Team:NYMU-Taipei/ymim2

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Revision as of 03:02, 27 September 2012

Enzyme


Substrate


Kcat (min-1)


Km(mM)


Organism


Reference


Nitrate Reductase (Nap)


Nitrate


3480


0.112


Paracoccus pantotrophus


(1)


Nitrite Reductase (Nir)


Nitrite


480


0.053


Pseudomonas aeruginosa


(2)


Nitric Oxide Reductase (Nor)


Nitric Oxide


12660


0.06


Fusarium oxysporum


(3)


Nitrous Oxide Reductase (Nos)


Nitrous Oxide


16800


0.0056


Paracoccus denitrificans


(4)



Enzyme


Substrate


Vmax (mM /min-1)


Km(mM)


Organism


Reference


Nitrate Reductase (Nap)


Nitrate


27.885


0.47


Escherichia coli


(5)


Nitrite Reductase (Nfr)


Nitrite


46.14


0.022


Escherichia coli


(6)


Nitrite Reductase (Nir)


Nitrite


0.465


0.4


Escherichia coli


(7)



Parameter


Description


Value


Units


Reference




Copy Number in E.coli


300


Copy Number per Cell


Part Registry




Ptrc Promoter Strength


0.00003156


Polymerase per min


(10)(11)(12)




Degradation Rate of mRNA in E.coli


0.102


min-1


(8)




Degradation Rate of Enzyme in E.coli


0.0348


min-1


(8)




Translation rate in E.coli


720~1260


Amino Acids/min


(9)




Avogadaros' Number


6.02*


mmole-1


N/A




Cell Volume of E.coli


6.5*


liter


(18)




Translation rate of Nap


0.763


Number of mRNA/min


Estimated




Translation rate of Nir


0.96


Number of mRNA/min


Estimated




Translation rate of Nor


1.668


Number of mRNA/min


Estimated




Translation rate of Nos


1.602


Number of mRNA/min


Estimated


Parameter


Description


Value


Units


Reference




Copy Number in E.coli


300


Copy Number per Cell


Part Registry




Ptrc Promoter Strength


0.00003156


Polymerase per min


(8)(9)(10)




Degradation Rate of mRNA in E.coli


0.102


min-1


(11)




Degradation Rate of Enzyme in E.coli


0.0348


min-1


(11)




Translation rate in E.coli


720~1260


Amino Acids/min


(12)




Avogadaros' Number


6.02*


mmole-1


N/A




Cell Volume of E.coli


6.5*


liter


(13)




Translation rate of Nap


0.763


Number of mRNA/min


Estimated




Translation rate of Nir


0.96


Number of mRNA/min


Estimated




Translation rate of Nor


1.668


Number of mRNA/min


Estimated




Translation rate of Nos


1.602


Number of mRNA/min


Estimated




Reference



[1]Andrew J. GATES, David J. RICHARDSON, Julea N. BUTT(2008) Voltammetric characterization of the aerobic energy-dissipating nitrate reductase of Paracoccus pantotrophus : exploring the activity of a redox-balancing enzyme as a f unction of electrochemical potential. Biochem. J. (2008) 409, 159–168

[2]Serena Rinaldo, Francesca Cutruzzolà(2007) Nitrite Reductases in Denitrification. Biology of the Nitrogen Cycle 37-55

[3]Naoki Toritsuka, Hirofumi Shoun, Udai P. Singh, Sam-Yong Park , Tetsutaro Iizuka, Yoshitsugu Shiro(1997) Functional and structural comparison of nitric oxide reductases from denitrifying fungi Cylindrocarpon tonkinense and Fusarium oxysporum. Biochimica et Biophysica Acta 1338 1997 93–99

[4]Stuart W. Snyder, Thomas C. Hollocher(1987) Purification and some characteristics of nitrous oxide reductase from Paracoccus denitrificans. The Journal of Biology Chemistry VOl. 262, No.14. Issue of May 15. , pp. 6515-6525

[5] Robert A. LAZZARINI, Daniel E. Atkinson(1961) A Triphosphopyridine Nucleotide-specific Nitrite Reductase from Escherichia coli. The Journal of Biology Chemistry VOl. 236, No.12. December

[6] Michael W. W. AdamsS, Leonard E. Mortenson(1982) The Effect of Cyanide and Ferricyanide on the Activity of the Dissimilatory Nitrate Reductase of Escherichia coli. The Journal of Biology Chemistry VOl. 257, No.4. Issue of February 25, pp. 1791-1799

[7] Lewis M. Siegel, David C. Rueger, Michael J. Barber, Rick J. Krueger(1982) Escherichia coli Sulfite Reductase Hemoprotein Subuni. The Journal of Biology Chemistry VOl. 257, No.11. Issue of June 10, pp. 6343-6350

[8] Hsin-Ho Huang, Daniel Camsund, Peter Lindblad and Thorsten Heidorn(2010) Design and characterization of molecular tools for a Synthetic Biology approach towards developing cyanobacterial biotechnology. Nucleic Acids Research, Vol. 38, No. 8 2577–2593

[9] Michael Brunner, Hermann Bujard(1987) Promoter recognition and promoter strength in the Escherichia coli system. The EMBO Journal vol.6 no.10 pp.3139-3144

[10]Thomas A . Clarke , Paul C. Mills, Susie R. Poock,bJulea N. Butt, Myles R. Cheesman, Jeffrey A. Cole, Jay C. D. Hinton, Andrew M. Hemmings, Gemma Kemp, Christopher A . G . So¨derberg, Stephen Spiro, Jessica Van Wonderen, David J. Richardson(2008)Escherichia coli Cytochrome c Nitrite Reductase NrfA. Methods in Enzymology, Vol. 437 pp.64-77

[11] Kmalendu Nath, Arthur L. Koch(1971) Protein Degradation in Escherichia coli. The Journal of Biology Chemistry VOl. 246, No.22. Issue of November 25, pp. 6956-6967

[12]Thomas A . Clarke , Paul C. Mills, Susie R. Poock,bJulea N. Butt, Myles R. Cheesman, Jeffrey A. Cole, Jay C. D. Hinton, Andrew M. Hemmings, Gemma Kemp, Christopher A . G . So¨derberg, Stephen Spiro, Jessica Van Wonderen, David J. Richardson(2008)Escherichia coli Cytochrome c Nitrite Reductase NrfA. Methods in Enzymology, Vol. 437 pp.64-77

[13] Cell Volume Increase in Escherichia coli after Shifts to Richer Media, H. E. KUBITSCHEK ,Biological and Medical Research Division, Argonne National Laboratory,9700 South Cass Avenue, Argonne, Illinois 60439