http://2012.igem.org/wiki/index.php?title=Team:NYMU-Taipei/ymip1.html&feed=atom&action=history
Team:NYMU-Taipei/ymip1.html - Revision history
2024-03-29T11:23:13Z
Revision history for this page on the wiki
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http://2012.igem.org/wiki/index.php?title=Team:NYMU-Taipei/ymip1.html&diff=287151&oldid=prev
Whimsical at 17:34, 26 October 2012
2012-10-26T17:34:26Z
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div> <a href="https://2012.igem.org/Team:NYMU-Taipei"><img src="https://static.igem.org/mediawiki/2012/<del class="diffchange diffchange-inline">7</del>/<del class="diffchange diffchange-inline">7d</del>/<del class="diffchange diffchange-inline">Ymi_header</del>.jpg" border="0"></a></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div> <a href="https://2012.igem.org/Team:NYMU-Taipei"><img src="https://static.igem.org/mediawiki/2012/<ins class="diffchange diffchange-inline">1</ins>/<ins class="diffchange diffchange-inline">15</ins>/<ins class="diffchange diffchange-inline">Ymi_header1</ins>.jpg" border="0"></a></div></td></tr>
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Whimsical
http://2012.igem.org/wiki/index.php?title=Team:NYMU-Taipei/ymip1.html&diff=271172&oldid=prev
Jayms at 04:37, 19 October 2012
2012-10-19T04:37:24Z
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>culturing</a></li></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>culturing</a></li></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <li><a title="Reprogramming of Somatic Cells into Stem &amp; Separation of iPS cells" </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <li><a title="Reprogramming of Somatic Cells into Stem &amp; Separation of iPS cells" </div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>href="https://2012.igem.org/Team:NYMU-Taipei/ymip3.html">Reprogramming of Somatic Cells into Stem <del class="diffchange diffchange-inline">Cell </del>&amp; Separation of iPS cells</a></li></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>href="https://2012.igem.org/Team:NYMU-Taipei/ymip3.html">Reprogramming of Somatic Cells into Stem &amp; Separation of iPS cells</a></li></div></td></tr>
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Jayms
http://2012.igem.org/wiki/index.php?title=Team:NYMU-Taipei/ymip1.html&diff=271150&oldid=prev
Jayms at 03:44, 19 October 2012
2012-10-19T03:44:11Z
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <li><a title="Reprogramming of Somatic Cells into Stem &amp; Separation of iPS cells" </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <li><a title="Reprogramming of Somatic Cells into Stem &amp; Separation of iPS cells" </div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>href="https://2012.igem.org/Team:NYMU-Taipei/ymip3.html">Reprogramming of Somatic Cells into Stem &amp; Separation of iPS cells</a></li></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>href="https://2012.igem.org/Team:NYMU-Taipei/ymip3.html">Reprogramming of Somatic Cells into Stem <ins class="diffchange diffchange-inline">Cell </ins>&amp; Separation of iPS cells</a></li></div></td></tr>
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Jayms
http://2012.igem.org/wiki/index.php?title=Team:NYMU-Taipei/ymip1.html&diff=271147&oldid=prev
Jayms at 03:41, 19 October 2012
2012-10-19T03:41:14Z
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <li><a title="Reprogramming of Somatic Cells into Stem &amp; Separation of iPS cells" </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <li><a title="Reprogramming of Somatic Cells into Stem &amp; Separation of iPS cells" </div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>href="https://2012.igem.org/Team:NYMU-Taipei/ymip3.html">Reprogramming of Somatic<del class="diffchange diffchange-inline"><br /></del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>href="https://2012.igem.org/Team:NYMU-Taipei/ymip3.html">Reprogramming of Somatic Cells into Stem &amp; Separation of iPS cells</a></li></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>Cells into Stem <del class="diffchange diffchange-inline">Cell </del>&amp; <del class="diffchange diffchange-inline"><br /></del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>Separation of iPS cells</a></li></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div></div></td></tr>
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Jayms
http://2012.igem.org/wiki/index.php?title=Team:NYMU-Taipei/ymip1.html&diff=271138&oldid=prev
Jayms at 03:33, 19 October 2012
2012-10-19T03:33:10Z
