Materials:

• 10 mM KOAc pH 7.0 (10mL of 1M stock/L)
• 80 mM CaCl2 x 2 H2O (11.8 g/L)
• 20 mM MnCl2 x 4 H2O (4.0 g/L)
• 10 mM MgCl2 x 6 H2O (2.0 g/L)
• 10% Glycerol (100 mL/L)

Procedure:

• Adjust pH down to 6.4 with .01N HCl if necessary
• Sterile filter and store at 4C

Notes:

• Adjusting pH up may precipitate MnO2 from the Mn containing solutions
• Slight dark precipitate will not impede function of solution

Materials:

• 10 g Tryptone
• 5 g Yeast Extract
• 10 g NaCl
• 10 g of Agar (for 1% Agar Plates (w/v))
• 1 L ddH2O
• Antibiotic Stock (if Required)

Notes:

• Do not store, the gel will condense and become unusable
• Add the Antibiotic stock after pouring some non-antibiotic LB Plates
• 1 L Should pour ~40 Plates

Materials:

• 0.5% (w/v) Yeast Extract (5 g/L)
• 2% (w/v) Tryptone (20 g/L)
• 10 mM NaCl (.584 g/L)
• 2.5 mM KCl (.186 g/L)
• 20 mM MgSO4 (2.4 g/L)

Notes:

• Autoclave and Store at 4C