Team:Nanjing-China/Project

From 2012.igem.org

(Difference between revisions)
(Prototype team page)
Line 1: Line 1:
-
<!-- *** What falls between these lines is the Alert Box!  You can remove it from your pages once you have read and understood the alert *** -->
+
<html lang="en">   
 +
    <!-- Le styles for debug
 +
    <link rel="stylesheet/less" type="text/css" href="less/page-index.less">
 +
    <script src="less/less-1.3.0.min.js" type="text/javascript"></script>-->
-
<html>
+
    <link rel="stylesheet" type="text/css" href="http://note.woku1.com/images/css/style.css">
-
<div id="box" style="width: 700px; margin-left: 137px; padding: 5px; border: 3px solid #000; background-color: #fe2b33;">
+
-
<div id="template" style="text-align: center; font-weight: bold; font-size: large; color: #f6f6f6; padding: 5px;">
+
-
This is a template page. READ THESE INSTRUCTIONS.
+
-
</div>
+
-
<div id="instructions" style="text-align: center; font-weight: normal; font-size: small; color: #f6f6f6; padding: 5px;">
+
-
You are provided with this team page template with which to start the iGEM season.  You may choose to personalize it to fit your team but keep the same "look." Or you may choose to take your team wiki to a different level and design your own wiki.  You can find some examples <a href="https://2008.igem.org/Help:Template/Examples">HERE</a>.
+
-
</div>
+
-
<div id="warning" style="text-align: center; font-weight: bold; font-size: small; color: #f6f6f6; padding: 5px;">
+
-
You <strong>MUST</strong> have all of the pages listed in the menu below with the names specified.  PLEASE keep all of your pages within your teams namespace. 
+
-
</div>
+
-
</div>
+
-
</html>
+
-
<!-- *** End of the alert box *** -->
+
    <!-- Le HTML5 shim, for IE6-8 support of HTML5 elements -->
 +
    <!--[if lt IE 9]>
 +
      <script src="http://html5shim.googlecode.com/svn/trunk/html5.js"></script>
 +
    <![endif]-->
 +
    <!-- Le fav and touch icons -->
 +
    <link rel="shortcut icon" href="ico/favicon.ico">
 +
    <link rel="apple-touch-icon-precomposed" sizes="144x144" href="ico/apple-touch-icon-144-precomposed.png">
 +
    <link rel="apple-touch-icon-precomposed" sizes="114x114" href="ico/apple-touch-icon-114-precomposed.png">
 +
    <link rel="apple-touch-icon-precomposed" sizes="72x72" href="ico/apple-touch-icon-72-precomposed.png">
 +
    <link rel="apple-touch-icon-precomposed" href="ico/apple-touch-icon-57-precomposed.png">
-
{| style="color:#1b2c8a;background-color:#0c6;" cellpadding="3" cellspacing="1" border="1" bordercolor="#fff" width="62%" align="center"
+
    <script type="text/javascript">
-
!align="center"|[[Team:Nanjing-China|Home]]
+
-
!align="center"|[[Team:Nanjing-China/Team|Team]]
+
-
!align="center"|[https://igem.org/Team.cgi?year=2012&team_name=Nanjing-China Official Team Profile]
+
-
!align="center"|[[Team:Nanjing-China/Project|Project]]
+
-
!align="center"|[[Team:Nanjing-China/Parts|Parts Submitted to the Registry]]
+
-
!align="center"|[[Team:Nanjing-China/Modeling|Modeling]]
+
-
!align="center"|[[Team:Nanjing-China/Notebook|Notebook]]
+
-
!align="center"|[[Team:Nanjing-China/Safety|Safety]]
+
-
!align="center"|[[Team:Nanjing-China/Attributions|Attributions]]
+
-
|}
+
 +
    </script>
 +
    <style type="text/css">
 +
     