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <div class="text_area" align="justify"></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <div class="text_area" align="justify"></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><div class="title"><del class="diffchange diffchange-inline">Protocol</del></div></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><div class="title"><ins class="diffchange diffchange-inline">J774 Macrophage Cell Culturing</ins></div></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><div align="left"></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><div align="left"></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline"> <p><span class="subtitle">J774 Macrophage Cell Culturing</span></p></del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div> </div></td></tr>
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Jayms
http://2012.igem.org/wiki/index.php?title=Team:NYMU-Taipei/ymip1.html&diff=269986&oldid=prev
Jayms at 02:59, 16 October 2012
2012-10-16T02:59:44Z
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>href="https://2012.igem.org/Team:NYMU-Taipei/ymisf.html">Safety</a></li></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>href="https://2012.igem.org/Team:NYMU-Taipei/ymisf.html">Safety</a></li></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <li><a title="Collaboration with NTU" href="https://2012.igem.org/Team:NYMU-Taipei/ymico1.html">Collaboration with NTU</a></li></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <li><a title="Collaboration with NTU" href="https://2012.igem.org/Team:NYMU-Taipei/ymico1.html">Collaboration with NTU</a></li></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline"> <li><a title="Collaboration with Entrepreneur" href="https://2012.igem.org/Team:NYMU-Taipei/ymico2.html">Collaboration with<br /></del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div> </div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline">Entrepreneur</a></li></del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <li><a title="NYMU Bioenergy Breakthrough" href="https://2012.igem.org/Team:NYMU-Taipei/ymibt.html">NYMU Bioenergy<br /></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <li><a title="NYMU Bioenergy Breakthrough" href="https://2012.igem.org/Team:NYMU-Taipei/ymibt.html">NYMU Bioenergy<br /></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Breakthrough</a></li></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Breakthrough</a></li></div></td></tr>
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Jayms
http://2012.igem.org/wiki/index.php?title=Team:NYMU-Taipei/ymip1.html&diff=230560&oldid=prev
Jayms at 01:37, 27 September 2012
2012-09-27T01:37:03Z
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <li><a title="Collaboration with Entrepreneur" href="https://2012.igem.org/Team:NYMU-Taipei/ymico2.html">Collaboration with<br /></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <li><a title="Collaboration with Entrepreneur" href="https://2012.igem.org/Team:NYMU-Taipei/ymico2.html">Collaboration with<br /></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Entrepreneur</a></li></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Entrepreneur</a></li></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"> <li><a title="NYMU Bioenergy Breakthrough" href="https://2012.igem.org/Team:NYMU-Taipei/ymibt.html">NYMU Bioenergy<br /></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">Breakthrough</a></li></ins></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <li><a title="Parts" </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <li><a title="Parts" </div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>href="https://2012.igem.org/Team:NYMU-Taipei/ymiparts.html">Parts</a></li></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>href="https://2012.igem.org/Team:NYMU-Taipei/ymiparts.html">Parts</a></li></div></td></tr>
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Jayms
http://2012.igem.org/wiki/index.php?title=Team:NYMU-Taipei/ymip1.html&diff=229485&oldid=prev
Jayms at 01:16, 27 September 2012
2012-09-27T01:16:53Z
<p></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>href="https://2012.igem.org/Team:NYMU-Taipei/ymisf.html">Safety</a></li></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>href="https://2012.igem.org/Team:NYMU-Taipei/ymisf.html">Safety</a></li></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div> <li><a title="Collaboration" </div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div> <li><a title="Collaboration <ins class="diffchange diffchange-inline">with NTU</ins>" <ins class="diffchange diffchange-inline">href="https://2012.igem.org/Team:NYMU-Taipei/ymico1.html">Collaboration with NTU</a></li></ins></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>href="https://2012.igem.org/Team:NYMU-Taipei/<del class="diffchange diffchange-inline">ymico</del>.html">Collaboration</a></li></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline"> <li><a