Team:NYMU-Taipei/ymic4.html

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   <p align="center"><img  src="../../../Users/jaymes/Desktop/ymic13.png" width="600" height="220" /><br />
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   <p align="center"><img  src="http://2012.igem.org/wiki/images/a/a3/Ymic13.png" width="600" height="220" /><br />
We got the right plasmid which contained zinTp<br />
We got the right plasmid which contained zinTp<br />
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     <p>In this fig we could  see that before 60 min, the fluorescence level of zinTp+GFP steadily increased,  while BBa_I13522 (positive control) remained stable around 35000 au as time  gone by. At 60 min, zinTp reached its maximum transcription rate so the  fluorescence level stop increasing around 55000 au.</p><div class="out" style='text-align:center'><img src="../../../Users/jaymes/Desktop/ymic16.png" alt="" width="494" height="306" /><br />
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     <p>In this fig we could  see that before 60 min, the fluorescence level of zinTp+GFP steadily increased,  while BBa_I13522 (positive control) remained stable around 35000 au as time  gone by. At 60 min, zinTp reached its maximum transcription rate so the  fluorescence level stop increasing around 55000 au.</p><div class="out" style='text-align:center'><img src="http://2012.igem.org/wiki/images/a/a0/Ymic16.png" alt="" width="494" height="306" /><br />
       <strong>Fluorescence level at 15mins after Cd2+  added </strong><br />
       <strong>Fluorescence level at 15mins after Cd2+  added </strong><br />
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   <p><span class="subtitle">Cloning mnt into pSB1C3 plasmid</span><br />
   <p><span class="subtitle">Cloning mnt into pSB1C3 plasmid</span><br />
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       Mnt gene was successfully cloned into pSB1C3 plasmid<br />
       Mnt gene was successfully cloned into pSB1C3 plasmid<br />
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       smtA gene was successfully cloned into pSB1C3 plasmid<br />
       smtA gene was successfully cloned into pSB1C3 plasmid<br />
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         <p><span class="subtitle">Characterizing of smtA</span><br />
         <p><span class="subtitle">Characterizing of smtA</span><br />
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         <strong>Cadmium 0/200/500μM Medium</strong><br />
         <strong>Cadmium 0/200/500μM Medium</strong><br />
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       <p>This fig shows that smtA enhances the tolerance  level to cadmium ion. In wild type E.coli, OD595 differ from between  0/200/500μM cadmium concentration. E.coli hardly grow in 500μM cadmium medium. However, the E.coli which can express smtA seem to show  no significant different between 0 &amp; 200μM cadmium medium.</p>
       <p>This fig shows that smtA enhances the tolerance  level to cadmium ion. In wild type E.coli, OD595 differ from between  0/200/500μM cadmium concentration. E.coli hardly grow in 500μM cadmium medium. However, the E.coli which can express smtA seem to show  no significant different between 0 &amp; 200μM cadmium medium.</p>
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         <strong>8 hours after cadmium added</strong><br />
         <strong>8 hours after cadmium added</strong><br />
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       <p>OD of WT E.coli  decrease when cadmium concentration increase. The E.coli which can express smtA  seem to have stronger resistance to cadmium toxicity and the E.coli  concentration show no significant different from 0 to 200μM cadmium medium.</p>
       <p>OD of WT E.coli  decrease when cadmium concentration increase. The E.coli which can express smtA  seem to have stronger resistance to cadmium toxicity and the E.coli  concentration show no significant different from 0 to 200μM cadmium medium.</p>
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  <em>E.coli</em> which could express smtA growth better than wild type in 200μM Cd+2 medium <br />
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Obviously, the E.coli  which could express smtA growth better than wild type in 200μM cadmium medium. <br />
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                 <li><a title="Abstract" href="http://2012.igem.org/Team:NYMU-Taipei/ymiq1.html">Abstract</a></li>
                 <li><a title="Abstract" href="http://2012.igem.org/Team:NYMU-Taipei/ymiq1.html">Abstract</a></li>
                 <li><a title="Methods" href="http://2012.igem.org/Team:NYMU-Taipei/ymiq2.html">Methods</a></li>
                 <li><a title="Methods" href="http://2012.igem.org/Team:NYMU-Taipei/ymiq2.html">Methods</a></li>
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                 <li><a title="Measurements" href="http://2012.igem.org/Team:NYMU-Taipei/ymiq3.html">Measurements</a></li>
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                 <li><a title="Experiments" href="http://2012.igem.org/Team:NYMU-Taipei/ymiq3.html">Experiments</a></li>
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                 <li><a title="Results & References" href="http://2012.igem.org/Team:NYMU-Taipei/ymiq4.html">Results &amp; References</a></li>
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                 <li><a title="Results & References" href="http://2012.igem.org/Team:NYMU-Taipei/ymiq4.html">Results &amp; References</a></li><li><a title="Further Experiments after Asia Jamboree" href="http://2012.igem.org/Team:NYMU-Taipei/ymiq5.html">Further Experiments after Asia Jamboree</a></li>
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             </ul>
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Latest revision as of 01:50, 27 October 2012

NYMU iGEM

Results & Discussion

Cloning zinTp into pSB1C3 plasmid


We got the right plasmid which contained zinTp

Characterizing of zinTp





In this fig we could see that before 60 min, the fluorescence level of zinTp+GFP steadily increased, while BBa_I13522 (positive control) remained stable around 35000 au as time gone by. At 60 min, zinTp reached its maximum transcription rate so the fluorescence level stop increasing around 55000 au.


Fluorescence level at 15mins after Cd2+  added

This fig shows the fluorescence level of different cadmium concentration. We can see that BBa_I13522 remained stable from different cadmium concentration, while the fluorescence level of zinTp+GFP increased as the cadmium concentration increased. We could also tell that the transcription rate of zinTp is fairly low when there is no cadmium ion.


Cloning mnt into pSB1C3 plasmid


Mnt gene was successfully cloned into pSB1C3 plasmid


Cloning mnt into pSB1C3 plasmid


smtA gene was successfully cloned into pSB1C3 plasmid

Characterizing of smtA


Cadmium 0/200/500μM Medium

This fig shows that smtA enhances the tolerance level to cadmium ion. In wild type E.coli, OD595 differ from between 0/200/500μM cadmium concentration. E.coli hardly grow in 500μM cadmium medium. However, the E.coli which can express smtA seem to show no significant different between 0 & 200μM cadmium medium.


8 hours after cadmium added

OD of WT E.coli decrease when cadmium concentration increase. The E.coli which can express smtA seem to have stronger resistance to cadmium toxicity and the E.coli concentration show no significant different from 0 to 200μM cadmium medium.



E.coli which could express smtA growth better than wild type in 200μM Cd+2 medium