 +
    </style>
 +
    <!-- Navbar
 +
    ===================================================-->
 +
    <div class="navbar navbar-fixed-top">
 +
      <div class="navbar-inner">
 +
        <div class="container">
 +
          <button type="button" class="btn btn-navbar" data-toggle="collapse" data-target=".nav-collapse">
 +
            <span class="icon-bar"></span>
 +
            <span class="icon-bar"></span>
 +
            <span class="icon-bar"></span>
 +
          </button>
 +
          <a class="brand" href="./index.html">NanjingChina</a>
 +
          <div class="nav-collapse collapse">
 +
            <ul class="nav">
 +
              <li class="active">
 +
                <a class="home-link" href="https://2012.igem.org/Team:Nanjing-China">Home</a>
 +
              </li>
 +
              <li class="">
 +
                <a class="team-link" href="https://2012.igem.org/Team:Nanjing-China/Team">Team</a>
 +
              </li>
 +
              <li class="">
 +
                <a class="profile-link" href="https://igem.org/Team.cgi?year=2012&team_name=Nanjing-China">Profile</a>
 +
              </li>
 +
              <li class="">
 +
                <a class="project-link" href="https://2012.igem.org/Team:Nanjing-China/Project">Project</a>
 +
              </li>
 +
              <li class="">
 +
                <a class="parts-link" href="https://2012.igem.org/Team:Nanjing-China/Parts">Parts</a>
 +
              </li>
 +
              <li class="">
 +
                <a class="modeling-link" href="./less.html">Modeling</a>
 +
              </li>
 +
              <li class="divider-vertical"></li>
 +
              <li class="">
 +
                <a class="notebook-link" href="https://2012.igem.org/Team:Nanjing-China/Notebook">Notebook</a>
 +
              </li>
 +
              <li class="">
 +
                <a class="safety-link" href="https://2012.igem.org/Team:Nanjing-China/Safety">Safety</a>
 +
              </li>
 +
              <li class="">
 +
                <a class="attr-link" href="https://2012.igem.org/Team:Nanjing-China/Attributions">Attributions</a>
 +
              </li>
 +
            </ul>
 +
          </div>
 +
        </div>
 +
      </div>
 +
    </div><!--/navbar-->
 +
    <div class="container">
 +
      <div class="row">
 +
        <div class="span3">
 +
          <div class="well sidebar-nav">
 +
            <ul class="nav nav-list">
 +
              <li class="nav-header">Sidebar</li>
 +
              <li class="active"><a href="#">Link</a></li>
 +
              <li><a href="#">Link</a></li>
 +
              <li><a href="#">Link</a></li>
 +
              <li><a href="#">Link</a></li>
 +
              <li class="nav-header">Sidebar</li>
 +
              <li><a href="#">Link</a></li>
 +
              <li><a href="#">Link</a></li>
 +
              <li><a href="#">Link</a></li>
 +
              <li><a href="#">Link</a></li>
 +
              <li><a href="#">Link</a></li>
 +
              <li><a href="#">Link</a></li>
 +
              <li class="nav-header">Sidebar</li>
 +
              <li><a href="#">Link</a></li>
 +
              <li><a href="#">Link</a></li>
 +
              <li><a href="#">Link</a></li>
 +
            </ul>
 +
          </div>
 +
        </div><!--Sidebar content-->
 +
        <div class="span9">
 +
          <div class="contain-inner well">
 +
          <h2>Abstract</h2>
 +
          <p>We further developed "Dr. E. coli": our project of iGEM 2010. Last year, we showed that Type 3 Secretion System (T3SS) works in E. coli by injecting GFP into RK13 cells. T3SS is a syringe like organelle found in bacterium such as Salmonella which uses it to inject virulence effector proteins into a target eukaryotic cell. We think this system can be applied to direct reprogramming of somatic cells among many other things.</p>
 +
          <p>We tested T3SS performance and tried to make it more convenient. For this purpose we designed a plasmid backbone which can instantly produce ready-to-inject fusion proteins from ordinary biobrick part. Using it, we tried to further characterize this system by injecting a library of protein domains.</p>
 +
          <p>In Science Gallery we exhibited awesome photographs related with biotechnology in public. We tried to catch the pedestrians’ interest in current synthetic biology and explore their thoughts.</p>
 +
        </div>
 +
        </div><!--Body content-->
 +
      </div>
 +
    </div><!--/container-->
 +
    <!-- Footer
 +
    =================================================  -->
 +
    <footer class="footer">
 +
      <div class="wrapper outer">
 +
        <div class="wrapper inner">
 +
        </div>
 +
      </div>
 +
    </footer>
-
== '''Overall project''' ==
 
-
Tell us more about your project.  Give us background.  Use this is the abstract of your project. Be descriptive but concise (1-2 paragraphs)
+
    <!-- Le javascript
 +
    ================================================== -->
 +
    <!-- Placed at the end of the document so the pages load faster -->
 +
    <script src="http://ajax.googleapis.com/ajax/libs/jquery/1.7/jquery.min.js"></script>
 +
    <script>
 +
    window.jQuery || document.write('<script src="js/jquery-1.7.1.min.js"><\/script>')
 +
    </script>
 +
    <script src="http://note.woku1.com/images/js/bootstrap-collapse.js" type="text/javascript"></script>
-
 
+
    <!-- Analytics
-
 
+
    ================================================== -->
-
 
+
    <script>
-
 
+
    </script>
-
 
+
</html>
-
 
+
-
== Project Details==
+
-
 
+
-
 
+
-
 
+
-
 
+
-
 
+
-
=== Part 2 ===
+
-
 
+
-
 
+
-
 
+
-
 
+
-
 
+
-
=== The Experiments ===
+
-
 
+
-
 
+
-
 
+
-
 
+
-
=== Part 3 ===
+
-
 
+
-
 
+
-
 
+
-
 
+
-
== Results ==
+

Revision as of 14:39, 17 September 2012

Abstract

We further developed "Dr. E. coli": our project of iGEM 2010. Last year, we showed that Type 3 Secretion System (T3SS) works in E. coli by injecting GFP into RK13 cells. T3SS is a syringe like organelle found in bacterium such as Salmonella which uses it to inject virulence effector proteins into a target eukaryotic cell. We think this system can be applied to direct reprogramming of somatic cells among many other things.

We tested T3SS performance and tried to make it more convenient. For this purpose we designed a plasmid backbone which can instantly produce ready-to-inject fusion proteins from ordinary biobrick part. Using it, we tried to further characterize this system by injecting a library of protein domains.

In Science Gallery we exhibited awesome photographs related with biotechnology in public. We tried to catch the pedestrians’ interest in current synthetic biology and explore their thoughts.