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Conclusion & References

  1. We have successfully cloned zinTp into pSB1C3
  2. We have successfully cloned zinTp-GFP into pSB1C3
  3. We have characterized zinTp function. The expression of downstream gene will increase when added cadmium ion into LB medium, and the maximum transcription rate occurs around 60 min after added 200μM cadmium.
  4. We have successfully cloned mnt into pSB1C3
  5. We have successfully cloned smtA into pSB1C3
  6. We have characterized smtA function. When expressing smtA, it enhances E.coli tolerance to cadmium.


Sigel, A.; Sigel, H.; Sigel, R.K.O., ed. (2009). Metallothioneins and Related Chelators. Metal Ions in Life Sciences. Cambridge: RSC Publishing

Jianguo Shi, William P. Lindsay, James W. Huckle, Andrew P. Morby and Nigel J. Robinson. Cyanobacterial metallothionein gene expressed in Escherichia coli Metal-binding properties of the expressed protein. FEB.5 11081 Volume 303, number 2,3, 159-163

Sode et al. (1998) Construction of a marine cyanobacterial strain with increased heavy metal ion tolerance by introducing exogenic metallothionein gene. J Mar Biotechnol

ZHIQI HAO,1 SHAOLIN CHEN,1 AND DAVID B. WILSON1,2,  Cloning, Expression, and Characterization of Cadmium and Manganese Uptake Genes from Lactobacillus plantarum, APPLIED AND ENVIRONMENTAL MICROBIOLOGY,Nov. 1999, p.4746–4752 Vol. 65, No. 11

A. Paskarova, P. Ferianc, J. Kormanec, D. Homerova, A. Farewell, T. Nystrom. Regulation of yodA encoding a novel cadmium-induced protein in Escherichia coli, Microbiology (2002), 148, 3801–3811

Damien Arnoult, Ire`ne Tatischeff, Je´rome Estaquier, Mathilde Girard, Franck Sureau, Jean Pierre Tissier, Alain Grodet, Marc Dellinger, Francois Traincard,Axel Kahn,Jean-Claude Ameisen, and Patrice Xavier Petit. “On the Evolutionary Conservation of the Cell Death Pathway: Mitochondrial Release of an Apoptosis-inducing Factor during Dictyostelium discoideum Cell Death”, Molecular Biology of the Cell Vol. 12, 3016–3030, October 2001