Team:NTU-Taida/Project/Sensor

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{{:Team:NTU-Taida/Templates/Header}}{{:Team:NTU-Taida/Templates/Navbar}}{{:Team:NTU-Taida/Templates/Sidebar|Title=Sensor}}{{:Team:NTU-Taida/Templates/ContentStart}}{{:Team:NTU-Taida/Templates/BSHero|Title=Sensor|Content=<p></p>}}
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{{:Team:NTU-Taida/Templates/Header}}{{:Team:NTU-Taida/Templates/Navbar}}{{:Team:NTU-Taida/Templates/Sidebar|Title=Sensor}}{{:Team:NTU-Taida/Templates/ContentStart}}{{:Team:NTU-Taida/Templates/BSHero|Title=Sensor|Content=<p>For the Fat Extinguisher, a fatty acid biosensor FadR is chosen to be coupled with an inducible circuit, which gives response after meal intake. For Brain Guardian, modified cI promoter with temperature-sensitive cI repressor is designed to produce constitutive delivery of peptides. Another thermal promoter Phs is also characterized as a sensor part of the suicide device.</p>}}
==Fatty Acid Biosensor==
==Fatty Acid Biosensor==
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In order to regulate the release of GLP-1, we decide to use a fatty acid biosensor which will switch on when the intestinal environment is filled with fatty nutrient. Our team chose fatty acid as a sensor of metabolic states for two main reasons. First, fatty acid is considered as the major cause to obesity because of their highly calorigenic effect. Second, GLP-1 level increases once the blood glucose concentration rises, but there is no significant GLP-1 secretion when the free fatty acid concentration increases in blood stream. Therefore, we hope to compensate this weakness in the appetite regulating mechanisms. By designing the device that secrets GLP-1 on sensing fatty acid around, we aim to make a dietary bacteria.
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<p style="text-indent: 2em;">In order to regulate the release of GLP-1, we decide to use a fatty acid biosensor which will switch on when the intestinal environment is filled with fatty nutrient. Our team chose fatty acid as a sensor of metabolic states for two main reasons. First, fatty acid is considered as the major cause to obesity because of their highly calorigenic effect. Second, GLP-1 level increases once the blood glucose concentration rises, but there is no significant GLP-1 secretion when the free fatty acid concentration increases in blood stream. Therefore, we hope to compensate this weakness in the appetite regulating mechanisms. By designing the device that secrets GLP-1 on sensing fatty acid around, we aim to make a dietary bacteria.</p>
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[[FIle:NTU-Taida-Project-GLP-1 reaction.png|600px|thumb|center|The difference of physiological GLP-1 response between carbohydrate meal and fat meal.(Ranganath LR,1996)]]
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We use the natural fatty acyl-CoA biosensor, the ''fadBA'' promoter (P<sub>fadBA</sub>) from E. coli as the sensor in our design. ''fadBA'' is one of the β-oxidation gene which E. coli turns on when the fatty acyl-CoA concentration in the surrounding rises. Therefore, we plan to clone the P<sub>fadBA</sub> as the promoter in our device, which enables the bacteria to detect fatty acid in the environment.  
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<p style="text-indent: 2em;">We use the natural fatty acyl-CoA biosensor, the ''fadBA'' promoter (P<sub>fadBA</sub>) from E. coli as the sensor in our design. ''fadBA'' is one of the β-oxidation gene which E. coli turns on when the fatty acyl-CoA concentration in the surrounding rises. Therefore, we plan to clone the P<sub>fadBA</sub> as the promoter in our device, which enables the bacteria to detect fatty acid in the environment. </p>
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However, the baseline expression of the reportor gene (mRFP) downstream of P<sub>fadBA</sub> is so high that we cannot see significant signal rise after induction of fatty acid (oleic acid). Therefore, we decide to lower the baseline expression by overexpression of FadR, an endogenous repressor of P<sub>fadBA</sub>, whose repressive function is antagonized by fatty acyl-CoA. By co-transforming constructs with P<sub>fadBA</sub> and FadR into the bacterial platform, it is made capable of changing gene expression in response to environmental fatty acid concentration and produce GLP-1 when the host is in taking a meal.
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<p style="text-indent: 2em;">However, the baseline expression of the reportor gene (mRFP) downstream of P<sub>fadBA</sub> is so high that we cannot see significant signal rise after induction of fatty acid (oleic acid). Therefore, we decide to lower the baseline expression by overexpression of FadR, an endogenous repressor of P<sub>fadBA</sub>, whose repressive function is antagonized by fatty acyl-CoA. By co-transforming constructs with P<sub>fadBA</sub> and FadR into the bacterial platform, it is made capable of changing gene expression in response to environmental fatty acid concentration and produce GLP-1 when the host is in taking a meal.</p>
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[[FIle:NTU-Taida-Project-Fatty acid sensor.png|600px|thumb|center|The natural fatty acid biosensors working through fadR.]]
==Phs Thermal Promoter ==
==Phs Thermal Promoter ==
''E. coli'' is pretty sensitive and responsive to temperature changes. We brought a thermal sensitive promoter P<sub>hs</sub>, located within dnaG, and transient induced to temperature upshift, to our circuit. As described by Wayne E. Talyor et al., P<sub>hs</sub> can forward upregulation of a series of proteins. (Taylor, 1984) The increase activity of P<sub>hs</sub> promoter is partially compatible with the increase synthesis of sigma factor. The quick respsonse of P<sub>hs</sub> is suitable since its the sigma factor synthesis peaks 10 minutes after the temperature upshift, and so does P<sub>hs</sub> RNA levels. The ratio of increase before and after the temperature rise can be more than 20 folds. On particular note, P<sub>hs</sub> is lack of consensus region over -10 region, which explains its poor activity in 30℃. Thus, P<sub>hs</sub> is marked as a candidate in our circuit, which can only be functional after ingestion into human body. We use this thermal promoter in the suicide device. See the Safety section for more information.
