Team:Nanjing China Bio/01

Community Outreach

向人们介绍合成生物学、iGEM以及我们的工作
金陵中学交流活动
9月18日，来自我们iGEM团队的志愿者前往位于南京市中心的金陵中学进行了宣讲，向中学生们普及了一些关于分子生物学、合成生物学的基本知识，并向他们介绍了如何通过分子生物学的实验来改造沙门氏菌，以使得沙门氏菌能够具有更强的靶向性和更弱的毒性以使之适用于癌症的临床治疗。之后，我们还向他们介绍了iGEM高中部的相关部分which they show great interest in，我们鼓励他们参与iGEM高中部的竞赛并表示愿意提供力所能及帮助。在宣讲结束后，我们与高中生进行了亲切的交流。他们问了我们许多关于生物学方面的问题，尽管不是每个问题都与我们的工作紧密相关的。
东南大学交流活动
9月20日，我们团队中的一些队员前往东南大学宣讲我们的工作。因为在暑假期间我们与东南大学的iGEM团队有过合作及联谊活动，因此他们对我们的工作比较了解，在我们宣讲的过程中提出了很多有意义的问题与建议。活动过程中，我们主要向他们介绍了细菌疗法优于常规方法的几个特征，诸如（从背景上摘几个）。在交流时间，他们为我们提供了很多关于实验方法的建，并就细菌治疗安全性的问题进行了深入的讨论。
南京大学宣讲活动
9月21日，我们在南大仙林校区向生科院的同学和老师们介绍了我们的活动内容。宣讲过程中我们介绍了所用菌株VBP20009应用于癌症治疗的优点，以及优化改菌株的具体方法，即通过筛选营养缺陷型菌株和转入厌氧启动子的方法提高厌氧靶向性和溶癌性。最后，我们对细菌厌氧靶向性疗法的未来进行了展望。老师和同学们给与我们的工作以高度的评价，并提供和一些建设性建议。

Community Outreach
Introduce the concept of synthetic biology, the principle of iGEM and our latest work to the community.
Lecture in Jinling High School
On the afternoon of 18th September, the volunteers of our iGEM team gave a lecture at Jinling High School in downtown Nanjing. We introduced the basic knowledge of molecular and synthetic biology to the students, and helped them comprehend how to modify Salmonella Typhimurium through molecular experiments to increase their ability of tumor targeting and decrease their toxicity in order to be suitable for clinical treatments. We later presented iGEM High School Division part which they showed great interest in. We encouraged them to take part in the division and would like to help them with all our efforts. After the lecture, we had a great time talking with the students. They were curious about the biology world and asked many questions related to our work.
Communication with Southeast University (SEU)
On 20th September, 3 members of our team went to SEU and had a seminar with students there. They knew our work quite well because we had cooperation with the iGEM team of SEU during the summer vacation. We mainly introduced some characteristics of bacteria therapy from other normal therapies like high colonization and capability of carrying multiple exogenous genes of bacteria. During the seminar, they raised many valuable questions and offered many suggestions on our experiment methods. We further discussed on the safety problems of bacteria therapy and came up with some ideas trying to solve them.
Lecture in Nanjing University
On 21st September, we presented our work to the teachers and students of School of Life Science in Nanjing University on Xianlin Campus. We introduced the advantages for VNP20009, the bacteria we used, to be applied in bacteria treatment and the detailed method to optimize the bacteria, which is to screen auxotrophic strains and transfect anaerobic promoters into the bacteria to enhance their efficiency of tumor targeting and killing cancer cells. In the end of our lecture, we made our outlook on the future of tumor targeting therapy with bacteria. The professors gave high praises on our work and offered many constructive comments.