Team:NYMU-Taipei/ymiq2.html

From 2012.igem.org

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     DCMU structure and its mechanism on photosynthesis<br />
     DCMU structure and its mechanism on photosynthesis<br />
     http://en.wikipedia.org/wiki/File:Diuron.png <br />
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     <p>From the previous  studies, it is suggested that <em>Synechococcus  SP. PCC 7002 </em>is able to metabolize sulfide compounds. We took advantage of  the results in our last experiment and adjusted the concentration of DCMU to an  appropriate degree. Since sulfide would become the main reducing energy for photoassimilation  under the effect of DCMU, we believe the more sulfide concentration in the  wells, the better cell growth would be observed.</p>
     <p>From the previous  studies, it is suggested that <em>Synechococcus  SP. PCC 7002 </em>is able to metabolize sulfide compounds. We took advantage of  the results in our last experiment and adjusted the concentration of DCMU to an  appropriate degree. Since sulfide would become the main reducing energy for photoassimilation  under the effect of DCMU, we believe the more sulfide concentration in the  wells, the better cell growth would be observed.</p>
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Revision as of 09:43, 18 October 2012

NYMU iGEM

Methods

Resistance of Synechococcus SP. PCC 7002 to 3 - (3,4-dichlorophenyl) - 1,1 – dimethylurea (DCMU)

From the previous research, we discovered that the concentration of 3 - (3,4 - dichlorophenyl) - 1,1 – dimethylurea (DCMU) must be adjusted to meet our requirement. Under certain DCMU concentration, the presence of sulfide would be extreme decisive condition which determines whether the colonies live or die. In this experiment, DCMU is diluted with A2 medium to explore the relationship between DCMU concentration and cell growth. Sodium sulfide is added to the experimental group and its initial concentration is controlled to 10 mM.


DCMU structure and its mechanism on photosynthesis
http://en.wikipedia.org/wiki/File:Diuron.png

Sodium sulfide concentration and cell growth

From the previous studies, it is suggested that Synechococcus SP. PCC 7002 is able to metabolize sulfide compounds. We took advantage of the results in our last experiment and adjusted the concentration of DCMU to an appropriate degree. Since sulfide would become the main reducing energy for photoassimilation under the effect of DCMU, we believe the more sulfide concentration in the wells, the better cell growth would be observed.