''E. coli'' is pretty sensitive and responsive to temperature changes. We brought a thermal sensitive promoter P<sub>hs</sub>, located within dnaG, and transient induced to temperature upshift, to our circuit. As described by Wayne E. Talyor et al., P<sub>hs</sub> can forward upregulation of a series of proteins. (Taylor, 1984) The increase activity of P<sub>hs</sub> promoter is partially compatible with the increase synthesis of sigma factor. The quick respsonse of P<sub>hs</sub> is suitable since its the sigma factor synthesis peaks 10 minutes after the temperature upshift, and so does P<sub>hs</sub> RNA levels. The ratio of increase before and after the temperature rise can be more than 20 folds. On particular note, P<sub>hs</sub> is lack of consensus region over -10 region, which explains its poor activity in 30℃. Thus, P<sub>hs</sub> is marked as a candidate in our circuit, which can only be functional after ingestion into human body. We use this thermal promoter in the suicide device. See the Safety section for more information.
==Modified cI Promoter with Temperature-Sensitive cI Repressor (CIts)==
==Modified cI Promoter with Temperature-Sensitive cI Repressor (CIts)==
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For the constitutive delivery of GLP-1 peptide in the case of prevention of neurodegenerative diseases, we utilize the composite BioBrick BBa_K098988 designed by Harvard iGEM team 2008 as the backbone of our constitutive delivery module, which gives raised expression of peptide under normal body temperature, 37℃. The original circuit involves a thermosensitive cI repressor (CIts) under the expression of a strong promoter, J23119, and is followed by a CI promoter (P<sub>cI</sub>) region and reporter of desire. Under conditions demonstrated before, its increase of reporter expression after temperature upshift is minimal (1.8 fold). And thus, we design novel cI promoter regions with altered binding affinity to CIts repressor in search of better efficiency. The original cI promoter has two binding regions: OR2 followed by OR1. Here we change these regions into new ones: OR1 and OL1 in order. The binding affinity between OL1 and cI repressor are larger than OR1 and OR2. Therefore, by enhancing the strength of baseline repression level of cI, we can improve the sensitivity to temperature difference of this device. See the Circuit section for further application of this device.
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For the constitutive delivery of GLP-1 peptide in the case of prevention of neurodegenerative diseases, we utilize the composite BioBrick BBa_K098988 designed by Harvard iGEM team 2008 as the backbone of our constitutive delivery module, which gives raised expression of peptide under normal body temperature, 37℃. The original circuit involves a thermosensitive cI repressor (CIts) under the expression of a strong promoter, J23119, and is followed by a CI promoter (P<sub>cI</sub>) region and reporter of desire. Under conditions demonstrated before, its increase of reporter expression after temperature upshift is minimal (1.8 fold). And thus, we design novel cI promoter regions with altered binding affinity to CIts repressor in search of better efficiency. The original cI promoter has two binding regions: OR2 followed by OR1. Here we change these regions into new ones: OR1 and OL1 in order. The binding affinity between OL1 and cI repressor are larger than OR1 and OR2. Therefore, by enhancing the strength of baseline repression level of cI, we can improve the sensitivity to temperature difference of this device. See the [[Team:NTU-Taida/Project/Circuit|Circuit]] section for further application of this device.
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[[FIle:NTU-Taida-Project-CI promoter.png|600px|thumb|center|Modification of cI promoter with new binding sites.]]
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<html>
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<table class='table table-bordered table-hover'>
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  <tr>
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    <td><b>Sequence of different cI binding regions</b></td>
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  </tr>
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  <tr>
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    <td>OL1</td>
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    <td>TACCACTGGCGGTGATA</td>
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  </tr>
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  <tr>
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    <td>OR1</td>
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    <td>TACCTCTGGCGGTGATA</td>
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  </tr>
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  <tr>
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    <td>OR2</td>
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    <td>TAACACCGTGCGTGTTG</td>
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  </tr>
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  <tr>
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    <td>OR3</td>
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    <td>TATCCCTTGCGGTGATA</td>
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  </tr>
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</table>
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</html>
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==Reference==
==Reference==
<ol>
<ol>

