http://2012.igem.org/wiki/index.php?title=Team:Paris_Bettencourt/Suicide&feed=atom&action=historyTeam:Paris Bettencourt/Suicide - Revision history2024-03-29T05:46:11ZRevision history for this page on the wikiMediaWiki 1.16.0http://2012.igem.org/wiki/index.php?title=Team:Paris_Bettencourt/Suicide&diff=294714&oldid=prevAleksandra: /* Results */2012-10-27T01:33:17Z<p><span class="autocomment">Results</span></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>In conclusion, these results show that colicin produced by cells prevents the growth or even kills sensitive cells, whereas the cells expressing the immunity protein survive.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>In conclusion, these results show that colicin produced by cells prevents the growth or even kills sensitive cells, whereas the cells expressing the immunity protein survive.</div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"></ins></div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>====Persperctives====</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>====Persperctives====</div></td></tr>
</table>Aleksandrahttp://2012.igem.org/wiki/index.php?title=Team:Paris_Bettencourt/Suicide&diff=294702&oldid=prevAleksandra: /* Results */2012-10-27T01:32:59Z<p><span class="autocomment">Results</span></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Growth curves of cells with or without protein extract of colicin producing cells.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Growth curves of cells with or without protein extract of colicin producing cells.</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>We compare the growth of cells that have immunity protein or not. Colicin-containing protein extract significantly impedes the growth of cells that do not have immunity (green curve), whereas it does not affect cells expressing immunity (blue curve), and they grow as well as positive controls (red and purple lines, respectively).</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>We compare the growth of cells that have immunity protein or not. Colicin-containing protein extract significantly impedes the growth of cells that do not have immunity (green curve), whereas it does not affect cells expressing immunity (blue curve), and they grow as well as positive controls (red and purple lines, respectively).</div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"></ins></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Remarks: Immune cells grow even better in the presence of the colicin extract than in the presence of the buffer. This can be explained by the fact that the buffer used for protein extraction leads to cell lysis. In future, we will use the extract of cells that do not produce colicin, as a positive control. Also, in this setup, the step-like trend of the growth curve for the sensitive cells in the presence of colicin extract (blue) can be explained by the degradation of the colicin, and/or by the fact that it all colicin present could be used up at some point, and not affect further growth of cells. </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Remarks: Immune cells grow even better in the presence of the colicin extract than in the presence of the buffer. This can be explained by the fact that the buffer used for protein extraction leads to cell lysis. In future, we will use the extract of cells that do not produce colicin, as a positive control. Also, in this setup, the step-like trend of the growth curve for the sensitive cells in the presence of colicin extract (blue) can be explained by the degradation of the colicin, and/or by the fact that it all colicin present could be used up at some point, and not affect further growth of cells. </div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;">In conclusion, these results show that colicin produced by cells prevents the growth or even kills sensitive cells, whereas the cells expressing the immunity protein survive.</del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline"></div></del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">In conclusion, these results show that colicin produced by cells prevents the growth or even kills sensitive cells, whereas the cells expressing the immunity protein survive.</ins></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>====Persperctives====</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>====Persperctives====</div></td></tr>
</table>Aleksandrahttp://2012.igem.org/wiki/index.php?title=Team:Paris_Bettencourt/Suicide&diff=294672&oldid=prevAleksandra: /* Results */2012-10-27T01:32:14Z<p><span class="autocomment">Results</span></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[[Image:PB2012_Immunity_exp.jpeg|700px|center]]</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[[Image:PB2012_Immunity_exp.jpeg|700px|center]]</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Growth curves of cells with or without protein extract of colicin producing cells.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Growth curves of cells with or without protein extract of colicin producing cells.</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>We compare the growth of cells that have immunity protein or not.</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>We compare the growth of cells that have immunity protein or not. <ins class="diffchange diffchange-inline">Colicin-containing protein extract significantly impedes the growth of cells that do not have immunity (green curve), whereas it does not affect cells expressing immunity (blue curve), and they grow as well as positive controls (red and purple lines, respectively).</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">Remarks: Immune cells grow even better in the presence of the colicin extract than in the presence of the buffer. This can be explained by the fact that the buffer used for protein extraction leads to cell lysis. In future, we will use the extract of cells that do not produce colicin, as a positive control. Also, in this setup, the step-like trend of the growth curve for the sensitive cells in the presence of colicin extract (blue) can be explained by the degradation of the colicin, and/or by the fact that it all colicin present could be used up at some point, and not affect further growth of cells. </ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">In conclusion, these results show that colicin produced by cells prevents the growth or even kills sensitive cells, whereas the cells expressing the immunity protein survive.</ins></div></td></tr>
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</table>Aleksandrahttp://2012.igem.org/wiki/index.php?title=Team:Paris_Bettencourt/Suicide&diff=292203&oldid=prevJrieders: /* Related Parts and Links */2012-10-26T23:40:32Z<p><span class="autocomment">Related Parts and Links</span></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*[http://partsregistry.org/wiki/index.php/Part:BBa_J23108 BBa_J23108] Anderson Constitutive Promoter with relative strength 0.51 (RFP reporter)</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*[http://partsregistry.org/wiki/index.php/Part:BBa_J23108 BBa_J23108] Anderson Constitutive Promoter with relative strength 0.51 (RFP reporter)</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*[http://partsregistry.org/wiki/index.php/Part:BBa_J23102 BBa_J23102] Anderson Constitutive Promoter with relative strength 0.86 (RFP reporter)</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*[http://partsregistry.org/wiki/index.php/Part:BBa_J23102 BBa_J23102] Anderson Constitutive Promoter with relative strength 0.86 (RFP reporter)</div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">*[http://partsregistry.org/Part:BBa_K914016 K914016] coding sequence of Colicin E2</ins></div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==References==</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==References==</div></td></tr>