title="Collaboration with Entrepreneur" </ins>href="https://2012.igem.org/Team:NYMU-Taipei/<ins class="diffchange diffchange-inline">ymico2</ins>.html">Collaboration <ins class="diffchange diffchange-inline">with<br /></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">Entrepreneur</ins></a></li></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <li><a title="Parts" </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <li><a title="Parts" </div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>href="https://2012.igem.org/Team:NYMU-Taipei/ymiparts.html">Parts</a></li></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>href="https://2012.igem.org/Team:NYMU-Taipei/ymiparts.html">Parts</a></li></div></td></tr>
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Jayms
http://2012.igem.org/wiki/index.php?title=Team:NYMU-Taipei/ymip1.html&diff=217255&oldid=prev
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<a <br />
href="https://2012.igem.org/Team:NYMU-Taipei/ymiproject.html" target="_parent">Project Venusian</a> ·<br />
<a <br />
href="https://2012.igem.org/Team:NYMU-Taipei/ymim1" target="_parent">Modeling</a> · <br />
<a <br />
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<a <br />
href="https://2012.igem.org/Team:NYMU-Taipei/ymijf.html">Extras</a> · <br />
<a <br />
href="https://2012.igem.org/Team:NYMU-Taipei/ymit1">Team</a> · <br />
<a href="https://2012.igem.org/Main_Page">iGEM</a><br />
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<div class="title">Protocol</div><br />
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<p><span class="subtitle">J774 Macrophage Cell Culturing</span></p><br />
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<p><div class=out style='text-align:center'><img src="https://static.igem.org/mediawiki/2012/8/8f/Ymip1.gif" alt="" width="609" height="442" /></div></p><br />
<ol><br />
<li>J774 cell line, a murine macrophages cell line established from a tumor that arose in a female BALB/c mouse, were provided by Prof. Yi-Hsuan Lee from National Yang Ming University. </li><br />
<li>View cultures using a microscopy and after the cultures was full of the dish, remove the spent medium. </li><br />
<li>Wash the cell monolayer with PBS and remove it.</li><br />
<li>Add medium(1ml per 25 cm2 of surface area) and scrape cells down.</li><br />
<li>Ensure cells were detached by a microscopy.</li><br />
<li>Centrifuge under 2000 rpm, 5minutes, 25°C.</li><br />
<li>Remove the supernatant and add 5 ml medium to suspend cells.</li><br />
<li>Count the cells by Bright Line Counting Chamber. Subject the cell diversity to 104/ml.</li><br />
<li>Seed the cells for 104/well, 1ml/well in the 24-well plate.</li><br />
<li>Until cells grow full of each well, do the coculturing experiment.<br /><br />
</li></ol></div><br />
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<h2 class="drawer-handle">Protocol</h2><br />
<ul><br />
<li><a title="J774 macrophage cell line culturing" <br />
href="https://2012.igem.org/Team:NYMU-Taipei/ymip1.html">J774 macrophage cell <br /><br />
culturing</a></li><br />
<li><a title="Mesenchymal stem cells culturing" <br />
href="https://2012.igem.org/Team:NYMU-Taipei/ymip2.html">Mesenchymal stem cells<br /><br />
culturing</a></li><br />
<li><a title="Reprogramming of Somatic Cells into Stem &amp; Separation of iPS cells" <br />
href="https://2012.igem.org/Team:NYMU-Taipei/ymip3.html">Reprogramming of Somatic<br /><br />
Cells into Stem Cell &amp; <br /><br />
Separation of iPS cells</a></li><br />
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<h2 class="drawer-handle">Extras</h2><br />
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<li><a title="Achievements" <br />
href="https://2012.igem.org/Team:NYMU-Taipei/ymijf.html">Achievements</a></li><br />
<li><a title="Safety" <br />
href="https://2012.igem.org/Team:NYMU-Taipei/ymisf.html">Safety</a></li><br />
<li><a title="Collaboration" <br />
href="https://2012.igem.org/Team:NYMU-Taipei/ymico.html">Collaboration</a></li><br />
<li><a title="Parts" <br />
href="https://2012.igem.org/Team:NYMU-Taipei/ymiparts.html">Parts</a></li><br />
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<h2 class="drawer-handle">Notebook</h2><br />
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<li><a title="Week 1 ~ Week 4" <br />
href="https://2012.igem.org/Team:NYMU-Taipei/ymiw1.html">Week 1 ~ Week 4</a></li><br />
<li><a title="Week 5 ~ Week 8" <br />
href="https://2012.igem.org/Team:NYMU-Taipei/ymiw2.html">Week 5 ~ Week 8</a></li><br />
<li><a title="Week 9 ~ Week 11" <br />
href="https://2012.igem.org/Team:NYMU-Taipei/ymiw3.html">Week 9 ~ Week 11</a></li><br />
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