Latest revision as of 02:12, 27 October 2012

Sensor

Sensor

For the Fat Extinguisher, a fatty acid biosensor FadR is chosen to be coupled with an inducible circuit, which gives response after meal intake. For Brain Guardian, modified cI promoter with temperature-sensitive cI repressor is designed to produce constitutive delivery of peptides. Another thermal promoter Phs is also characterized as a sensor part of the suicide device.

Contents

Fatty Acid Biosensor

In order to regulate the release of GLP-1, we decide to use a fatty acid biosensor which will switch on when the intestinal environment is filled with fatty nutrient. Our team chose fatty acid as a sensor of metabolic states for two main reasons. First, fatty acid is considered as the major cause to obesity because of their highly calorigenic effect. Second, GLP-1 level increases once the blood glucose concentration rises, but there is no significant GLP-1 secretion when the free fatty acid concentration increases in blood stream. Therefore, we hope to compensate this weakness in the appetite regulating mechanisms. By designing the device that secrets GLP-1 on sensing fatty acid around, we aim to make a dietary bacteria.

The difference of physiological GLP-1 response between carbohydrate meal and fat meal.(Ranganath LR,1996)

We use the natural fatty acyl-CoA biosensor, the fadBA promoter (PfadBA) from E. coli as the sensor in our design. fadBA is one of the β-oxidation gene which E. coli turns on when the fatty acyl-CoA concentration in the surrounding rises. Therefore, we plan to clone the PfadBA as the promoter in our device, which enables the bacteria to detect fatty acid in the environment.

