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The main circuit is designed to detect the presence of fatty acid in the intestinal environment and produce the peptide drug (GLP-1 in this case) and cell penetrating peptide (CPP) as response. There are two core systems: double repressors and quorum sensing.

Double Repressors

The double repressor system consists of the genes encoded Tet Repressor Protein (tetR) and lac reprresor (lacI). We put tetR downstream of fad promoter (Pfad) and lacI downstream of tet promoter (Ptet). The DNA sequence encoded GLP-1 and CPP are placed downstream of lac promoter (Plac).

structure of MRS