</table>Jriedershttp://2012.igem.org/wiki/index.php?title=Team:Paris_Bettencourt/Suicide&diff=292124&oldid=prevJrieders: /* Discussion */2012-10-26T23:36:31Z<p><span class="autocomment">Discussion</span></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>To see more details on the toxicity assays, click [http://openwetware.org/wiki/IGEM:Paris_Bettencourt_2012/Notebooks/suicide_group/day_by_day//2012/09/08 here]</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>To see more details on the toxicity assays, click [http://openwetware.org/wiki/IGEM:Paris_Bettencourt_2012/Notebooks/suicide_group/day_by_day//2012/09/08 here]</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>We <del class="diffchange diffchange-inline">will continue on our quest to clone </del>the colicin E2 activity protein, and <del class="diffchange diffchange-inline">hope that future teams can learn from our experience</del>.</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">'''UPDATE:'''</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>We <ins class="diffchange diffchange-inline">are still struggling with cloning </ins>the colicin E2 activity protein, <ins class="diffchange diffchange-inline">however we have managed to clone the coding sequence </ins>and <ins class="diffchange diffchange-inline">have some more promising results using the sRNA approach described [https://2012.igem</ins>.<ins class="diffchange diffchange-inline">org/Team:Paris_Bettencourt/Delay here]</ins></div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==Related Parts and Links==</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==Related Parts and Links==</div></td></tr>
</table>Jriedershttp://2012.igem.org/wiki/index.php?title=Team:Paris_Bettencourt/Suicide&diff=291979&oldid=prevGVillain: /* Testing the effects of colicin-containing protein extract on E.coli */2012-10-26T23:28:50Z<p><span class="autocomment">Testing the effects of colicin-containing protein extract on E.coli</span></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>====Results====</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>====Results====</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[[Image:PB2012_Immunity_exp.jpeg|700px|center]]</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[[Image:PB2012_Immunity_exp.jpeg|700px|center]]</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;"></div></del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Growth curves of cells with or without protein extract of colicin producing cells.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Growth curves of cells with or without protein extract of colicin producing cells.</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>We compare the growth of cells that have immunity protein or not.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>We compare the growth of cells that have immunity protein or not.</div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"></div></ins></div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>====Persperctives====</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>====Persperctives====</div></td></tr>
</table>GVillainhttp://2012.igem.org/wiki/index.php?title=Team:Paris_Bettencourt/Suicide&diff=291966&oldid=prevGVillain: /* Results */2012-10-26T23:27:41Z<p><span class="autocomment">Results</span></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div></div></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div></div></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">Growth curves of cells with or without protein extract of colicin producing cells.</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">We compare the growth of cells that have immunity protein or not.</ins></div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>====Persperctives====</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>====Persperctives====</div></td></tr>
</table>GVillainhttp://2012.igem.org/wiki/index.php?title=Team:Paris_Bettencourt/Suicide&diff=291843&oldid=prevJeancury: /* Experiments and results */2012-10-26T23:19:55Z<p><span class="autocomment">Experiments and results</span></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Therefore we still need to improve protocol to provide a more reliable quantitative data.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Therefore we still need to improve protocol to provide a more reliable quantitative data.</div></td></tr>
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<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"><div id="boston"></ins></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>===Testing the effects of colicin-containing protein extract on E.coli===</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>===Testing the effects of colicin-containing protein extract on E.coli===</div></td></tr>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>[[Image:PB2012_Immunity_exp.jpeg |<del class="diffchange diffchange-inline">600px</del>]]</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>[[Image:PB2012_Immunity_exp.jpeg|<ins class="diffchange diffchange-inline">700px|center</ins>]]</div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>====Persperctives====</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>====Persperctives====</div></td></tr>
</table>Jeancuryhttp://2012.igem.org/wiki/index.php?title=Team:Paris_Bettencourt/Suicide&diff=291491&oldid=prevGVillain: /* Testing the effects of colicin-containing protein extract on E.coli */2012-10-26T23:00:50Z<p><span class="autocomment">Testing the effects of colicin-containing protein extract on E.coli</span></p>
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<td colspan='2' style="background-color: white; color:black;">Revision as of 23:00, 26 October 2012</td>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>We mix <del class="diffchange diffchange-inline">them </del>with the supernatant of centrifuged Colicin cells (<del class="diffchange diffchange-inline">only </del>the ColE2 protein). Therefore we can put the antibiotic during the whole experiment to avoid plasmid loss.</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>We mix <ins class="diffchange diffchange-inline">Z1 cells </ins>with the supernatant of centrifuged Colicin cells (<ins class="diffchange diffchange-inline">exctract with </ins>the ColE2 protein). Therefore we can put the antibiotic during the whole experiment to avoid plasmid loss.</div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>====Experimental Setup====</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>====Experimental Setup====</div></td></tr>
</table>GVillainhttp://2012.igem.org/wiki/index.php?title=Team:Paris_Bettencourt/Suicide&diff=291445&oldid=prevGVillain: /* Results */2012-10-26T22:58:05Z<p><span class="autocomment">Results</span></p>
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<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">[[Image:PB2012_Immunity_exp.jpeg |600px]]</ins></div></td></tr>
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</table>GVillain