Team:Nanjing-China/Safety

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Key questions
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     <li><strong>Would any of your project ideas raise safety issues in terms of:
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        <li> researcher safety, </li>
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        <li> public safety, or </li>
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        <li> environmental safety? </li>
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    <li><strong> Do any of the new BioBrick parts (or devices) that you made this year raise any safety issues? If yes,
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        <li> did you document these issues in the Registry? </li>
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        <li> how did you manage to handle the safety issue? </li>
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        <li> How could other teams learn from your experience? </li>
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     <li><strong>Is there a local biosafety group, committee, or review board at your institution? </strong>
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            <li> If yes, what does your local biosafety group think about your project? </li>
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            <li> If no, which specific biosafety rules or guidelines do you have to consider in your country? </li>
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        </ul>
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    </li>
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    <li><strong>Do you have any other ideas how to deal with safety issues that could be useful for future iGEM competitions? How could parts, devices and systems be made even safer through biosafety engineering? </strong></li>
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  <td><p>&nbsp;</p>    </td>
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Teams, please document any answers to these safety questions on your wiki safety page. Judges will be asked to evaluate your project, in part, on the basis of if and how you considered and addressed issues of biological safety. If any questions arise regarding iGEM and biological safety please send an email to safety AT igem.org.  
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          <h2>Abstract</h2>
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          <p>We further developed "Dr. E. coli": our project of iGEM 2010. Last year, we showed that Type 3 Secretion System (T3SS) works in E. coli by injecting GFP into RK13 cells. T3SS is a syringe like organelle found in bacterium such as Salmonella which uses it to inject virulence effector proteins into a target eukaryotic cell. We think this system can be applied to direct reprogramming of somatic cells among many other things.</p>
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          <p>We tested T3SS performance and tried to make it more convenient. For this purpose we designed a plasmid backbone which can instantly produce ready-to-inject fusion proteins from ordinary biobrick part. Using it, we tried to further characterize this system by injecting a library of protein domains.</p>
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          <p>In Science Gallery we exhibited awesome photographs related with biotechnology in public. We tried to catch the pedestrians’ interest in current synthetic biology and explore their thoughts.</p>
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Revision as of 14:44, 17 September 2012

NanjingChina

Abstract

We further developed "Dr. E. coli": our project of iGEM 2010. Last year, we showed that Type 3 Secretion System (T3SS) works in E. coli by injecting GFP into RK13 cells. T3SS is a syringe like organelle found in bacterium such as Salmonella which uses it to inject virulence effector proteins into a target eukaryotic cell. We think this system can be applied to direct reprogramming of somatic cells among many other things.

We tested T3SS performance and tried to make it more convenient. For this purpose we designed a plasmid backbone which can instantly produce ready-to-inject fusion proteins from ordinary biobrick part. Using it, we tried to further characterize this system by injecting a library of protein domains.

In Science Gallery we exhibited awesome photographs related with biotechnology in public. We tried to catch the pedestrians’ interest in current synthetic biology and explore their thoughts.

Retrieved from "http://2012.igem.org/Team:Nanjing-China/Safety"