Team:NYMU-Taipei/ymin4.html

From 2012.igem.org

(Difference between revisions)
Line 86: Line 86:
<div align="left">
<div align="left">
-
   <p>Recently, the  search for biological nitrogen removal method from wastewaters and exhaust air  has come up with several promising methods; however, most of them just took  advantage of some special bacteria combined with industrial procedures. On the  contrary, our iGEM project aim to reduce nitrogen oxides and oxidize sulfide  compounds at the same time. <br />
+
   <p><span class="subtitle">Practical Application</span></p>
-
    <br />
+
-
During the  processes of denitrification, sulfide compounds and nitrate act as electron  donor and acceptor respectively. This reaction is also known as sulfide-driven  denitrification. Researchers have reported that E. coli can perform such  reaction when expresses sqr gene from <em>R.  capsulatus</em>. Herein, we enable certain type of cyanobacteria to take  advantage of sulfide and reduce nitrogen oxide compounds into nitrogen. The  BLAST result shows that sqr genes are homolog in <em>R. capsulatus</em> and <em>Synechocystis  sp. PCC 6803</em>. 
+
-
For  denitrification, we plan to get access to <em>Thiobacillus  denitrificans</em>, the well-known chemolithotrophic organisms. Nevertheless, we  later found it difficult to obtain the specific strain we need. According to  NCBI database, enzymes for denitrification such as nir, nor, nos share great  similarity between <em>Thiobacillus  denitrificans </em>and <em>Pseudomonas  aeruginosa PAO1</em>, so we adopted <em>P.  aeruginosa PAO1</em> instead and expressed the enzymes mentioned above in <em>Synechocystis sp. PCC 6803</em>.<br />
+
-
<br />
+
-
<div class=out style='text-align:center'>
+
-
  <img src="http://igem.org/wiki/images/6/6c/Ymin2.png" alt="" width="554" height="190" border="0" align="center"  /><br />
+
-
  <p><a href="http://www.genome.jp/kegg/pathway/map/map00910.html">http://www.genome.jp/kegg/pathway/map/map00910.html</a><br />
+
-
  </p>
+
</div>
</div>
-
<p><br />
+
<div align="left">
-
   These  denitrifying enzymes are functional under aerobic condition, yet like all  cyanobacteria, <em>Synechocystis sp. PCC 6803</em> produces oxygen during photosynthesis. Fortunately, when sulfide presents in the  environment and sqr is expressed, it will cease producing oxygen and use sulfide as an electron donor for carbon dioxide photoassimilation. Together with dsrI and dsrII enzymes from <em>Desulfovibrio desulfuricans</em>, our engineered organisms are capable of reducing three major oxides pollution – nitrogen, sulfur and carbon oxides. <br />
+
   <p>Moreover, our  project has been put into practice thanks to the cooperation with Chung Hwa  Pulp Corporation. The wastewater generated by Pulp factories contains enormous  sulfide compounds and nitrate, which bring the annoying odors as well as the  contamination to the local environment. Fortunately, our project seems to be  the solution to their problem. This also demonstrates the potential and  possibility of commercialized our project. On top of that, the engineered cyanobacteria can become the third endosymbiosis organelles with the help of division inhibitor, gene for invasion. After installing our designation into plants or even human cells as artificial organelles, we grant eukaryotes the  ability to survive in extreme environments as horrible as Venus in case the  space immigration is necessary on day.<br />
-
  <br />
+
</div>
-
</p>
+
<div align="left">
-
  <div class="out" style='text-align:center'>  
+
  <p><span class="subtitle">References</span></p>
-
    <p><img src="http://igem.org/wiki/images/a/ab/Ymin3.png" alt=""  border="0" align="center" /><br />
+
</div>
-
    </p>
+
<div align="left">
-
    <p><a href="http://fleasnobbery.blogspot.tw/2008/07/cyanobacteria.html">http://fleasnobbery.blogspot.tw/2008/07/cyanobacteria.html</a></p>
+
  <p><em>Sulfur-driven  autotrophic denitrification: diversity ,biochemistry, and engineering  applications</em> (1 September 2010) by Ming-Fei Shao  &amp; Tong Zhang &amp; Herbert Han-Ping Fang 
-
   </div>
+
  <p><em>Characterization  of the membranous denitrification enzymes nitritereductase (cytochrome cd1) and  copper-containing nitrous oxidereductase from Thiobacillus denitrificans </em>(30 October 1995) by Ursula H. Hole · Kai-Uwe Vollack · Walter G. Zumft ·Effi Eisenmann · Roman A. Siddiqui ·Bärbel Friedrich · Peter M. H. Kroneck</p>
-
   <p><br />
+
  <p><em>Autotrophic  denitrification for combined hydrogen sulfideremoval from biogas and  post-denitrification </em>by R. Kleerebezem and R.  Mendez (2002)</p>
-
</p></div>
+
   <p><em>Nitrous  oxide production and consumption: regulation of gene expression by  gas-sensitive transcription factors </em>(7 July 2012)  by Stephen Spiro</p>
 +
   <p><em>Mechanism  of Sulfide-Quinone Reductase Investigated Using Site-Directed Mutagenesis and  Sulfur Analysis</em> (6 August 2002)by Christoph  Griesbeck, Michael Schu¨tz, Thomas Scho¨dl, Stephan Bathe, Lydia Nausch, Nicola  Mederer, Martin Vielreicher, and Gu¨nter Hauska</p></div>
</div>
</div>
       </div>
       </div>

Revision as of 08:18, 16 October 2012

NYMU iGEM

Practical Application & References

Practical Application

Practical Application

Moreover, our project has been put into practice thanks to the cooperation with Chung Hwa Pulp Corporation. The wastewater generated by Pulp factories contains enormous sulfide compounds and nitrate, which bring the annoying odors as well as the contamination to the local environment. Fortunately, our project seems to be the solution to their problem. This also demonstrates the potential and possibility of commercialized our project. On top of that, the engineered cyanobacteria can become the third endosymbiosis organelles with the help of division inhibitor, gene for invasion. After installing our designation into plants or even human cells as artificial organelles, we grant eukaryotes the ability to survive in extreme environments as horrible as Venus in case the space immigration is necessary on day.

References

Sulfur-driven autotrophic denitrification: diversity ,biochemistry, and engineering applications (1 September 2010) by Ming-Fei Shao & Tong Zhang & Herbert Han-Ping Fang

Characterization of the membranous denitrification enzymes nitritereductase (cytochrome cd1) and copper-containing nitrous oxidereductase from Thiobacillus denitrificans (30 October 1995) by Ursula H. Hole · Kai-Uwe Vollack · Walter G. Zumft ·Effi Eisenmann · Roman A. Siddiqui ·Bärbel Friedrich · Peter M. H. Kroneck

Autotrophic denitrification for combined hydrogen sulfideremoval from biogas and post-denitrification by R. Kleerebezem and R. Mendez (2002)

Nitrous oxide production and consumption: regulation of gene expression by gas-sensitive transcription factors (7 July 2012) by Stephen Spiro

Mechanism of Sulfide-Quinone Reductase Investigated Using Site-Directed Mutagenesis and Sulfur Analysis (6 August 2002)by Christoph Griesbeck, Michael Schu¨tz, Thomas Scho¨dl, Stephan Bathe, Lydia Nausch, Nicola Mederer, Martin Vielreicher, and Gu¨nter Hauska