However, the baseline expression of the reportor gene (mRFP) downstream of PfadBA is so high that we cannot see significant signal rise after induction of fatty acid (oleic acid). Therefore, we decide to lower the baseline expression by overexpression of FadR, an endogenous repressor of PfadBA, whose repressive function is antagonized by fatty acyl-CoA. By co-transforming constructs with PfadBA and FadR into the bacterial platform, it is made capable of changing gene expression in response to environmental fatty acid concentration and produce GLP-1 when the host is in taking a meal.


The natural fatty acid biosensors working through fadR.

Phs Thermal Promoter

E. coli is pretty sensitive and responsive to temperature changes. We brought a thermal sensitive promoter Phs, located within dnaG, and transient induced to temperature upshift, to our circuit. As described by Wayne E. Talyor et al., Phs can forward upregulation of a series of proteins. (Taylor, 1984) The increase activity of Phs promoter is partially compatible with the increase synthesis of sigma factor. The quick respsonse of Phs is suitable since its the sigma factor synthesis peaks 10 minutes after the temperature upshift, and so does Phs RNA levels. The ratio of increase before and after the temperature rise can be more than 20 folds. On particular note, Phs is lack of consensus region over -10 region, which explains its poor activity in 30℃. Thus, Phs is marked as a candidate in our circuit, which can only be functional after ingestion into human body. We use this thermal promoter in the suicide device. See the Safety section for more information.

Modified cI Promoter with Temperature-Sensitive cI Repressor (CIts)

For the constitutive delivery of GLP-1 peptide in the case of prevention of neurodegenerative diseases, we utilize the composite BioBrick BBa_K098988 designed by Harvard iGEM team 2008 as the backbone of our constitutive delivery module, which gives raised expression of peptide under normal body temperature, 37℃. The original circuit involves a thermosensitive cI repressor (CIts) under the expression of a strong promoter, J23119, and is followed by a CI promoter (PcI) region and reporter of desire. Under conditions demonstrated before, its increase of reporter expression after temperature upshift is minimal (1.8 fold). And thus, we design novel cI promoter regions with altered binding affinity to CIts repressor in search of better efficiency. The original cI promoter has two binding regions: OR2 followed by OR1. Here we change these regions into new ones: OR1 and OL1 in order. The binding affinity between OL1 and cI repressor are larger than OR1 and OR2. Therefore, by enhancing the strength of baseline repression level of cI, we can improve the sensitivity to temperature difference of this device. See the Circuit section for further application of this device.

Modification of cI promoter with new binding sites.


Sequence of different cI binding regions
OL1 TACCACTGGCGGTGATA
OR1 TACCTCTGGCGGTGATA
OR2 TAACACCGTGCGTGTTG
OR3 TATCCCTTGCGGTGATA

Reference

  1. Zhang F, et al. (2012) Design of a dynamic sensor-regulator system for production of chemicals and fuels derived from fatty acids. Nat Biotechnol 30(4):354-9.
  2. Iram SH, et al. (2005) Unexpected Functional Diversity among FadR Fatty Acid Transcriptional Regulatory Proteins. J Biol Chem 280(37):32148-56.
  3. van Aalten DM, et al. (2000) Crystal structure of FadR, a fatty acid-responsive transcription factor with a novel acyl coenzyme A-binding fold. EMBO J 19(19):5167-77.
  4. Taylor, W. E., D. B. Straus, et al. (1984). "Transcription from a heat-inducible promoter causes heat shock regulation of the sigma subunit of E. coli RNA polymerase." Cell 38(2): 371-381.
  5. Wedler, H. and R. Wambutt (1995). "A temperature-sensitive lambda cI repressor functions on a modified operator in yeast cells by masking the TATA element." Mol Gen Genet 248(4): 499-505
  6. Maeda YT, et al. (2006) Regulatory dynamics of synthetic gene networks with positive feedback. J Mol Biol 359:1107–1124.