Team:NYMU-Taipei/ymic1.html

From 2012.igem.org

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<div class="title">Cd+2 Collector</div>
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   <p>Sulfur Oxides (SOX, SO2) is the main precursors of air pollution which is a deteriorating problem nowadays. Producing acid rain and acidified soils, Sulfur Oxides not only result in breathing problems such as asthma, pneumonia, but destroy farm crops, buildings and environment, causing millions in lost each year.<br />
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  <p>We&rsquo;ve  built the endosymbiotic system to clean up cadmium contamination. The engineered organism can collect cadmium ion in the soil and can be easily gather together; moreover, the device won&rsquo;t become another pollution source to our environment.<br />
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    Cadmium contamination is a serious problem in many countries; cadmium can cause respiratory  system damage, renal failure, bones softening, and, maybe the most infamous  case in Asia, Itai-itai disease. The rice absorbed the cadmium in the soil and  the cadmium accumulated in the people eating contaminated rice. The aim of our  project is to build up a system that can collect Cadmium ion in the soil and  can be easily gather together; besides, we don&rsquo;t want our device become another  pollution source to our environment. We found that the endosymbiotic system  between the soil-living amoeba <em>Dictyostelium  discoideum </em>and <em>E.coli </em>would be  our favorite solution.<br />
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      Cadmium ions were absorbed by amoeba then  accumulated inside of E.coli<br />
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    In order to achieve bioremediationwe choose cyanobacteria as our target organ. However, there is no rose without thorn. Due to lost sulfur metabolism functions, We use synthetic biology and gene cloning technique to complete sulfur metabolism pathway inside cyanobacteria.       <br />
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     <img src="http://igem.org/wiki/images/4/4e/Ymis2.gif" alt="" width="428" height="287" /><a href="http://www.genome.jp/kegg-bin/show_pathway?syf00920"><br />
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    http://www.genome.jp/kegg-bin/show_pathway?syf00920</a> (KEGG)</div>
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  <p><span class="subtitle">Why <em>Dictyostelium discoideum</em></span></p>
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<em>Dictyostelium discoideum </em>is a soil-living amoeba, so it is a good choice to collect cadmium ion in soil. In addition, the amoeba naturally has endosymbiont inside the cell. The E.coli living in amoeba accumulates cadmium ions, just  like an organelle, the E.coli acts as a compartment preventing its host from  cadmium poisoning. Furthermore, keeping the E.coli inside the amoeba avoid unexpectedly  gene exchange between engineered E.coli and other wild types bacteriaMoreover, Dictyostelium discoideum chemotaxis is well-studied and can be easily gather by adding cAMP.
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    <p>Single cell amoeba <em>Dictyostelium discoideum</em> gathered  when cAMP was added.<br />
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      http://ppt.cc/CHcw </p>
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Revision as of 01:28, 18 October 2012

NYMU iGEM

Cd+2 Collector

Overview

We’ve built the endosymbiotic system to clean up cadmium contamination. The engineered organism can collect cadmium ion in the soil and can be easily gather together; moreover, the device won’t become another pollution source to our environment.
Cadmium contamination is a serious problem in many countries; cadmium can cause respiratory system damage, renal failure, bones softening, and, maybe the most infamous case in Asia, Itai-itai disease. The rice absorbed the cadmium in the soil and the cadmium accumulated in the people eating contaminated rice. The aim of our project is to build up a system that can collect Cadmium ion in the soil and can be easily gather together; besides, we don’t want our device become another pollution source to our environment. We found that the endosymbiotic system between the soil-living amoeba Dictyostelium discoideum and E.coli would be our favorite solution.


Cadmium ions were absorbed by amoeba then accumulated inside of E.coli

Why Dictyostelium discoideum

Dictyostelium discoideum is a soil-living amoeba, so it is a good choice to collect cadmium ion in soil. In addition, the amoeba naturally has endosymbiont inside the cell. The E.coli living in amoeba accumulates cadmium ions, just like an organelle, the E.coli acts as a compartment preventing its host from cadmium poisoning. Furthermore, keeping the E.coli inside the amoeba avoid unexpectedly gene exchange between engineered E.coli and other wild types bacteria. Moreover, Dictyostelium discoideum chemotaxis is well-studied and can be easily gather by adding cAMP.


Single cell amoeba Dictyostelium discoideum gathered when cAMP was added.
http://ppt.cc/CHcw