEM Wiki

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Light-Activated Cell Lysis

Objectives

In order to develop a module for light activated cell lysis, we had to implement two elements:

  • 1) A light-activation system that can express a gene of interest
  • 2) A cytotoxic protein that can be expressed as our therapeutic drug to lyse cancer sells
YF1/FixJ Characterization

After reading many papers to select an appropriate light-sensing system to use, we selected the YF1/FixJ blue light system. We had also considered the red light sensor Cph8 but ultimately decided on YF1/FixJ because of its high on/off ratio of gene expression and also because of its availability to us (we were fortunate enough to come across the YF1/FixJ system in the form of the pDawn plasmid from the Moglich lab in Germany).