Team:NYMU-Taipei/ymis5.html

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Revision as of 16:34, 17 October 2012

NYMU iGEM

Practical Application in Industrial Waste Detection

To further practical use, we believe our artificial creatures have high ability to digest H2S from industrial waste and keep our environment clean. Also, it’s a good way to evaluate whether these sulfite reductase gene have high potential to expressed on cyanobacteria or not. We cooperate with Chung Hwa Pulp Corporation and get some industrial waste water and gas. By Co-culture with our Cys I and Dsr expressing E.coli for 24hr and 48 hr, we predict that the concentration of H2S will decrease dramatically. Both gas phase and water phase of each group are analysed by Microvolume turbidimetry method (mentioned above). Finally, the results are as the same as we imagined. In other word, the H2S concentration decrease in both gas phase and water phase. We verify that our gene, Cys I and Dsr work again!!


Our artificial cyanobacteria and E.coli cleaning the industrial waste produced by Chung Hwa Pulp Corporation



Simplily co-culture the waste gas and water with our modified cyanobacteria and E.coli, we can clean the pollution


In waste gas removal experiment, our pTrc-kan-Cys I and pTrc-kan-Dsr transformed E.coli have powerful ability to convert sulfite gas waste into H2S in both water and gas layer, compared to blank control pTrc-kan transformed E.coli





In waste water removal experiment, our pTrc-kan-Cys I and pTrc-kan-Dsr transformed E.coli also can convert sulfite water waste into H2S in both water and gas layer, compared to blank control pTrc-kan